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Seed dormancy is of particular importance in the cultivation of cereals, as it directly affects the quality of crop yield. If the dormancy period is too short, this may lead to pre-harvest sprouting, whereas a dormancy period that is too long may cause uneven germination; both of these scenarios are associated with economic losses. Most enzymes engaged in the metabolism of abscisic acid (ABA) have been identified, and significant progress has been made in understanding the role of this phytohormone in the induction and maintenance of dormancy, mainly as a result of research conducted in Arabi-dopsis. Much less is known about the metabolism and function of ABA in cereal grains, especially in relation to dormancy and germination. This review focuses on the regulation of ABA metabolism in dormant and non-dormant cereal grains, in both the dry state and upon imbibition. Moreover, this review describes the influence of factors such as after-ripening, light, temperature, nitric oxide, and reactive oxygen species (ROS) on the dormancy and germination of cereal grains. These factors, with the exception of ROS, appear to affect the level of dormancy and germination of grains through regulation of ABA metabolism.
From an ethylmethane sulphonate-mutagenized M₂ population of Arabidopsis thaliana L. var Landsberg erecta, a mutant was isolated on the basis of its ability to germinate in the presence of a germination inhibitory concentration (0.35 mM) of spermine. The mutant produced yellowish green seeds that lacked a mucilaginous sheath, exhibited reduced dormancy and were generally viviparous under ambient conditions. Dose-response assays indicated increased resistance of the mutant to spermine but normal sensitivity to spermidine, putrescine and abscisic acid. The spermine resistance and the associated phenotype of the mutant was inherited as a single recessive nuclear mutation. Following the genetic analysis, spermine-resistant mutant has been designated as spr2. The results suggest a role for spermine in seed dormancy.
The investigation of dormancy release in Aesculus hippocastanum seeds was aimed at estimating the proportion of coat-imposed to embryo dormancy, and studying the growth initiation providing embryo dormancy release. During winter, horse chestnut seeds exhibited 16–17 week-lasting deep dormancy, which predominantly was determined by coat-imposed dormancy. Embryo dormancy lasted for 11–12 weeks of wet cold stratification. Embryo dormancy was weak, even the embryo axes excised from deeply dormant seeds were capable of extending to the size exceeding the axis length in intact seeds at radicle protrusion. Embryo dormancy release manifested itself in gradually increasing growth capacity of both embryo axes and cotyledonary petioles. The growth initiation in horse chestnut seeds occurs only by cell elongation. During growth initiation, a more rapid fresh weight gain was observed in comparison with length increment, thus indicating that accumulation of osmotically active substances and active water uptake by embryo axis cells were ahead of their increasing longitudinal cell wall extensibility. Cell wall loosening appeared to be directly related to embryo dormancy release. The hormonal regulation of embryo dormancy release in horse chestnut seeds is discussed.
Changes in hydrotime model parameters were determined in Matricaria maritima L. subsp. inodora seeds during burial in a field in order to describe the seasonal dormancy pattern. Seeds were exhumed at regular intervals over a year and incubated at different water potentials at 19°C. Germination time courses were analyzed to determine hydrotime population parameters. Values of ѱb(50), ѲH and σѱb varied each month. Mean base water potential values in seeds exhumed each month were related to precipitation over 20 days before their exhumation. Soil temperature could be a trend-controlling factor of this relationship. The seeds were in deep dormancy after remaining 80-90 days in soil below or above limit temperature 15°C. The application of the hydrotime model to describe and predict seasonal dormancy patterns of weed seed is promising, especially for species with a considerable diversification of life strategies and ecophysiological flexibility of diaspores. It could also suggest mechanisms of seasonal dormancy changes of seeds in natural conditions and provide a basis for their examination. One of advantages of the dormancy pattern description of weed seeds remaining in a soil bank by means of threshold models is its simplicity.
In several tulip cultivars propagated in vitro, a high decrease in propagation rate was observed after the 4th - 6th multiplication cycles. In vitro shoot cultures of the model tulip cultivars Blue Parrot and Prominence with relatively stable and high multiplication rate and shoots of new Polish cultivar Fringed Black which revealed a decreased regeneration capacity were used in the experiment. Shoots, multiplied at 8-week subculture period on MS modified medium supplemented with thidiazuron (TDZ) and a-naphthaleneacetic acid (NAA), were treated with low temperature (5°C) for 8, 10 or 12 weeks and with 1 mg·dm⁻³ GA₃ which was added to the medium prior to cooling at the 3rd week of multiplication subculture. The significant enhancement of shoot multiplication by cooling was found for the cultivar Fringed Black only. Low temperature treatment for 10 - 12 weeks markedly increased multiplication rate from 2.5 (non-cooled control) to 5.8 - 6.4 (in the 1st subculture). The effect of increased regeneration capacity was maintained during 8 months of cyclic multiplication. Application of GA₃ decreased affect shoot multiplication rate. It is suggested that the decrease in multiplication rate of cv. Fringed Black could result from dormancy development in shoots because the restoration of regeneration capacity occurred after low temperature treatment.
During the development and ripening of triticale caryopses cv. Grado, a gradual increase in the precocious germination ability of the grain was observed. After 48 hrs of incubation (at 22 °C under optimal moisture conditions for germination in darkness) of the freshly-collected caryopses of different ripeness, the percentage of polysomes in the ribosomal fraction isolated from embryonic tissue in buffer A + PTE (to yield released FP + MBP) was about 15 % in the sample of grains gathered at the milk-ripeness stage, over 27 % at the wax-ripeness stage and over 63 % at the full-ripeness stage. Percentages of polysomes solubilized in the pellets in buffer U (TBP, putative cytoskeleton-bound polysomes), were always higher and amounted to about 36 % at the milk-ripeness stage, 58 % at the wax-stage and 68 % at the full-ripeness stage. The incorporation of 3H-uridine into polysomal RNA (mRNA+rRNA) of triticale embryos increased during the development and maturation of the caryopses subjected to germination from about 19 and 33 Bq/mg RNA at the milk-ripeness in the FP+MBP and the TBP to 220 and 312 Bq·mg⁻¹ RNA at the full-ripeness stage, respectively. The effect of the pericarp and other tissues surrounding the embryo was also investigated. Removal of the outer pericarp strongly stimulated germination of unripe caryopses in the middle period of development e.g. 32 and 39 DAF. The strongest stimulatory effect on transcription was found in the embryo during germination, in all samples of caryopses of various degrees of ripeness, by isolating it completely from the pericarp, testa and endosperm. The high percentage of polysomes in the TBP and the high incorporation 3H-uridine into RNA in that fraction during precocious germination suggests an important role for this sub-population of polysome (and the proteins synthesised by them) in initiation of precocious germination processes.
Experiments were done to determine the effects of cold stratification (0, 20, 40, 60, 80 or 100 days), application of gibberelic acid (GA3) (0, 250, 500, 1000 or 2000 mg/l) and the combination (GA3 + stratification) on seed germination of black mulberry. Application of 1000 mg/l GA3 proved more effective than any of the other concentrations of GA3 applied. Seeds stratified for 100 days showed 88% germination. The combined treatment of 250 mg/l GA3 and 100 days of stratification yielded 96% germination of seeds. The relationships between GA3 concentration and seed germination (r = 0.93), and between stratification duration and seed germination (r = 0.91) of black mulberry were linear.
An acidic condition (low pH) of the germination media promoted dormancy breakage of scarified seeds of Townsville stylo (Stylosanthes humilis H.B.K.), an annual tropical forage legume, whether produced by either an unbuffered (HCl-KOH) or a buffered (phthalate, Mcllvaine) medium. Except for aminooxyacetic acid, all ethylene biosynthesis inhibitors tested and supplied with the low pH solutions decreased germination to variable extents. Low pH-stimulated dormant seeds produced ethylene 4-fold as much than untreated seeds. Production of ethylene by seeds treated with high pH solutions, which did not affect their dormant state, was also very low.
This study was conducted on barley cv. Ars. caryopses collected at full ripeness and divided into two batches. From one batch (dormant caryopses) polysomes were isolated from embryos immediately after harvesting and after two days of germination. From the other batch (non-dormant caryopses) the same was done after eight months storage in a dry state. A low ionic strength cytoskeleton-stabilizing buffer was used for the isolation of polysomes. Four different fractions of polysomes were examined: free polysomes (FP), membrane-bound polysomes (MBP), cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP). In germs grown from non-dormant caryopses, the first two fractions (FP + MBP) made up about 78 % of the total ribosomal material, whereas in embryos of dormant, imbibed caryopses, two last fractions (CBP + CMBP) made up about 71 %. The percentage of polysomes after 48 hours of imbibition of dormant caryopses in the FP, MBP and CBP was only about 13 % (i.e., 87 % monosomes), whereas a greater proportion (19.4 %) was found in the CMBP. The highest incorporation of ³H-uridine and ¹⁴C-amino acids (after 48 hours of germination and 0.5, 3 and 6 hrs incubation with precursors) took place in trhc CMBP both in dormant and non-dormant caryopses The major amount of the two polysome fractions associated with the cytoskeleton (CBP and CMBP) and the higher activity of CMBP in protein synthesis in embryos of dormant, imbibed triticale caryopses may indicate a significant role for polysomes associated with the cytoskeleton in the control of protein synthesis in dormant and germinating caryopses.
Juniperus oxycedrus subsp. macrocarpa is an endangered species in southwest Spain, with seed dormancy as found in other species of the same genus. This study employed different experiments to determine a method to improve the seedling emergence in this species. Three types of seedling emergence trials were performed: (a) untreated seeds under greenhouse conditions, (b) untreated seeds under natural conditions, and (c) treated seeds under greenhouse conditions, with different acids (sulphuric, hydrochloric and nitric) for 10 and 30 min, followed or not by cold stratification for 3 months. In all trials, seeds derived from both mature and immature cones were used to verify which one produced higher seedling emergence. Previously, seed viability was verified and a proper substrate for greenhouse sowing was selected. The best percentage of seedling emergence was obtained in the "a” and "b" trials. In trial "a", seeds derived from immature cones germinated significantly better than mature ones. Chemical scarification of seeds with or without cold stratification yielded less seedling emergence than the other trials.
The camaroid graptolite Xenotheka klinostoma Eisenack, 1937 is described from the lower Llanvirn limestones of Gilbergabrottet, northern Öland, Sweden. Two distinct autothecal morphs are recognized: (1) normal morph (described for the first time), i.e. an autotheca with an unsculptured outer surface, devoid of both an outer lining and autothecal occlusion, and inhabited by an active zooid; and (2) sealed morph, i.e. an autotheca coated and occluded, provided with a sculptured outer lining made of a unique verrucose fabric, and inhabited by an inactive or dormant zooid. In addition, the existence of a hypothetical (3) unsealed morph or re−opened autotheca, devoid of an autothecal occlusion but provided with an outer lining, and inhabited by a reactivated zooid, is predicted. The sealed morphs may represent an adaptation which allowed their inhabitants to survive adverse conditions. The outer lining of Xenotheka is compared with a peculiar outer membrane found in the modern hemichordate Rhabdopleura, from the intertidal zone of Fiji, and with camaroid extracamaral tissue.
The ecophysiological regulation of seed dormancy in perennial species and those with a varied life cycle has not been studied in detail yet. That is why an attempt has been made to determine the Cirsium arvense seed water relations during stratification and afterripening at different temperatures and germination at constant or fluctuating temperatures on the basis of the hydrotime model. The obtained results showed that breaking of the primary dormancy of achenes took place only during the first stratification month at moderate temperatures, mainly due to an increase in the average water-stress tolerance in a seed population. The induction of secondary seed dormancy during after-ripening at all temperatures resulted mostly from a substantial loss of the seeds' ability to tolerate water stress. Fluctuating temperatures affected neither seed germination nor the hydrotime model parameters. The analysis of the variations of hydrotime model parameters allows a better understanding of the physiological basis of seed dormancy relief and induction.
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