The aim of the research was to modify the Quyang and Wu method for determination of selenomethionine level in selenium yeast. Modification concerned the application of capillary column for chromatographic separation and addition of the internal standard (undecanoic acid methyl ester) to the sample before analysis. The use of capillary column in analytical process significantly improved the separation of components into single, well — shaped (symmetrical) peaks, facilitating the quantitative measurements.
Przedmiotem badań było określenie wpływu drożdży selenowych Saccharomyces cerevisiae zastosowanych u 7-dniowych cieląt na nasilenie klinicznych objawów biegunki, liczebność flory bakteryjnej w ich kale, wybrane wskaźniki biochemiczne ich krwi, i przyrosty masy ciała. Drożdże podawane były z preparatem mlekozastępczym w dawce 67 mg/dobę/sztukę. Zastosowany preparat spowodował redukcję ilości patogennej flory bakteryjnej w kale badanych zwierząt oraz wzrost stężenia y-globulin w surowicy ich krwi. Odnotowano większe przyrosty masy ciała u osobników otrzymujących preparat.
The objective of the study was to evaluate the efficacy of two selenium sources (sodium selenite and Se enriched yeast) in terms of its concentration in the liver, heart, kidneys and skeletal muscles of lambs. The experiment was conducted on 48 lambs divided into 3 groups, each consisting of 16 animals: group I (the control group) - received no additional selenium, group II received 0.2 mg Se/day/animal (Se enriched yeast) for 1 month, group III - received 0.2 mg Se/day/animal (Na₂SeO₃) for 1 month. Tissue samples for analyses were collected after slaughter, after 2 and 4 weeks of the oral administration of selenium and in the 6th and 8th weeks of treatment (after the end of selenium supplementation). Selenium was determined in the samples after microwave mineralization using the atomic absorption spectrometer SpektrAA 220Z with electrothermal atomization and Zeeman background correction, Varian. The research results revealed that selenium supplement has increased selenium concentration in lamb tissues and organs. The best performance was observed in the lamb group fed the yeast selenium improved diet. The highest selenium level was determined in lamb kidneys and liver, while the lowest in the heart. The selenium content in lamb tissues and organs recorded in the final stage of the experiment indicated a deficit of this element in the organism. Therefore, it is recommended to improve lamb diet with a selenium dose established on the basis of the selenium status of the animal’s organism as well as to determine selenium content in the sources of this element.
The studies were carried out in two stages. The aim of the first stage was to assess the selenium levels in the feed for lambs, as well as in the milk and serum of their dams. The objective of the second stage of studies was to evaluate the impact of using two different doses and sources of selenium (sodium selenite or Se-enriched yeast) on its concentration in lamb serum. The experiment was conducted on 64 lambs divided into four groups, each consisting of 16 animals: group I, the control group - received no additional selenium, group II - received 0.1 mg Se/day/animal (Se-enriched yeast), group III - received 0.2 mg Se/day/animal (Se-enriched yeast), group IV - received 0.2 mg Se/day/animal (Na₂SeO₃). The concentration of Se in feed, milk and serum was determined by means of an atomic absorption spectrometer SpektrAA 220Z with electrothermal atomization and Zeeman background correction (by Varian). Low concentration of selenium in soil, plants and food would suggest a deficiency of this mineral. These results indicate that low Se levels in the serum of lambs generally reflect the level of dietary Se. The results of this study demonstrate that Se from organic sources was markedly more available to lambs than selenium from mineral sources.
Selenium is an essential element for the proper functioning of mammalian organisms. In many regions of the world, selenium is deficient in the human diet, which is the cause of many diseases. Meat products can be a good source of selenium in the human diet, and pork is traditionally consumed in Poland. Enriching pig feed with selenium can increase its content in pork. The study showed that supplementing the diet of young pigs with various forms of Se in amounts up to 0.5 mg/kg does not decrease fattening parameters or carcass quality. The addition of an organic form of Se had a more favourable effect than inorganic Se on Se concentration in organs and tissues and on the quality of the pork.
Badano wpływ selenu podawanego w postaci drożdży selenowych na procesy oksydacyjne w doświadczalnej hipercholesterolemii indukowanej podawaniem masy jajecznej u królików.