Wyniki wyszukiwania

1

Use of the rep-PCR technique for differentiating isolates of rhizobacteria

100%

Lisek A., Sas Paszt L., Oskiera M., Trzcinski P., Bogumil A., Kulisiewicz A., Malusa E.

Journal of Fruit and Ornamental Plant Research

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2011

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tom 19

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nr 1

In this study, the rep-PCR technique was used to differentiate isolates of bacteria belonging to genus Pseudomonas and phosphate-dissolving bacteria collected from the root vicinity of apple and sour cherry trees. DNA amplification was carried out with complementary primers for repetitive sequences: REP (repetitive extragenic palindromic sequence), ERIC (enterobacterial repetitive intergenic consensus) and the BOX element. The most differentiated DNA profiles were observed when using REP1R-I and REP2-I primers, in reactions with which 25 different DNA patterns were obtained for 28 isolates. In reactions with the primers ERIC1R and ERIC2 or BOXA1R, 24 and 22 patterns were obtained, respectively. Following the use of all the primers, no differences were found in the DNA profiles of two isolates of Pseudomonas bacteria and three isolates of phosphate-dissolving bacteria. This result suggests that the isolates in which no DNA polymorphism was observed belong to the same bacterial strain.

2

Genetic features of clinical Pseudomonas aeruginosa strains

100%

Wolska K., Szweda P.

Polish Journal of Microbiology

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2009

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tom 58

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nr 3

255-260

The genetic features of each isolate were determined by enterobacterial repetitive intergenic consensus (ERIC) primer sequences used in PCR and by searching for six virulence genes (alg D, las B, tox A, plc H,plc N, exo S). 49 (79%) of the isolates were distributed in three ERIC PCR subgroups and showed 62% of similarity. The remaining 13 strains generated unique patterns. The first subgroup was primarily composed of isolates from faeces, these strains indicated over 70% relationship with the next subgroup, and primarily contained strains isolated from wounds and bronchial washings and the last subgroup contained strains isolated from wounds and urine. The unique strains were isolated mainly from urine. Statistical analysis indicated that variations in distribution of virulence genes in P. aeruginosa isolates with respect to strain origin and genomic subgroups were not significant. In the group of 49 strains, 100% gave a positive reaction to alg D, las B and plc H genes, 91.8% to tox A and plc N genes and 83.7% to exo S gene. Among the strains that generated unique (ERIC-PCR) patterns, 69.2% gave a positive reaction to alg D gene, 84.6% to las B gene, 76.9% to tox A, plc N and plc H genes, and 46.15% to exo S gene.

3

A comparative evaluation of PCR ribotyping and ERIC PCR for determining the diversity of clinical Pseudomonas aeruginosa isolates

100%

Wolska K., Szweda P.

Polish Journal of Microbiology

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2008

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tom 57

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nr 2

Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively. The result showed that ERIC PCR analysis is a more discriminatory method than PCR ribotyping analysis and traditional serotyping scheme. We suggest that maximum discrimination can be achievied by a combination of these methods.

Ograniczanie wyników

2 Polish Journal of Microbiology

1 Journal of Fruit and Ornamental Plant Research

2 Szweda P.

2 Wolska K.

1 Bogumil A.

1 Kulisiewicz A.

1 Lisek A.

1 Malusa E.

1 Oskiera M.

1 Sas Paszt L.

1 Trzcinski P.

1 2011

1 2009

1 2008

Use of the rep-PCR technique for differentiating isolates of rhizobacteria

Genetic features of clinical Pseudomonas aeruginosa strains

A comparative evaluation of PCR ribotyping and ERIC PCR for determining the diversity of clinical Pseudomonas aeruginosa isolates