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1

Genetic diversity of the long terminal repeat of bovine leukaemia virus field isolates

100%
Pluta A., Rola-Luszczak M., Olech M., Kuzmak J.
Bulletin of the Veterinary Institute in Puławy
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2015
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tom 59
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nr 4
In this study the sequences of the long terminal repeat (LTR) of field isolates of the bovine leukaemia virus (BLV) were analysed. These isolates came from emerging cases of BLV infection in cattle from herds having BLV-free status. We found several sequence variations within regulatory motifs in the LTRs like GRE, DAS and interferon binding site. These mutations can possibly affect transcriptional activity of the virus, leading to its silencing.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Leucocyte acid phosphatase and selected haematological indices in BLV infected cows

72%
Kaczmarczyk E., Czarnik U., Bojarojc B., Walawski K.
Journal of Applied Genetics
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1999
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tom 40
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nr 2
3

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay

58%
Bicka L., Kuzmak J., Kozaczynska B., Plucienniczak A., Skorupska A.
Acta Biochimica Polonica
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2001
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tom 48
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nr 1
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and ex­pressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immuno­blotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for de­tection of BLV antibodies in the infected cattle.
4
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Application of revertase assay for detection of EBL virus in cattle lymphocytes

58%
Reichert M., Grundboeck-Jusko J., Stec J., Rulka J., Buzala E.
Bulletin of the Veterinary Institute in Puławy
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1992
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tom 36
5

Leucocyte acid phosphatase and metabolic efficiency of phagocytes in the first lactation trimester of cows from a leukaemic herd

58%
Kaczmarczyk E., Bojarojc-Nosowicz B., Fiedorowicz A.
Journal of Applied Genetics
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2005
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tom 46
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nr 1
In Black-and-White cattle, polymorphism of acid phosphatase (AcP) of blood leukocytes is determined by a pair of autosomal alleles. The aim of the study was to determine the relationship between AcP polymorphism and the metabolic efficiency of phagocytes in the first months after calving of cows naturally infected with the bovine leukaemia virus. The studied population consisted of 91 Black-and-White cows aged 3-6 years, from one herd. Enzootic bovine leukaemia (EBL) was diagnosed with the immuno-enzymatic ELISA method and a PCR molecular test. Additionally, agarose gel electrophoresis and the cytochemical method were used to determine the AcP polymorphism and activity in leukocytes. The metabolic activity of phagocytes was determined by the nitroblue tetrazolium (NBT) reduction test. Significant differences in metabolic efficiency of granulocytes were observed between cows representing different AcP phenotypes. No significant differences in levels of the analysed indices were observed between the EBL-positive and EBL-negative cows and between the three subsequent months after calving.
6
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Determination of IgG1, IgG2, and IgM antibody titres in the sera of bovine leukaemia virus - infected cows using the modified indirect ELISA

58%
Kozaczynska B.
Bulletin of the Veterinary Institute in Puławy
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1996
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tom 40
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nr 2
The aim of this study was to determine the titre of IgG₁, IgG₂, and lgM antibodies against bovine leukaemia virus (BLV) in the serum of either experimentally or naturally infected animals. Experimental infection performed in calves injected intravenously with blood from leukaemic cattle. The presence of BLV antibodies belonging to lgG₁, IgG₂, and IgM classes were studied for 7 months using the ELISA. Subsequent examinations were carried out on 126 cows from breeding stations extensively affected with BLV. Serological examinations of antibodies against BLV was performed by the ELISA. In the sera of experimentally infected animals IgM antibodies attained a maximum level in stage 2 (42 to 64 d) but lgG₁ antibodies during the last stage of immunogenesis ( 182 to 224 d). No statistically significant differences in IgG₂ antibodies throughout the entire experimental period were found. In the case of natural infection the IgG₁ and IgG₂ antibody levels increased concomitantly with the total antibody titres in the serum; however, IgG₂ values were significantly lower throughout the entire period of the study. In contrast, the highest lgM titre value was found in animals with the lowest level of total antibodies. Enzootic bovine leukaemia (EBL) is a neoplastic disease, associated with unlimited and irreparable proliferous alterations induced by bovine leukaemia virus (BL V) (25). Lymphocytes B are regarded as the target cells although T lymphocytes may also be infected (27, 30). Bovine leukosis reveals a long-lasting latency period involving integration of the viral genetic material with the host cell genome in the proviral form. A chronic lymphocytosis develops in about 30% of infected animals (1, 6, 27) whereas a small percentage of infected cattle develops tumours (6, 7). The production of antibodies against BLV is a permanent feature in the affected animals. The influence of BLV infection on immunoglobulin levels in sera has been extensively investigated but the results are contradictory (8, 9, 12, 13, 22, 29, 31). These investigations have mainly focused on total lg level measurements; consequently conflicting results concerning the behaviour of immunoglobulins regarded as the specific antibodies to the virus have been obtained.
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