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Most flowering plants, including important crops, require double fertilization to form an embryo and endosperm, which nourishes it. Independence from fertilization is a feature of apomictic plants that produce seeds, from which the plants that are clones of the mother plant arise. The phenomenon of apomixis occurs in some sexual plants under specific circumstances. Since the launch of a fertilization-independent mechanism is considered a useful tool for plant breeding, there have been efforts to artificially induce apomixis. We have been able to produce fertilization-independent endosperm in vitro in Arabidopsis over the last few years. This paper demonstrates the methods of improving the quality of the endosperm obtained using plant and mammalian steroid hormones. Additionally, it shows the study on the autonomous endosperm (AE) formation mechanism in vitro. This paper examines the effect of exogenous steroid hormones on unfertilized egg and central cell divisions in culture of unpollinated pistils of Arabidopsis Col-0 wild-type andfie-1 mutant. All media with hormones used (estrone, androsterone, progesterone, and epibrassinolide) stimulated central cell divisions and fertilization-independent endosperm development. The stages of AE development followed the pattern of Arabidopsis thaliana wild type after fertilization. Subsequent stages of AE were observed from 2-nuclear up to cellular with the most advanced occurring on medium with 24-epibrassinolide and progesterone. The significant influence of mammalian sex hormones on speed of AE development and differentiation was noticed. Using restriction analysis, the changes in methylation of FIE gene was established under in vitro condition. The authors of this paper showed that Arabidopsis thaliana has a high potency to fertilization-independent development.
Presence of five carboxypeptidases was found in endosperm of germinating triticale grains, while two of them in scutellum. Changes of their activities during four days of germination suggest that carboxypeptidase II plays an important role at initial stage of germination, while carboxypeptidases I and III - at subsequent stages of the process. High activity of carboxyt peptidase II both in scutellum and endosperm of dry grains accompanied by its decrease during germination, and on the other hand, the appearance of carboxypeptidases I and III activities at the 2nd and 3rd day of the process seems to confirm such functions of these enzymes. Experiments with GA3 indicated that carboxypeptidase I was synthesized in scutellum, and carboxypeptidase III - in aleurone layer. Carboxypeptidases I and II cleave N-CBZ-Phe-Ala, and carboxypeptidase III - N-CBZ-Ala-Met and N-CBZ-Ala-Phe as substrates with the highest rate.
In Solanum muricatum Aiton the development of cellular endosperm preceded the divisions of the zygote, and 5 days after pollination (dap) it consisted of several cells. The mature endosperm accumulated lipid and protein bodies. The separation and secretion zone on the embryo/endosperm interface was noted at 20 dap. The first division of the zygote occurred at 7 dap. The apical cell of the 2-celled proembryo produced the embryo proper, and the basal one gave rise to the suspensor, the central part of the root cap (columella) and the connecting layer of the embryonic root meristem. Four-celled proembryos were observed with the cells in linear arrangement at 8 dap. The first globular embryos were found at 16 dap, heart-stage embryos at 26 dap, torpedo-shaped embryos at 30 dap, and circinate embryos at 33 dap. After 56 dap no further changes were observed in the anatomical structure of the embryos. The testa of mature seeds was composed of the outer epidermis of the integument and a layer of obliterated parenchyma and endothelium.
TSK gel permeation chromatography of non-granular starches, amylopectin chain-length distributions measured by HPAEC-PAD, and DSC characteristics of starches of maize endosperms possessing different alleles at the amylose-extender (ae) loci were studied. GPC of non-granular starches through Toyopearl columns showed that elution profiles for 5 ae mutants, Oh43 inbred line ae (standard ae), ae- RWB-2 and ae-RWB-3, and W23xL317 hybrid line ae-PP and ae-Bol 561, were similar to a commercial ae starch, Hylon VII and different from Hylon ae starch and normal maize starches. The elution profile for W23xL317 ae-emll was similar to Hylon and different from Hylon and normal maize starches. HPAEC-PAD of isoamylase-debranched starches showed that the 5 ae mutants were uniquely ae type similar to Hylon VII and different from Hylon V. W23xL137 ae-emll had the amylopectin chain-length distribution similar to Hylon . Gelatinization temperatures (Tp) of the ae starches measured by a Setaram Micro DSC III were high compared with the normal counterpart starches except for Oh43 ae-RWB-1 starch. Oh43 ae-RWB-1 starch had structure and thermal characteristics similar to the normal maize starch.
In previous research, changes in concentrations of the physiologically essential elements K, Na, Ca, Mg, Fe, Mn, Zn, Cu and B in developing ovules of Clivia miniata Regel (central vacuole sap, endosperm tissue and embryo) were examined. Now the authors present results of measurements of change in molybdenum concentration in the central vacuole and micropylar and chalazal parts of endosperm during development of C. miniata ovules. The analysis was performed with the use of flameless atomic absorption spectrometry, with Zeeman background correction and using pyrolytically coated graphite tubes. The dimensions of the embryos and their age (counted from the day the perianth wilted to the day of sampling) were the adopted developmental criteria. It was established that (1) during the inhibitional phase of embryo growth, the concentration of Mo in the central vacuole sap varied from 0.021 to 0.052 µg/ml; (2) the Mo concentration in the micropylar part of the endosperm was in the range of 0.032-0.085 µg/g fr.wt. and increased during the exponential and stationary phases of embryo growth; (3) the same increasing tendency was found in the chalazal part of the endosperm tissue (concentration range: 0.074-0.11 µg/g fr.wt.); (4) the concentration of Mo in the chalazal part of the endosperm was higher than in the micropylar part during the examined period of embryo growth.
In Consolida regalis L. the development of the embryo sac conforms to the Polygonum type. In the mature embryo sac, the egg apparatus and secondary nucleus are of normal appearance. The synergids are ephemeral and disappear soon after fertilization. The antipodal cells are large and persistent but remain uninucleate and show rhythmical growth. The volumes of antipodal nuclei correspond with ploidy levels from n to 256n. Cytochemical tests indicate the presence of high amounts of proteins, lipids, polysaccharides, RNA and DNA in the antipodals. They degenerate in the cellular endosperm stage. The endosperm is nuclear; centripetal wall formation starts at the micropylar end and progresses towards the chalaza. Embryogeny is of the Solanad type. The mature embryo lacks organs at shedding of seeds.
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