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The combination of SEM and autoclave methods gave a clear three-dimensional demonstration of integumental elastic fibres in small densely-haired mammals. The specific organisation of a fine and spongy elastic network was characterised by uniformly thin elastic fibres which were homogeneously distributed between both hair follicle types throughout the whole dermis. All the hair follicles were connected with each other by elastic fibres along their complete intradermal length. The advantage of such a specific elastic dermis construction is that all hair follicles can be moved together and simultaneously along the entire body, so that a better and rapid insulation is achieved after erection of the hair follicles during very low temperatures.
A microscopic study was carried out on 10 specimens of visceral and superior mesenteric arteries and their branches, taken from domestic pigs. Some thickenings in the middle tunic of the visceral artery and its main branches were observed. The thickenings appeared at intervals of about 2 cm and were even several centimetres long. The structure of the thickenings was similar. Besides numerous circularly arranged elastic fibres, it contained clusters of modified myocytes located under the stratifying internal elastic membrane. In cross-sections, the myocytes, lying perpendicularly to the proper muscle layer, looked like epithelial cells, and due to that, they were defined as epithelial muscle cells. They formed a distinct ring around the vessels (superior mesenteric artery). They may play the regulatory function in relation to the circularly arranged myocytes.
The aim of the study was to show the changes in the great saphenous vein (GSV) wall at consecutive stages in the development of chronic vein disease (CVD) in patients qualified for a surgical procedure after physical examination and Doppler ultrasonography. Four groups of patients were formed (C2, C3, C4 and C5/6) according to clinical stage of the CEAP classification (C — clinical signs, E — aetiopathology, A — anatomy and P —pathophysiology). After the surgical procedure for removal of the varicose GSVs, 40 segments were harvested from their proximal parts near the saphenofemoral junction, 10 segments for each CEAP group. The veins were sectioned transversally and stained with the resorcin-fuchsin and AZAN method to visualise the elastic end collagen fibres. Afterwards the specimens were analysed under an optical microscope and photographed. As the GSV is an elastic vessel and its wall is divided into three zones, namely the internal layer (intima), the medial layer (media) and the external layer (adventitia), we found a proliferation of the connective tissue among the smooth muscle cells inside the internal and medial layers at consecutive stages of CVD. The later stages of CVD also revealed a larger number of the elastic and collagen fibres inside the intima and media and a looser arrangement of the smooth muscle cells of the media in the GSV wall.
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