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1

Triploids in cucumber: V. The effect of the homo- and heterozygous states on some traits of triploids grown in the field and under cover

100%

Sarreb D. A., Mackiewicz H. O., Malepszy S., Bolesta E.

Folia Horticulturae

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2000

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tom 12

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nr 1

2

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Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

88%

Wojciechowska B., Pudelska H.

Journal of Applied Genetics

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1995

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tom 36

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nr 4

In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations. Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation. The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants). Plants obtained via embryo callus culture showed good vegetative vigour and well-developed root system. Spike morphology of all plants resembled that of rye. Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The heteromorphic bivalent-like configurations occurred in five plants in 0.01-0.02 per cell. The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with a chiasma frequency of 9.36-17.75 per cell. All plants, with 14 and 28 chromosomes, were completely sterile both in backcrosses and when selfed.

3

Methods of embryo scoring in in vitro fertilization

75%

Baczkowski T., Kurzawa R., Glabowski W.

Reproductive Biology

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2004

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tom 04

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nr 1

4

Influence of genotype and medium on culture of immature zygotic embryos of Cucumis sativus L. and Cucumis melo L.

75%

Ondrej V., Navratilova B.

Acta Biologica Cracoviensia. Series Botanica

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2000

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tom 42

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nr 2

Four genotypes of Cucumis sativus and Cucumis melo were used to make embryo culture. Embryos were excised from unripe fruits 1, 2 and 3 weeks after self-pollination. MS (Murashige and Skoog, 1962) basal medium was supplemented with ascorbic acid, growth regulators and 2% or 3% sucrose. Higher regeneration was achieved on medium with lower concentration of sucrose and phytohormones. Plants were regenerated from immature embryos isolated 1 week after self-pollination for C. sativus (Sm-6514) and 2 weeks after self-pollination for C. melo.

5

Evaluation of the developmental toxicity of gentamicin using postimplantation rat whole embryo culture

75%

Biernacki B., Wlodarczyk B., Minta M.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 2

The embryotoxicity of gentamicin was evaluated with rat whole embryo culture (WEC) assay. Rat embryos displaying 1-3 somites were explanted on gestation day (GD) 9.5 and cultured for 48 h in rat serum. Gentamicin sulfate was added to the culture medium at a concentration of 1, 10 and 100 μg/ml. At the end of the cultivation five endpoints were evaluated for each viable embryo: yolk-sac diameter, crown-rump length, head length, somites number and morphological score. Gentamicin at the concentration of 100 μg/ml was embryotoxic and caused growth retardation in rat embryos. At the concentration of 10 μg/ml about 45% of embryos showed haematomas. The lowest dose of antibiotic (1 μg/ml) did not interfere with embryonic development in vitro.

6

A comparison of callus induction and plant regeneration from different embryo explants of triticale [x Triticosecale Wittmack]

75%

Birsin M. A., Ozgen M.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 2

Immature, mature and endosperm-supported mature embryos of six triticale cultivars (BDMT-98-8S, Melez-2001, Mikham-2002, Presto, Tacettin Bey and Tatlicak-97) were cultured in vitro to compare the levels of callus induction and plant regeneration. Immature embryos, 15-18 days after anthesis, were aseptically excised and placed with the scutellum upwards on a callus culture medium consisting of Murashige and Skoog (MS) mineral salts supplemented with 2 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D). Mature embryos were aseptically excised from the imbibed seeds and placed scutellum up on MS medium supplement with 2 mg l-1 2,4-D. Endosperm-supported mature embryos were moved slightly in the imbibed mature seeds. The seeds with moved embryos were placed furrow downwards in dishes containing 8 mg l-1 2,4-D for callus induction. The developed calli and regenerated plants were maintained on hormone-free MS medium. Variability among the genotypes was observed for all the types of embryo culture. Immature embryos from "Presto"and endosperm-supported mature embryos from "Mikham 2002" had excellent regeneration capacities (92.0% and 97.3%, respectively) and the highest number of plants regenerated growing in soil (9 and 13, respectively). A comparison of the responses of the three explants used indicated that the endosperm-supported mature embryo was the most useful explant for plant regeneration in triticale.

7

Broadening the variability of quality traits in rapeseed through interspecific hybridization with an application of immature embryo culture

63%

Niemann J., Wojciechowski A., Janowicz J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2012

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tom 93

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nr 2

8

Interspecific hybridization between Cucumis sativus and wild Cucumis species through embryo culture

63%

Skalova D., Gasmanova N., Lebeda A., Navratilova B.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

9

Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

63%

Sun C.-Z., Li Y., Zhang S.-N., Zheng J.-S.

Acta Societatis Botanicorum Poloniae

|

2014

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tom 83

|

nr 1

Intergeneric hybrids between non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino; 2n = 4x = 40) and radish (Raphanus sativus L.; 2n = 4x = 36) were obtained through ovary culture and embryo rescue. Some hybrid embryos (0.11 per ovary) were produced, but only 4 of them germinated. As most hybrid embryos failed to develop into plantlets directly, plants were regenerated by inducing shoots on the cultured cotyledon and inducing roots on the root induction medium. All hybrid plants were morphologically uniform. They resembled the non-heading Chinese cabbage in the long-lived habit, the plant status, the vernalization requirement and the petiole color, while the petiole shape, leaf venation pattern and flowers were more similar to those of radish. Upon examination of the flowers, these were found to have normal pistil, but rudimentary anthers with non-functional pollen grains. The somatic chromosome number of F1 plants was 38. Analysis of SSR banding patterns provided additional confirmation of hybridity.

10

Somatic embryogenesis in zygotic embryo culture of Trifolium nigrescens Viv.

63%

Pilarska M., Konieczny R., Bohdanowicz J., Swierczynska J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

|

nr 1

11

In vitro seed germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer

63%

Kaveri S., Rao S.

International Letters of Natural Sciences

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2015

|

tom 48

In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA3, H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA3 and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

12

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis x S. cereale amphiploids with 2x and 4x S. cereale

63%

Wojciechowska B., Pudelska H.

Journal of Applied Genetics

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2005

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tom 46

|

nr 2

Amphiploids (2n = 6x = 42) of Ae. kotschyi and Ae. biuncialis with self-compatible S. cereale were produced from F₁ sterile hybrids (2n = 3x = 21) through colchicine treatment and callus tissue regeneration. The amphiploids resembled the F₁ plants in overall morphology, but were larger in all respects and self-fertile. The spikelets consisted mostly of 3 well-developed florets. Selfed seeds were obtained from some colchicine-doubled sectors and callus regenerates. Most of the produced seeds were well developed. Backcrosses between amphiploids and rye (2x and 4x) resulted in obtaining (Ae. biuncialis x S. cereale amphiploid) x S. cereale hybrids via embryo culture. The BC₁ plants (2n = 4x = 28 and 2n = 5x = 35, respectively) were phenotypically intermediate between the parents and vigorous in vegetative growth. Some seeds were obtained only from the 35-chromosome BC₁ hybrids.

13

The comparison of two different embryo culture methods in the course of in vitro fertilization program

51%

Marianowski P., Szymusik I., Grzechocinska B., Cyganek A.

Folia Histochemica et Cytobiologica. Supplement

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2007

|

tom 45

|

nr 1

115-117

14

Hybrids of tetraploid Aegilops sp. with Secale cereale

51%

Wojciechowska B.

Journal of Applied Genetics

|

1996

|

tom 37A

15

The effect of carbohydrate source on the development of Brassica napus L. immature embryos in vitro

51%

Slesak H., Przywara L.

Acta Biologica Cracoviensia. Series Botanica

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2003

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tom 45

|

nr 2

The effect of fructose, glucose, maltose and sucrose at various concentrations on Brassica napus embryos was studied. The morphogenetic reaction depended on the type and concentration of sugar in the medium. The frequency of developing embryos was highest on sucrose, followed by maltose and glucose. Fructose did not stimulate embryo growth. Spectrophotometry of autoclaved fructose showed an absorbance rise between 260-320 nm that could correspond to the formation of furfural derivatives. Autoclaving-induced toxicity probably inhibited embryo growth; heart-shaped stage embryos developed on filter-sterilized fructose. The frequency of developing embryos increased with sugar concentration, but normal embryogenesis occurred only on 1% sucrose and maltose; at higher concentrations callus and/or shoots were formed. On media with 6% sucrose and 12% maltose, shoots and somatic embryos were produced.

16

Some characteristics of flax embryo development in situ and in vitro

51%

Pretova A., Hajduch M., Obert B.

Acta Biologica Cracoviensia. Series Botanica

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2000

|

tom 42

|

nr 2

The article focuses on several aspects of flax embryo development in situ and in vitro. It describes the ultrastructural (plastid development), cytological and morphological events during zygotic embryo development and also some biochemical changes during the process, such as hormone level, accumulation of reserve materials, pigment quality and quantity, total protein analysis, and isozyme analysis. Next it characterizes the development of the zygotic embryo excised in different developmental stages and completing its development in vitro. Finally it follows the induction of direct and indirect somatic embryo formation in flax and the induction and formation of the androgenic embryo in anther cultures.

17

A mechanism for the transfer of resistance to Bipolaris sorokiniana [Sacc. in Sorok.] and Mayetiola destructor [Say] from tetraploid wheat [Triticum durum L.] to triticale [xTriticosecale Wittmack]

51%

Rogalska S. M., Barnett R. D., Soffes-Blount A. R.

Prace Komisji Nauk Rolniczych i Komisji Nauk Leśnych. Poznańskie Towarzystwo Przyjaciół Nauk

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1999

|

tom 87

18

Changes in the resin ducts structure of hypocotyl-epicotyl explants cultured on MS medium with IBA and kinetin

45%

Miszta A.

Acta Biologica Silesiana

|

1993

|

tom 24

36-45

19

Ocena efektywnosci kultur in vitro izolowanych zarodkow i zalazkow w otrzymywaniu mieszancow oddalonych w rodzaju Brassica

38%

Springer B., Wojciechowski A.

Rośliny Oleiste - Oilseed Crops

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2006

|

tom 27

|

nr 2

187-196

20

Evaluation of plant regeneration ability in culture of immature embryos of different Arabidopsis thaliana [L.] HEYNH. genotypes

38%

Gaj M. D., Ledwon U., Gaj M.

Acta Biologica Silesiana

|

1997

|

tom 31

66-73

Ograniczanie wyników

Triploids in cucumber: V. The effect of the homo- and heterozygous states on some traits of triploids grown in the field and under cover

Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

Methods of embryo scoring in in vitro fertilization

Influence of genotype and medium on culture of immature zygotic embryos of Cucumis sativus L. and Cucumis melo L.

Evaluation of the developmental toxicity of gentamicin using postimplantation rat whole embryo culture

A comparison of callus induction and plant regeneration from different embryo explants of triticale [x Triticosecale Wittmack]

Broadening the variability of quality traits in rapeseed through interspecific hybridization with an application of immature embryo culture

Interspecific hybridization between Cucumis sativus and wild Cucumis species through embryo culture

Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

Somatic embryogenesis in zygotic embryo culture of Trifolium nigrescens Viv.

In vitro seed germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis x S. cereale amphiploids with 2x and 4x S. cereale

The comparison of two different embryo culture methods in the course of in vitro fertilization program

Hybrids of tetraploid Aegilops sp. with Secale cereale

The effect of carbohydrate source on the development of Brassica napus L. immature embryos in vitro

Some characteristics of flax embryo development in situ and in vitro

A mechanism for the transfer of resistance to Bipolaris sorokiniana [Sacc. in Sorok.] and Mayetiola destructor [Say] from tetraploid wheat [Triticum durum L.] to triticale [xTriticosecale Wittmack]

Changes in the resin ducts structure of hypocotyl-epicotyl explants cultured on MS medium with IBA and kinetin

Ocena efektywnosci kultur in vitro izolowanych zarodkow i zalazkow w otrzymywaniu mieszancow oddalonych w rodzaju Brassica

Evaluation of plant regeneration ability in culture of immature embryos of different Arabidopsis thaliana [L.] HEYNH. genotypes