Wyniki wyszukiwania

1

Australian Hymenolepididae parasitizing birds. VI. Sobolevicanthus dobsoni sp. n. found in the wood duck Chenonetta jubata (Latham)

100%

Czaplinski B.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1989

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tom 37

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nr 4-6

2

Gastric and duodenal ulcer and concomitant diseases among rural population

100%

Schabowski J. P.

Annals of Agricultural and Environmental Medicine

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1994

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tom 01

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nr 2

3

The role of tyrosine kinase in prostaglandin E2 and vanadate-evoked contractions in rabbit duodenum in vitro

100%

Grasa L., Arruebo M. P., Plaza M. A., Murillo M. D.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

Prostaglandin E2 (PGE2) can interact with at least four cell surface receptors (EP1-EP4) in smooth muscle, which evokes a variety of intracellular responses depending on the G protein to which the cell surface receptors are coupled. The activation of G protein-coupled receptors and receptor tyrosine kinases can lead to the phosphorylation of tyrosine residues of various cellular proteins. The aim of this study was to examine the role of tyrosine phosphorylation in PGE2, vanadate and carbachol-evoked contractions. PGE2, vanadate, and carbachol induced contractile motor responses in the longitudinal smooth muscle of rabbit duodenum. PGE2-evoked contractions decreased in the presence of genistein or tyrphostin B44. PGE2-evoked contractions increased in the presence of vanadate. Vanadate-evoked contractions decreased in the presence of genistein. In contrast, tyrphostin 47 increased the vanadate-evoked contractions. Vanadate-evoked contractions were reduced in the presence of Ca2+-free solutions, verapamil, or indomethacin. U-73122 decreased PGE2-evoked contractions. Carbachol-evoked contractions decreased in the presence of genistein, tyrphostin B44 or tyrphostin 47. Our results suggest that PGE2, vanadate or carbachol-evoked contractions are mediated by protein tyrosine phosphorylation. Protein tyrosine phosphorylation might cause an increase in calcium influx through voltage-dependent channels and the release of prostaglandins in the longitudinal smooth muscle of the rabbit duodenum.

4

Expression of vanilloid receptor-1 in the duodenum of the capsaicin treated rat

100%

Zik B., Ozguden C., Tutuncu S., Ilhan T.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 1

The aim of the presented investigation was to identify different vanilloid receptor-1 (VR1) immunoreactivity after application of low dose of capsaicin (0.5 mg/kg) for a prolonged period in the rat's duodenum. Paraffin-embedded sections were processed for standard immunohistochemistry by the labelled streptavidin-biotin technique. The VR1 localisations were identified on the epithelial layers of the villi, in the Brunner's glands, smooth muscles layer, and the neurons of the myenteric plexus of the duodenum. While VR1 immunoreactivity was identified in small quantities in the control group, VR1 expression was strong both in the experimental and in the vehicle treated group. These results indicate that prolonged administration of a low dose of capsaicin may not be sufficient to stimulate VR1. Also the vehicle additives, Tween 80 and 10% ethanol, which are used to solubilize capsaicin, may activate the protection mechanism in the mucosa epithelium and stimulate the capsaicin sensitive afferent neurons by VR1 to increase mucus secretion.

5

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Role of nitric oxide in gastroduodenal alkaline secretion

100%

Bilski J., Konturek S. J.

Journal of Physiology and Pharmacology

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1994

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tom 45

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nr 4

6

CCK1 central receptor antagonist prevented the intestinal stress symptoms in sheep

84%

Kania B. F., Matczuk J., Romanowicz K., Bueno L., Fioramonti J., Kania K.

Polish Journal of Veterinary Sciences

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2002

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tom 05

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nr 1

7

Visualisation of diverticula of the upper part of the alimentary tract: comparison of roentgenologic and endoscopic techniques [Erratum]

84%

Burdan F., Rozylo-Kalinowska I., Juskiewicz W., Maciejewski R., Wallner G., Zgodzinski W., Zinkiewicz K., Zlomaniec J., Dabrowski A.

Folia Morphologica

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2002

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tom 61

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nr 1

297-301

8

Pathological lesions in European bison [Bison bonasus] with infestation by Ashworthius sidemi [Nematoda, Trichostrongylidae]

84%

Osinska B., Demiaszkiewicz A. W., Lachowicz J.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 1

63-67

Asworthius sidemi Schulz, 1933 is a blood sucking gastrointestinal nematode, primarily typical for Asiatic deer. It was found for the first time in Poland in European bison in 1997. To estimate the level of invasion of A. sidemi and histopathological changes connected with the presence of the parasite in the years 2004-2007 parasitological and histopathological examinations of 54 European bison from Białowieża Forest were carried out. Parasitological examination was carried out by the sedimentation method and A. sidemi were diagnosed under a binocular microscope. Samples for histological examination were collected from the abomasum and duodenum walls as well as from regional lymph nodes. Tissue samples were then fixed with 10% buffered formalin, embedded in paraffin, cut in to 5 μm thick sections and stained with hematoxylin and eosin (H&E). Parasitological examinations showed the presence of fourth stage larvae and juvenile forms of A. sidemi. The maximal intensity of invasion rose systematically from 4470 A. sidemi nematodes in 2004/2005 to 44310 in 2006/2007. Histopathological examinations showed infiltrations of inflammatory cells in the walls of abomasa and duodena at various levels of intensity (mainly lymphoid cells and eosinophils), hyperemiae, oedemae and lesions of mucosa and proliferation of lymphatic follicles. In individual cases of dysplasia of epithelial cells, atrophy or hyperplasia of glands and the presence of parasites in the lumen or walls of the abomasum/duodenum were observed. In one case, parasitic nodules were found. In regional lymph nodes proliferation of lymphatic follicles, presence of eosinophils and desolation of reproduction centers were observed. Intensification of histopathological changes was connected to a considerable degree with the developmental stage of A. sidemi as shown by parasitological examination.

9

The effect of soya protein supplement enriched with three amino acids on duodenal flow of soluble nitrogen fractions and amino acids in dairy cows

84%

Krizova L., Richter M., Trinacty J., Sterc J., Hadrova S.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 3

345-356

10

Microbiota of human gastrointestinal tract

84%

Lewandowska M.

Zeszyty Naukowe. Politechnika Łódzka. Chemia Spożywcza i Biotechnologia

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2010

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tom 74

11

A downregulation of nNOS is associated to dysmotility evoked by lipopolysaccharide in rabbit duodenum

84%

Grasa L., Arruebo M. P., Plaza M. A., Murillo M. D.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 3

511-524

Alterations in gastrointestinal motility have been reported in response to endotoxin. The effects of lipopolysaccharide (LPS) on motility have been attributed to several substances, including prostaglandins and nitric oxide. The aim of this study was to investigate the expression and the contribution of NOS and COX enzymes to the local effect of LPS on ACh-evoked contractions in rabbit duodenum. The ACh evoked contractions were inhibited by LPS in longitudinal and circular muscles of duodenum. L-NNA, aminoguanidine, ODQ, indomethacin, and NS-398 but not NPLA antagonized the inhibitory effect of LPS. Western blot analysis showed protein bands of 155, 130, 70 and 72 kDa for nNOS, iNOS, COX-1 and COX-2 respectively in rabbit duodenum. All of these isoforms were expressed constitutively and only the nNOS was reduced in the presence of LPS. Expression of nNOS, iNOS, COX-1 and COX-2 was detected by inmunohistochemistry in the smooth muscle layers and in the neurons of the myenteric ganglia of rabbit duodenum. In conclusion, LPS locally administered reduces the contractility of rabbit duodenum and a downregulation of nNOS is associated to this effect. The iNOS, COX-1 and COX-2 were expressed constitutively but their expression was not modified by LPS.

12

Role of cholecystokinin in postprandial and vagally stimulated duodenal and gallbladder motility in dogs

84%

Thor P., Laskiewicz J., Maczka M., Konturek S. J.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

This study was designed to determine the role of cholecystokinin (CCK) in postprandial motility pattern of the duodenum and gallbladder (GB) in conscious dogs provided with chronic duodenal electrodes for recording of myoelectric activity and GB fistulas for measurement of intraluminal pressure and volume of GB and to calculate the GB motility index (MI) and GB emptying rate. During naturally occuring activity front (phase III MMC) in the duodenum there was significant increase in the MI of GB accompanied by about 20-30% reduction in the GB volume. These changes in duodenal and GB motility pattern could be duplicated by i. v. motilin. Feeding abolished the appearence of spontaneous activity front in the duodenum and greatly increased motility of GB while reducing its volume. Administration of CCK receptor antagonists in fed dogs failed to affect the motility changes induced by meal in the duodenum but abolished these of the GB. Vagal cholinergic stimulation with insulin, 2DG or urecholine caused similar effects to that induced by food i. e. increased duodenal spike activity, abolished phase III of the MMC, decreased GB volume and increased GB motility. Pretreatment with CCK antagonists did not affect significantly duodenal spike activity or GB motility but significantly increased the GB volume. Atropine 125 µg/kg) blocked almost completely spontaneous activity front in the duodenum and accompanying alterations in the motiliti and volume of GB. We conclude that CCK contributes to the MMC related alterations in the GB motor activity and is essential in cholinergic stimulation induced of the GB emtying but not in vagally induced duodenal and GB motility.

13

Cholecystokinin octapeptide and cerulein inhibit ovine duodenal motility in a dose- and region-specific manner

84%

Romanski K. W.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

251-256

The aim of this study was to examine the influence of cholecystokinin (CCK)-octapeptide (OP) and its amphibian analogue cerulein infusions on duodenal myoelectric and motor activities, as well as to compare the effects of CCK peptides on duodenal bulb and duodenal motility in non-fasted conscious rams. Five rams underwent implantation of bipolar platinum electrodes to the duodenal bulb, and distal duodenum, as well as a strain gauge force transducer near the duodenal electrode. During continuous myoelectrical and motor recordings, 0.15 M NaCl or CCK peptides were administered intravenously. Infusions of CCK-OP at doses of 5 and 50 ng/kg/min and infusions of cerulein at doses of 0.5 and 1.5 ng/kg/min were applied for 60 min and started 15 min after the onset of the duodenal phase 2b of the migrating motor complex. The higher infusion dose of the CCK-OP in the duodenal bulb triggered the strong inhibitory response within few minutes following the start of infusion while in the duodenum its inhibitory effect was shorter and arrived within 40-50 min following the onset of the infusion. The higher dose of cerulein evoked a reaction similar to CCK-OP response in the duodenal bulb while in the duodenum the clear inhibitory response arrived about 20 min earlier than after CCK-OP. A lower infusion dose of CCK peptides evoked less pronounced effects. It is concluded that CCK-OP inhibits ovine duodenal motility in a dose-and region-specific manner. This effect seems to be physiological.

14

The role of Ca2plus in the contractility of rabbit small intestine in vitro

84%

Grasa L., Rebollar E., Arruebo M. P., Plaza M. A., Murillo M. D.

Journal of Physiology and Pharmacology

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2004

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tom 55

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nr 3

This study evaluated the role of Ca2+ in spontaneous and ACh- and KCl-induced contractions in longitudinal and circular smooth muscle from rabbit small intestine in vitro. In the first experiment, the amplitude, frequency and tone of spontaneous contractions in longitudinal and circular smooth muscle of small intestine were determined and, in the second experiment, the ACh- and KCl-induced responses of longitudinal and circular smooth muscle were measured. Atropine and guanethidine reduced the amplitude and tone of contractions in longitudinal and circular muscle, but reduced the frequency of contractions in circular muscle, only. TTX attenuated the amplitude of contractions and decreased the tone of contractions in longitudinal muscle, but increased the tone in circular muscle. Ca2+-free solutions, verapamil, nifedipine and caffeine diminished the three parameters of spontaneous contractions. Thapsigargin and cyclopiazonic acid increased the amplitude and tone of contractions in ileum longitudinal muscle, only, and cyclopiazonic acid increased the amplitude of contractions in circular muscle. Ca2+-free solutions, verapamil, nifedipine, thapsigargin, cyclopiazonic acid, and caffeine diminished ACh- and KCl-induced contractions. Those results suggest that extracellular Ca2+ plays a role in spontaneous contractions, and extracellular and intracellular Ca2+ participate in the ACh- and KCl-induced contractions of rabbit small intestine.

15

Influence of ghrelin on gastric and duodenal growth and expression of digestive enzymes in young mature rats

84%

Warzecha Z., Dembinski A., Ceranowicz P., Dembinski M., Cieszkowski J., Konturek S. J., Polus A., Pawlik W. W., Kuwahara A., Kato I., Konturek P. C.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 3

Ghrelin, a nature ligand for the growth hormone secretagogue receptor (GHS-R), stimulates a release of growth hormone, prolactin and adrenocorticotropic hormone. Also, ghrelin increases food intake in adult rats and humans and exhibits gastroprotective effect against experimental ulcers induced by ethanol or stress. The aim of present study was to examine the influence of ghrelin administration on gastric and duodenal growth and expression of pepsin and enterokinase in young mature rats with intact or removed pituitary. Methods: Two week after sham operation or hypophysectomy, eight week old Wistar male rats were treated with saline (control) or ghrelin (4, 8 or 16 nmol/kg/dose) i.p. twice a day for 4 days. Expression of pepsin in the stomach and enterokinase in the duodenum was evaluated by real-time PCR. Results: In animals with intact pituitary, treatment with ghrelin increased food intake, body weight gain and serum level of growth hormone and insulin-like growth factor-1 (IGF-1). These effects were accompanied with stimulation of gastric and duodenal growth. It was recognized as the significant increase in gastric and duodenal weight and mucosal DNA synthesis. In both organs, ghrelin administered at the dose of 8 nmol/kg caused maximal growth-promoting effect. In contrast to these growth-promoting effects, administration of ghrelin reduced expression of mRNA for pepsin in the stomach and was without effect on expression of mRNA for enterokinase in the duodenum. Hypophysectomy alone lowered serum concentration of growth hormone under the detection limit and reduced serum level of IGF-1 by 90%. These effects were associated with reduction in daily food intake, body weight gain and gastroduodenal growth. In hypophysectomized rats, administration of ghrelin was without significant effect on food intake, body weight gain or growth of gastroduodenal mucosa. Also, serum concentration of growth hormone or IGF-1 was not affected by ghrelin administration in rats with removed pituitary. Conclusion: Administration of ghrelin stimulates gastric and duodenal growth in young mature rats with intact pituitary, but inhibits expression of mRNA for pepsin in the stomach. Growth hormone and insulin-like growth factor-1 play an essential role in growth-promoting effects of ghrelin in the stomach and duodenum.

16

Effect of hypodynamy on structure and enzymatic activity of duodenal enterocytes of Japanese quail

84%

Cigankova V., Zibrin M., Lenhardt L., Almasiova V., Holovska K., Skrobanek P.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 1

The effect of hypodynamy on the structure, ultrastructure, and enzymatic activity of the duodenal enterocytes of Japanese quail (Coturnix coturnix japonica) was studied. On the second day after hatching, 42 chicks were placed in individual slings suspended by a flexible metal device in such a manner that their legs could not touch the floor. Feed and water was provided ad libitum. The experimental animals were euthanized at 5, 7, 14, 21, 28, 35, and 42 d of age. The birds of control group were kept under standard conditions on the floor. Samples of the duodenum were routinely processed for a light microscope (LM) and also a transmission electron microscope (TEM), along with the determination for alkaline phosphatase (AP) activity. Morphological changes in enterocytes of chicks reared under hypodynamy from day 2 after hatching were observed on days 5 and 7 of age. LM showed that the apical ends of intestinal villi contained clumps of necrotizing enterocytes, which were more numerous compared to that of the control. TEM revealed a loss of microvilli and changes in both the nucleus and cytoplasm in these cells. Besides considerable damage to mitochondria, they exhibited also typical vesiculation of cytoplasm and damaged intercellular junctions. From day 14 to 42 of age morphological changes were less pronounced. On day 7 of age, the activity of AP in the microvilli of duodenal enterocytes of experimental chicks was increased in comparison with the control (P<0.001). On day 14 of the experiment, AP in the investigated structures showed only a slight increase (P<0.05), and on day 21 was also slightly increased (P<0.01). On days 28, 35, and 42 of age, no significant differences in the activity of AP were observed. Hypodynamy acted as a stress factor on Japanese quail, which was stronger at the beginning of the experiment. The animals gradually adapted to their respective conditions. Our results show that Japanese quail chicks are capable of feed consumption and feed conversion under conditions of hypodynamy.

17

Fatty acid flow to the duodenum of sheep fed diets supplemented with different types of fat

84%

Szumacher-Strabel M., Potkanski A., Kowalczyk J., Cieslak A., Czauderna M., Strzelecka G., Jedroszkowiak P.

Journal of Animal and Feed Sciences

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2003

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tom 12

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nr 2

18

Development and regulation of pancreatic juice secretion in cattle. State-of-the-art

84%

Zabielski R., Pierzynowski S. G.

Journal of Animal and Feed Sciences

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2001

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tom 10

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nr 1

19

A note on the effect of different amounts and types of fat on the fatty acid composition of duodenal digesta in sheep

84%

Potkanski A., Szumacher-Strabel M., Kowalczyk J., Cieslak A., Czauderna M.

Journal of Animal and Feed Sciences

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2001

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tom 10

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nr 4

20

Central nervous stimuli increase duodenal bicarbonate secretion by release of mucosal melatonin

84%

Sjoblom M., Flemstrom G.

Journal of Physiology and Pharmacology

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2001

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tom 52

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nr 4,1

A number of common diseases in humans, including gastroduodenal ulcer and irritable bowel syndrome, show circadian rhythms in pain and discomfort. The neurohormone melatonin is released from enterochromaffin cells in the intestinal mucosa and from the pineal gland but its role in gastrointestinal function is largely unknown. We have studied the involvement of melatonin in stimulation of the mucosa-protective alkaline secretion by the duodenal mucosa. A 12-mm segment of proximal duodenum with an intact blood supply was cannulated in situ in anesthetized rats and duodenal HCO3– secretion titrated by pH-stat. Duodenal close intra-arterial infusion of melatonin or the full agonist 2-iodo-N-butanoyl- 5-methoxytryptamine significantly increased the secretion and pretreatment with the melatonin (predominantly MT2-receptor specific) antagonist luzindole almost abolished the response. Intracerebroventricular (i.c.v.) infusion of the alpha1-adrenoceptor agonist phenylephrine (12.2 µmol kg-1·h-1 ) caused an up to fivefold increased in the alkaline secretion and the melatonin antagonist luzindole or cutting all peri-carotid nerves abolished the duodenal secretory response to i.c.v. phenylephrine. Peripheral melatonin thus stimulates duodenal mucosal HCO3 – secretion and endogenous melatonin, very likely released from mucosal enterochromaffin cells, is involved in mediating neural stimulation of the secretion.

Ograniczanie wyników

Australian Hymenolepididae parasitizing birds. VI. Sobolevicanthus dobsoni sp. n. found in the wood duck Chenonetta jubata (Latham)

Gastric and duodenal ulcer and concomitant diseases among rural population

The role of tyrosine kinase in prostaglandin E2 and vanadate-evoked contractions in rabbit duodenum in vitro

Expression of vanilloid receptor-1 in the duodenum of the capsaicin treated rat

Role of nitric oxide in gastroduodenal alkaline secretion

CCK1 central receptor antagonist prevented the intestinal stress symptoms in sheep

Visualisation of diverticula of the upper part of the alimentary tract: comparison of roentgenologic and endoscopic techniques [Erratum]

Pathological lesions in European bison [Bison bonasus] with infestation by Ashworthius sidemi [Nematoda, Trichostrongylidae]