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Ten cows with and 5 cows without puerperal endometritis were treated with 20 μg GnRH analogue, buserelin, intramuscularly. Blood samples were taken every 20 min for 8h before and for 8h after injection of GnRH. All cows responded to GnRH with significant increase of LH 20 min after treatment. The LH peak was significantly lower in cows with puerperal endometritis than that in clinically normal cows (27.91 ± 5.47 ng/ml vs 80.60 ± 17.2 ng/ml; p ≤ 0.01). The total AUC of LH was also significantly smaller in cows with puerperal endometritis compared to cows without it (p ≤ 0,01). The results suggest that the delayed resumption of ovarian function in cows with uterine infection may be caused by reduced responsiveness of the pituitary to endogenous GnRH. Further studies are needed in order to investigate the mechanism of this phenomenon.
The aim of this study was to determine the in vitro resistance of aerobic bacteria isolated from the uterine pathological secretion of 312 dairy cows with clinical metritis and clinical endometritis to antibiotics. Animals with pathological discharges from the vagina observable between the 7th and 50th day after parturition were diagnosed clinically per vaginam and per rectum and then swabs from uteri lumen were aseptically collected. Bacteriological examinations of swabs were performed according to commonly accepted rules. Sensitivity to antibiotics was tested by the disk diffusion method and performed according to CLSI (formerly NCCLS) guidelines in Mueller-Hinton agar. The bacteria isolated were mostly Arcanobacterium pyogenes, Escherichia coli, non E. coli Gram-negative rods, Streptococcus species and Staphylococcus species. Strains of Arc. pyogenes were the most susceptible to amoxicillin/clavulanic acid (97.3%), ceftiofur (98%) and bacitracine (96.7%). E. coli isolates were the most susceptible to norfloxacin (100%), marbofloxacin (100%), rifaximin (97%), gentamycin (96%) and amoxicillin/clavulanic acid (95.5%). Other Gram-negative bacteria were the most sensitive to norfloxacin (100%), neomycin (100%) and cefoperazon (95%). Streptococcus species were the most susceptible to amoxicillin/clavulanic acid (94.6%), ampicillin (92.3%), norfloxacin (92%), cephapirine (88%), cefoperazone (86.5%), rifaximine (85.7%) and penicillin (84.9%). The highest in vitro activity against Staphylococcus spp. was demonstrated by amoxicillin/clavulanic acid (100%), norfloxacin (100%), neomycin (93.6%) and cefoperazone (85.7%). Arc. pyogenes were the most resistant to oxytetracycline and cloxacillin, E. coli to ampicillin and cephapirin, non-E. coli Gram-negative rods to ampicillin and cephapirin, Streptococcus spp. to neomycin and oxytetracycline, and Staphylococcus spp. to ampicillin.
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Predisposing factors for puerperal metritis in cows

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The investigation was performed on 90 multiparous cows in four farms. Animals were almost identically fed and had similar milk yield during last year (6000 kg milk). Blood samples were taken 3 times (-3, -2, -1 week) before and 2 times (+1, +3 week) after parturition. Some biochemical parameters were determined. In all farms the increase of free fatty acids level was observed after parturition. The decrease in cholesterol concentration and triglyceride level after calving were accompanied. Differences in valucs of analysed parameters (AST, FFA, glucose) in cows with correct and disturbed post partum period were observed before parturition.
The aim of the study was to describe a new method used for diagnosing uterine disorders and to present preliminary results regarding the incidence of this kind of endometritis in dairy cows. For its diagnosis, samples must be taken from the lumen of the uterine horns. This requires a special tool which consists of a catheter open at both ends/on both sides, a mandrel and a brush for the collection of samples. After sampling the brush is rolled on cytological glass, and the smear is stained using one of the classic methods. Following this procedure, a number of polimorphonuclear leucocytes (PMNL), other white cells and epithelial cells are counted. In order to diagnose the disorder, the percentage of PMNL is considered. In our trials, conducted in two experimental herds, subclinical endometritis was diagnosed in 69.7% of cows in one herd and in 38.3% of cows in the other herd during the fourth week post partum. Two weeks later, the incidence of this disease dropped to 43.3% and 17%, respectively. Only in 12.1% and 8.7% of cows after parturition the number of PMNL grew with the passage of time. More research is needed to evaluate the influence of cytological endometritis on reproductive performance and to examine the pathological and physiological nature of this disorder.
The study was conducted in the years 2006–2008. The experimental materials comprised 368 Holstein-Friesian cows purchased from Germany as in-calf heifers. The objective of this study was to determine the health status of cows in a commercial herd kept in the Olsztyn region, based on the type and incidence of diseases that occurred during three consecutive lactations, and to analyze the effect of these diseases on milk yield and composition in the first, second and third lactation cycle. The cows were divided into five groups: HEA – clinically healthy cows (showing no disease symptoms), MAS – cows with mastitis, LAM – cows with foot/leg defects and lameness, REP – cows with reproductive problems (retention of the placenta, endometritis, ovarian cysts), MET – cows with metabolic diseases (ketosis, abomasal displacement). It was found that the most common diseases during three consecutive lactations in the investigated herd were endometritis (37.63%) which occurred soon after calving (on day 18 post-partum), mastitis (35.48%), formation of ovarian cysts (10.10%), ketosis (8.39%) and leg/foot defects (6.44%). Retention of the placenta and abomasal displacement were diagnosed much less frequently (1.62% and 0.34% respectively). The percentage of healthy cows decreased in successive lactations (19.81% in the first lactation, 12.28% in the second lactation, 6.22% in the third lactation). During each lactation, more than one third of cows suffered from mastitis. The proportion of cows showing the symptoms of ketosis increased with age, from 5.12% in the first lactation to 12.23% in the third lactation. The highest yields of milk and milk components over a 305-day lactation cycle were noted in cows with reproductive diseases (ROZ). Foot/led defects and lameness (LAM), mastitis (MAS) and metabolic diseases (MET) had the most significant effect on a decrease in milk production.
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