Wyniki wyszukiwania

1

Response to gonadotropic stimulation of cultuerd rat luteal cells isolated from corpora lutea of early pregnancy. Cytochemical and biochemical studies

100%

Gregoraszczuk E., Wojtusiak A.

Folia Histochemica et Cytobiologica

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1985

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tom 23

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nr 4

2

The expression of short form of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT-PCR

100%

Bogacka I., Przala J., Siawrys G., Kaminski T., Smolinska N.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 3

The study was conducted to determine gene expression of short form of leptin receptor (OB-Rs) using real time RT-PCR in distinct tissues of the central nervous system (medial basal hypothalamus, preoptic area, stalk median eminence), pituitary and reproductive tract (corpus luteum, ovarian stroma, endometrium, myometrium, and trophoblast) in pigs during luteal phase of the cycle and early gestation. The expression of OB-Rs mRNA in SME did not differ between analyzed stages of the cycle and pregnancy. In anterior pituitary, transcript levels were almost identical in mid- and late-luteal periods, but significantly decreased on 30-32 day of gestation when compared with day 14-16. In posterior pituitary, significantly higher expression was observed in two periods of pregnancy when compared with two stages of luteal phase. In corpus luteum the lowest expression was observed during days 10-12 of the cycle, whereas markedly higher levels were detected in late-luteal stage and gestation. In ovarian stroma the expression of Ob-Rs mRNA was markedly diminished during days 14-16 of the cycle when compared with days: 10-12 of the cycle and 30-32 of pregnancy. The expression of Ob-Rs mRNA in endometrium and myometrium reached the lowest levels on 30-32 day of pregnancy in comparison with earlier stage, 14-16 day. Summarizing, the expression of the short form of leptin receptor mRNA was found in majority of tested tissues including hypothalamus, pituitary and reproductive tract and their levels fluctuated depending on the phase (mid- and late-luteal) of the cycle and the day of pregnancy (early and late stage of implantation).

3

In vitro contractile activity of porcine myometrium during luteolysis and early pregnancy: effect of oxytocin and progesterone

84%

Kurowicka B., Franczak A., Oponowicz A., Kotwica G.

Reproductive Biology

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2005

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tom 05

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nr 2

4

Distributions of PCNA and Cas-3 in rat uterus during early pregnancy

84%

Oner H., Oner J., Demir R.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 1

71-77

5

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Meat quality of fattening pigs born from sows fed different diets during early pregnancy

84%

Rekiel A., Wiecek J., Bartosik J., Kuczynska B.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2016

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tom 55[2]

Meatquality of fattening pigs born from sows fed different diets during early pregnancy. The aim of the study was to determine the pork quality of fattening pigs (Polish Landrace ´Polish Large White ´Duroc) originated from sows fed during early pregnancy (41 to 70 days) with diets differing in quantity and quality (LD1 vs LK sows – 130% of recommendations, LD2 sows – energy supplement). Thirty-six weaners from LK, LD1 and LD2 sows were selected for fattening and assigned to groups K, D1 and D2 (12 animals per group). Fatteners were fed in 3 phases and kept under standardized environmental conditions (individual pens, constant access to water). Better growth rate in the offspring was obtained as a result of temporarily more intense feeding of pregnant sows with increased daily rations. Modification of the sow diets had no appreciable effect on the slaughter traits of the fatteners postmortem, which shows that there are no contraindications for temporary changes in the feeding of pregnant sows. Good and uniform meatiness of the fatteners in the control and experimental groups was obtained. Chemical composition and physical characteristics of the meat were similar among the groups and the meat was of good quality. Statistically significant differences between the groups were found for several traits: crude ash content, a* colour value, and the proportion of C17:1 and C18:2 n-6 acids.

6

Characterization of the immune response generated in the placenta of cattle experimentally infected with Neospora caninum in early gestation

84%

Maley S., Buxton D., Macaldowie C., Anderson I., Wright S., Bartley P., Esteban-Redondo I., Hamilton C., Storset A., Innes E.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

7

Proteome of spleen CD4 lymphocytes in mouse preimplantation pregnancy

84%

Chelmonska-Soyta A., Ozgo M., Lepczynski A., Herosimczyk A., Buska-Pisarek K., Kedzierska A., Nowak D., Mazur A. J.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 5

8

Effect of short-lasting undernutrition of gilts during peri-conceptional period on biochemical and haematological parameters in blood plasma during peri-implantation period

84%

Franczak A., Zmijewska A., Zglejc K., Dziekonski M., Waszkiewicz E., Okrasa S., Sobotka W., Kotwica G.

Journal of Elementology

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2016

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tom 21

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nr 1

In gilts, the period of early pregnancy occurring from the time of fertilization to the beginning of implantation is sensitive to any environmental disruptions, including an unbalanced diet of a future mother. Previously, we found that due to the undernutrition in gilts during this period, the endocrine intrauterine microenvironment and DNA methylation in the uterus have been changed. These distortions may diminish the success of pregnancy. In this study we focused on the influence of a restricted diet used in gilts during the first days of pregnancy on their biochemical and haematological parameters in peripheral blood. The applied restrictive diet vs. normal diet covered only 70% of the nutritional demands of early pregnant gilts. Normal (n = 4 gilts) or restrictive (n = 5 gilts) diets were used from the day of the first signs of the estrus until day 9 of pregnancy and biochemical and haematological parameters in blood plasma were determined during peri-implantation period, e.g. on days 15 to16 of pregnancy. In restrictive vs. normal fed gilts significantly lower plasma phosphorus, calcium and total cholesterol as well as the tendency to increasing concentrations of triglicerydes and asparate aminotranserase were found. Haematological parameters did not differ between the studied gilts. Thus, it seems that the availability of nutritional factors became suboptimal in restrictively fed early pregnant gilts. Even short-lasting undernutrition of females during the peri-conceptional period may cause a disruption of biochemical homeostasis during the peri-implantation period and probably affect the success of pregnancy.

9

Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy

84%

Demir R.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 3

10

Effect of estradiol, progesterone and interleukin-1beta on prostaglandin E2 and F2 secretion by porcine corpora lutea in vitro during the periimplantation period

84%

Wasielak M., Kaminska K., Bogacki M.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 04B

This study examines the influence of ovarian steroids and interleukin-1β on prostaglandin E2 (PGE2) and F2α (PGF2α) synthesis and secretion from corpora lutea (CL) slices obtained from ovaries of gilts on days 12-14 of pregnancy and days 12-14 of the estrous cycle. For this study the authors used a surgically-generated model in which one of the uterine horns was cut transversely and a in separated part of this horn embryos did not develop. Collected corpora lutea were cut into slices and treated for 6 h with estradiol (E2) (100 nM/ml), progesterone (P4) (50 nM/ml) and interleukin-1β (IL-1β) (2.5 ng/ml). Results indicated that P4 increased PGE2 synthesis in CL slices originating from the ovary ipsilateral to the uterine horn with the developing embryos. P4 did not influence PGF2α synthesis in cultured CL slices from the ovaries of pregnant gilts regardless of embryos present in the uterine horn. In contrast to the effect of P4, E2 increased PGF2α synthesis in cultured CL slices from the ovary ipsilateral to the uterine horn with the developing embryos. E2 had no effect on PGF2α content in CL slices from the contralateral ovary of pregnant gilts and CL slices from both ovaries of cyclic gilts but increased PGF2α release from CL slices from the ipsilateral ovary of pregnant gilts. IL-1β markedly increased PGE2 release from cultured CL slices from the ovary contralateral to the uterine horn with the developing embryos from pregnant gilts and cyclic gilts regardless of CL origin.

11

In vitro effects of luteinizing hormone, progesterone and oestradiol-17beta on leptin gene expression and leptin secretion by porcine luteal cells obtained in early pregnancy

67%

Siawrys G., Smolinska N.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 4

12

The effect of intravaginal application of estradiol and progesterone on porcine embryo development

67%

Chlopek J., Gilun P., Tabecka-Lonczynska A., Koziorowski M., Stefanczyk-Krzymowska S.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 4

287-293

This study was designed to check whether intravaginal application of estradiol and progesterone on chosen days of early pregnancy affects the development and survival of porcine embryos. Crossbred gilts were assigned to control (C-land C-2) or experimental (T-l and T-2) groups. Systemic blood samples were collected in groups C-l and T-l on days 3-18 of pregnancy. Gilts of control groups were supplied on days 2-6 and 12-17 of pregnancy with intravaginal cocoa butter suppositories. Gilts of experimental groups were intravaginally supplied on days 2-6 of pregnancy with estradiol (0.2 mg) to improve uterine blood supply and on days 12-17 of pregnancy gilts were supplied with progesterone (0.25 mg) to stimulate the secretory function of the endometrium during the periimplantation period. Systemic concentration of progesterone was elevated on days 3-6 and of estradiol on days 4, 5 and 10 of pregnancy in the experimental groups in comparison with the control gilts. The weight of the ovary and number of corpora lutea were similar in the control and experimental groups, but the number of follicles over 3 mm in diameter was higher in the experimental groups than in control (P < 0.01) on day 30 of pregnancy. The mean ovulation rate, total number of embryos and rate of embryo survival on day 30 of pregnancy were similar in the control and experimental groups. However, the weight, crown-rump length and width of embryos were greater in the experimental groups than in control (P < 0.001). The results demonstrated that the intravaginal application of estradiol and progesterone on precisely chosen days of early pregnancy stimulated embryo development. This study demonstrated that the timing of hormone administration must comply with the physiological effect of estradiol and progesterone on uterine blood supply to ensure embryo development and survival.

13

Contractile effect of PGF2alpha and PGE2 on isolated branches of uterine and ovarian artery in different days of estrous cycle and early pregnancy in pigs

67%

Skipor J., Pikulinska M., Stefanczyk-Krzymowska S.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 4

The contractile effects of PGF₂α (3 × 10⁻⁶ to 10⁻⁴ M) and PGE₂ (10⁻⁷ to 10⁻⁵ M) were examined on isolated branches of ovarian artery (OA) and extramyometrial branches of uterine artery (UA) collected from pigs in the luteal (day 10-12) and follicular phase (day 17-20) of the estrous cycle, and during early pregnancy (day 10-12). Strong contraction was demonstrated in both arteries during all investigated periods in response to PGF2α, which was significantly higher (P < 0.01) than to PGE₂, being negligible in the follicular phase. In UA, the effective dose of PGF₂α (ED50) amounted 7.9 × 10⁻⁶ M and 6.3 × 10⁻⁶ M in the luteal and follicular phase, and 5.0 × 10⁻⁶ M in early pregnancy. ED50 for PGE₂ reached 5.0 × 10⁻⁷ M in the luteal phase, and 4.1 × 10⁻⁷ M in early pregnancy. For both prostaglandins, the contraction was much stronger (P < 0.01) in OA than in UA branches. In OA, the ED50 for PGF₂α was 1.2 × 10⁻⁵ M in the luteal phase and was significantly higher (P < 0.05) than in the follicular phase (3.1 × 10⁻⁶ M) and early pregnancy (2.7 × 10⁻⁶ M). ED50 for PGE2 amounted 7.3 × 10⁻⁷ M in the luteal phase and 1.7 × 10⁻⁷ M in early pregnancy. Studies showed the influence of the estrous cycle and early pregnancy on OA branches sensitivity to the contractile effect of PGF₂α and the lack of this effect on UA branches, and the influence of the estrous cycle on UA and OA branch contraction in response to PGE2.

14

In vitro effect of peroxisome proliferator activated receptor (PPAR) ligands on prostaglandin E2 synthesis and secretion by porcine endometrium during the estrous cycle and early pregnancy

67%

Bogacka I., Bogacki M., Gaglewska M., Kurzynska A., Wasielak M.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 1

15

Expression and cellular distribution of NADPH-diaphorase and nitric oxide synthases in the porcine uterus during early pregnancy

67%

Andronowska A., Chrusciel M.

Folia Histochemica et Cytobiologica

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2007

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tom 45

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nr 4

375-380

16

Histochemical localization of NADPH-d-positive structures in bovine uterine and ovarian arteries during the oestrous cycle and early pregnancy

67%

Jaroszewski J. J., Barszczewska B., Wojtkiewicz J., Majewski M., Timmermans J.-P.

Folia Histochemica et Cytobiologica

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2002

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tom 40

|

nr 2

17

Leptin gene and protein expression in the trophoblast and uterine tissues during early pregnancy and the oestrous cycle of pigs

67%

Smolinska N., Siawrys G., Kaminski T., Przala J.

Journal of Physiology and Pharmacology

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2007

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tom 58

|

nr 3

Leptin is a 16-kDa protein hormone encoded by the obese (ob) gene and acts on receptors in the hypothalamus to regulate food intake and energy balance. The identification of leptin and its receptor mRNAs and proteins in human and mouse endometrium and placental trophoblast has attracted attention to the potential role of leptin in implantation. Thus, the aim of this study was to compare the expression levels of porcine leptin mRNA and protein in endometrium and myometrium during mid- and late-luteal phases of the oestrous cycle (days 10 - 12 and 14 - 16) as well as during two stages of pregnancy respondent to the beginning (days 14 - 16) and the end (days 30 - 32) of the implantation process, and in trophoblast during both periods of pregnancy. Leptin gene and protein expression in myometrium, and leptin mRNA expression in endometrium was more pronounced in the mid- and late-luteal phases of the cycle in comparison to studied periods of pregnancy, whereas leptin protein concentration in endometrium was either enhanced on days 30 - 32 of pregnancy in relation to days 14 - 16 of the cycle or there were no changes between pregnancy and luteal phase of the cycle. On days 30 - 32 of pregnancy, expression of the leptin gene in the endometrium, and of the leptin gene and protein in the myometrium was more pronounced in comparison to the earlier stage of pregnancy. Moreover, leptin gene expression in porcine trophoblast increased during the beginning of the implantation process compared to days 30 - 32 of pregnancy, while the protein concentration decreased on days 14 - 16 of pregnancy. In conclusion, the finding of leptin gene and protein expression in porcine endometrium, myometrium and trophoblast indicates that locally synthesised leptin can participate in the control of pig reproduction. The fluctuation of the hormone concentration during pregnancy and changes in its level between pregnancy and the oestrous cycle may indicate leptin's involvement in the implantation process.

18

Relative transcript abundance of oxytocin receptor gene in porcine uterus during luteolysis and early pregnancy

67%

Oponowicz A., Franczak A., Kurowicka B., Kotwica G.

Journal of Applied Genetics

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2006

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tom 47

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nr 4

The aim of the present study was to investigate transcript localization of the oxytocin receptor (OTR) gene in different cells of the porcine uterus during luteolysis and early pregnancy (days 14-16) using in situ hybridization (ISH). OTR mRNA was localized in the uterine luminal epithelium (LEC), glandular epithelium (GEC), stromal cells (SC) of the endometrium, in the longitudinal muscle layer (LM) and circular muscle layer (CM) of the myometrium. The OTR transcript was quantified by optical density units of silver grains. The OTR transcript levels in the endometrium and myometrium were statistically higher during luteolysis than during early pregnancy (P < 0.05). Besides, during luteolysis, the mRNA level was higher in the LEC, GEC of the endometrium and LM of the myometrium compared to that observed in the SC of endometrium and CM of the myometrium, respectively (P < 0.05). In summary: 1) the level of OTR mRNA in uterine tissues is higher during luteolysis compared to early pregnancy, 2) the OTR transcript level in endometrial cells did not correspond to the sensitivity of these cells to oxytocin (ОТ), 3) the myometrial expression of the OTR gene is appropriate to control contractile activity and secretion of PG during luteolysis.

19

Praktyczne zastosowanie oznaczeń poziomu progesteronu w mleku krów. I. Rozpoznanie wczesnej ciąży

59%

Janowski T.

Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria

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1988

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tom 18

20

Mechanisms ensuring optimal conditions of implantation and embryo development in the pig

59%

Przala J., Gregoraszczuk E. L., Kotwica G., Stefanczyk-Krzymowska S., Ziecik A. J., Blitek A., Ptak A., Rak A., Wojtowicz A., Kaminski T., Siawrys G., Smolinska N., Franczak A., Kurowicka B., Oponowicz A., Wasowska B., Chlopek J., Kowalczyk A. E., Kaczmarek M. M., Waclawik A.

Reproductive Biology

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2006

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tom 06

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nr Suppl.1

Ograniczanie wyników

Response to gonadotropic stimulation of cultuerd rat luteal cells isolated from corpora lutea of early pregnancy. Cytochemical and biochemical studies

The expression of short form of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT-PCR

In vitro contractile activity of porcine myometrium during luteolysis and early pregnancy: effect of oxytocin and progesterone

Distributions of PCNA and Cas-3 in rat uterus during early pregnancy

Meat quality of fattening pigs born from sows fed different diets during early pregnancy

Characterization of the immune response generated in the placenta of cattle experimentally infected with Neospora caninum in early gestation

Proteome of spleen CD4 lymphocytes in mouse preimplantation pregnancy

Effect of short-lasting undernutrition of gilts during peri-conceptional period on biochemical and haematological parameters in blood plasma during peri-implantation period

Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy

Effect of estradiol, progesterone and interleukin-1beta on prostaglandin E2 and F2 secretion by porcine corpora lutea in vitro during the periimplantation period

In vitro effects of luteinizing hormone, progesterone and oestradiol-17beta on leptin gene expression and leptin secretion by porcine luteal cells obtained in early pregnancy

The effect of intravaginal application of estradiol and progesterone on porcine embryo development

Contractile effect of PGF2alpha and PGE2 on isolated branches of uterine and ovarian artery in different days of estrous cycle and early pregnancy in pigs

In vitro effect of peroxisome proliferator activated receptor (PPAR) ligands on prostaglandin E2 synthesis and secretion by porcine endometrium during the estrous cycle and early pregnancy

Expression and cellular distribution of NADPH-diaphorase and nitric oxide synthases in the porcine uterus during early pregnancy

Histochemical localization of NADPH-d-positive structures in bovine uterine and ovarian arteries during the oestrous cycle and early pregnancy

Leptin gene and protein expression in the trophoblast and uterine tissues during early pregnancy and the oestrous cycle of pigs

Relative transcript abundance of oxytocin receptor gene in porcine uterus during luteolysis and early pregnancy

Praktyczne zastosowanie oznaczeń poziomu progesteronu w mleku krów. I. Rozpoznanie wczesnej ciąży

Mechanisms ensuring optimal conditions of implantation and embryo development in the pig