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The main objective of the present study was to investigate the effect of spray- and freeze-drying techniques on the microencapsulation of a gallic acid compound using the acid-hydrolyzed low dextrose equivalent potato starch as a wall material. During the experiment, it was possible to achieve encapsulation efficiency of 70–84% for the freeze-dried and 65–79% for spray-dried samples, without statistically signifi cant difference (P>0.05) in the encapsulation efficiency between the mentioned methods. Spray-dried samples formed spherical capsules with a higher number of micropores. Meanwhile, freeze-dried samples were shapeless, exposed larger pore volume (from 2.4×10–3 to 9.5×10–3 cm3 /g against 1.2×10–3 4.9×10–3 cm3 /g; analyzed by Barrett-Joyner-Halenda method) and overall higher surface area (0.632–1.225 m²/g against 0.472–1.296 m²/g; analyzed by Barrett-Joyner- -Halenda method). Due to this fact, more gallic acid molecules were exposed to environmental factors and can be counted as losses. In addition, freeze-dried samples demonstrated lower water activity than spray-dried samples (0.075±0.014 against 0.178±0.008). Results showed that it is not practical to use freeze-drying for modelling encapsulation for food industry without a special necessity for protection of easily degradable chemical compounds. The present work makes a basis for the future studies of the microencapsulated phenolics application in food production.
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Somatic seeds of Plantago asiatica L.

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Somatic seeds of Plantago asiatica L. were produced for the first time. Shoot-tips isolated from in vitro obtained 4-week shoots were encapsulated using sodium alginate and calcium chloride. Capsules with or without sucrose and with and without cytokinin - indole-3-butyric acid (IBA) were used. Sucrose presence in capsules very distinctly influences somatic seeds of Plantago asiatica germination and their conversion into plants. However, addition of IBA to capsules has not clear influence on the ability of plant regrowth. Plantlets transplanted to soil grew to phenotypically normal plants.
The objective of this study was to evaluate effectiveness of Ovalbumin-LHRH-7 (OL) protein when injected in crude, purified, free or encapsulated forms and using a single vaccination protocol along with CpG, inulin and saponin adjuvants. Fifty six C57BL/6 mice in seven groups (n=8) received various treatments and doses: Group 1 was control; Group 2 and 3 were injected twice with purified or crude OL protein, respectively, 4 wks apart. Group 4 and 5 were injected only once with purified or crude OL protein, respectively. Group 6 was injected only once with a mix of purified OL protein and encapsulated purified OL protein. Group 7 was injected only once with a mix of crude OL protein and encapsulated crude OL protein. There was an immunization effect observed on the I125 LHRH % binding (P<0.05). Antibodies (Abs) against LHRH were identified on week 5 of immunization in groups 2, 3 and 4. Boosting at week 5 caused a significant increase in LHRH antibody (Ab) concentrations in groups 2 and 3. Numbers of pregnant animals and prengnancy rates were suppressed in all treatment groups at various degrees (P<0.05). Numbers of pups born were affected by immunization (P<0.05).Concluding, immunization with OL protein generated either biological or both immunological and biological effects in the most of treatment groups. The study confirmed the earlier findings that purified OL protein with CpG adjuvant is effective in inducing immune response and suppressing reproductive functions. However, the original idea that the non-capsulated antigen/adjuvant mix would work as primary injection, while encapsulated counterpart would mimic booster injections in a single vaccination protocol could not be confirmed in this study. Further studies to determine affecting factors for single-injection LHRH immunization are needed.
Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3- acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA3). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.
An efficient protocol for storage of Polypodium vulgare rhizome shoot tips was developed. The best result was observed when the rhizomes were pretreated with 2 mg l-1 abscisic acid/24 h and dehydrated 10 h in hypertonic solution of 20% mannitol before shoot tip isolation. Those explants were encapsulated and stored for 2 months. Plantlets of good quality regenerated after the capsules were transferred to Murashige Fern Multiplication Medium.
This study assessed regrowth of micropropagated Rhodiola Kirilowii buds after encapsulation of axillary buds and differentiating callus in calcium alginate hydrogel and low-temperature preservation. This method of micropropagation was applied to obtain enough plant material for studies on chemical compounds of Rhodiola Kirilowii, a plant difficult to obtain from its natural environment. Axillary buds and differentiating callus were encapsulated in calcium alginate and stored at 4°C for 1 to 15 weeks and then transferred to hormone-free MS medium. The best results were obtained after six weeks of preservation: 100% of the encapsulated explants survived and developed into shoots and plantlets after subculture on basal MS medium. Longer storage of encapsulated axillary shoot buds decreased their regrowth, but the duration of storage of encapsulated differentiating callus had no significant influence on its survival.
The study evaluated two methods of conserving Rubus chamaemorus L. shoot cultures: medium-cold storage and encapsulation of shoot buds. Cold-stored explants were transferred every 12 weeks to proliferation medium and their multiplication rate was observed after the first passage. Shoot cultures stored at 4°C under low light intensities for 12 months without intervening subculture survived with 90% viability. Axillary buds obtained from in vitro shoot culture of cloudberry were encapsulated in calcium alginate hydrogel. Regrowth response of encapsulated buds was estimated. Encapsulated buds stored at low temperature in the dark survived for up to 3 months without loss of viability. The fidelity of subcultured plantlets was also evaluated by phytochemical analysis (fingerprinting) of some phenolic compounds and morphological observations. Preliminary trials in the botanical garden showed that plantlets of Rubus chamaemorus derived in vitro can be used for ex situ germplasm conservation.
W pracy przedstawiono podstawowe informacje na temat unieruchamiania komórek drobnoustrojów. Scharakteryzowano właściwości alginianu jako najpowszechniej stosowanego materiału do unieruchamiania komórek oraz omówiono podstawowe techniki kapsułkowania materiałów komórkowych. Przedstawiono także przykłady praktycznego wykorzystania kapsułkowanych komórek.
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