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In Consolida regalis L. the development of the embryo sac conforms to the Polygonum type. In the mature embryo sac, the egg apparatus and secondary nucleus are of normal appearance. The synergids are ephemeral and disappear soon after fertilization. The antipodal cells are large and persistent but remain uninucleate and show rhythmical growth. The volumes of antipodal nuclei correspond with ploidy levels from n to 256n. Cytochemical tests indicate the presence of high amounts of proteins, lipids, polysaccharides, RNA and DNA in the antipodals. They degenerate in the cellular endosperm stage. The endosperm is nuclear; centripetal wall formation starts at the micropylar end and progresses towards the chalaza. Embryogeny is of the Solanad type. The mature embryo lacks organs at shedding of seeds.
The effect of auxin treatment on the formation of apomictic – endospermless grains in Poa pratensis was investigated in the study. Four polish cultivars: Skiz, Eska 46, Alicja, Ani as well as six breeding lines: POB 13, SK-W-33, SKW-15, SKW-35A, SK-W-35A and SK-W-35B were tested by embryological methods to reveal the reproduction pathway in greenhouse and field conditions. The obtained results showed that four genotypes, i.e. Alicja, Ani, Eska 46 and SK-W-35B are facultative apomictic and the other have formed seeds only on the apomictic way. In facultative apomicts it was found that in many cases both endospermless ovules containing embryo sacs with embryos or embryo sacs with well developed endosperm were formed. In the case of apomictic genotypes only endospermless seeds were observed.
A three-step procedure was adopted for the induction of gynogenesis in two cultivars of mulberry (Morus alba L.). This includes in vitro flowering, inflorescence segment culture and isolated ovary culture. In the third step, that is, isolated ovary culture, the cultured ovaries burst and an embryo emerged from the ovary. The present paper investigates the ontogeny of the developing gynogenic embryo. The study confirmed that the gynogenic embryo emerged from the egg cell. Before the onset of division of the egg, all other cells of the embryo sac degenerated in the majority of ovules, but in exceptional cases the polar nuclei will be retained along with the dividing egg cell. The presence of the gynogenic embryo along with free-nuclear autonomous endosperm is the most important feature of the present investigation. Autonomous endosperm is formed from either the polar nuclei or secondary nucleus. In both cultivars used for the experiment, the percentage of ovaries showing proembryo induction during inflorescence segment culture is much higher than that of ovaries producing gynogenic plants during isolated ovary culture. This suggests the degeneration of some gynogenic embryos during the initial stages of induction.
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