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1

Androgenic switch in barley microspores

100%
Maraschin,de S. F., Gaussand G., Caspers M., Pulido A., Olmedilla A., Graner A., Wang M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2005
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tom 47
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nr 1
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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High frequency of doubled haploid plant production in spelt wheat

100%
Lantos C., Jenes B., Bona L., Cserhati M., Pauk J.
Acta Biologica Cracoviensia. Series Botanica
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2016
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tom 58
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nr 2
This is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing importance not only in applied research but also in organic farming and changing nutritional standards. In this study, an efficient AC protocol has been described for ‘GK Fehér’ spelt wheat. The number of AC-derived embryolike structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per 100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of ‘GK Fehér’ 306 green plantlets were produced in vitro and 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while a few seeds were harvested from seven partially fertile plants.
3

Regeneration of Pelargonium x hederaefolium 'Bonete' from petiole explants

100%
Wojtania A., Gabryszewska E., Marasek A.
Acta Physiologiae Plantarum
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2004
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tom 26
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nr 3
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures

84%
Ponitka A., Slusarkiewicz-Jarzina A.
Acta Societatis Botanicorum Poloniae
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2009
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tom 78
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nr 3
209-213
The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Improvement of medium for Miscanthus x Giganteus callus induction and plant regeneration

84%
Plazek A., Dubert F.
Acta Biologica Cracoviensia. Series Botanica
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2010
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tom 52
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nr 1
105-110
Miscanthus × giganteus tissue cultures brown quickly and strongly, probably as an effect of oxidation of phenolics produced by plant tissue. The aim of the work was to improve the medium composition for callus induction and plant regeneration of M. giganteus, with two experiments. The first one was aimed at developing a protocol to inhibit biosynthesis and oxidation of phenolics. Callus was induced from immature inflorescences on basal MS medium with 6.5 mg · dm-3 2,4-D, 0.25 mg · dm-3 BAP, 500 mg · dm-3 casein hydrolysate and 30 g · dm-3 sucrose (control medium), and this medium supplemented with one of the following: 200 mg · dm-3 chitosan, 65 g · dm-3 banana pulp (BP), 100 mg · dm-3 cysteine, or 30 g · dm-3 honey instead of sucrose. Plant regeneration used basal MS medium supplemented with 30 g · dm-3 sucrose and 0.2 mg · dm-3 BAP or 0.05 mg · dm-3 KIN. The second experiment was to verify whether BP and honey increase callus production and plant regeneration. For callus induction the explants were put on the control medium and medium supplemented with one of these: 65 g · dm-3 BP, 30 g · dm-3 honey instead of sucrose, or 65 g · dm-3 BP + 30 g · dm-3 honey instead of sucrose. The regeneration medium was basal MS medium supplemented with 0.05 mg · dm-3 KIN and 30 g · dm-3 sucrose or 30 g · dm-3 honey. Tissue browning was independent of medium content, but there was less browning on medium supplemented with honey. In the first experiment, regenerated plants were obtained only on basal MS medium with 30 g · dm-3 sucrose and 0.05 mg · dm-3 KIN. In the second experiment, MS medium containing BP and honey instead of sucrose was the best medium for callus induction, and regeneration from these calli was highest on basal MS with KIN and honey.
6

Flax [Linum usitatisimum L.] - A plant system for study of embryogenesis

84%
Pretova A., Obert B.
Acta Biologica Cracoviensia. Series Botanica
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2003
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tom 45
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nr 1
Embryogenesis is a critical stage of the sporophytic life cycle, during which the basic body plan of the plant is established. Although zygotic embryogenesis is induced by fusion of the sperm and egg nuclei, plant cells can initiate embryo development without fertilization. For example, cultured somatic and male gametic cells can be induced to undergo somatic and microspore embryogenesis, respectively. Embryogenesis in vitro represents a powerful tool to manipulate plant development. After characterizing in situ embryo development in flax, we followed the cytological, morphological and some biochemical features of zygotic embryo development in embryo cultures. We also induced direct and indirect somatic and gametic embryo formation in flax. There is a strong indication that somatic and gametic embryogenesis is a stress response and that it is a way the plant cell realizes its survival strategy under completely changed and unusual conditions.
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