Wyniki wyszukiwania

1

Solubilization and one-step purification of mannosylphosphodolichol synthase from Trichoderma reesei

100%

Kruszewska J. S., Perlinska-Lenart U., Palamarczyk G.

Acta Biochimica Polonica

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1996

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tom 43

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nr 2

Mannosylphosphodolichol synthase (MPD-synthase) (EC 2.4.1.830) catalyzing formation of MPD from GDPMan and dolichylphosphate (PD) has been purified from T. reesei cellular membranes almost to homogeneity. Selective solubilization of the enzyme was followed by one step purification on Phenyl-Sepharose column. SDS/ PAGE of the purified enzyme fraction revealed the presence of a protein band of 31 kDa corresponding to the apparent molecular mass of the MPD-synthase purified from S. cerevisiae [Babczinski, P. et al. (1980) Eur. J. Biochem. 105,509-515; Haselbeck A. (1989) Eur. /. Biochem. 181, 663-6681. During solubilization, the enzyme was stabilized by the presence of a lipophilic substrate dolichylphosphate and phospholipids as well as by protease inhibitors. The Phenyl-Sepharose purified enzyme had an absolute requirement for dolichylphosphate and was activated by cAMP dependent protein kinase.

2

Chemical synthesis of dolichyl phosphates, their analogues and derivatives and application of these compounds in biochemical assays

86%

Danilov L. L., Druzhinina T. N.

Acta Biochimica Polonica

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2007

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tom 54

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nr 4

695-701

Several methods for simple and efficient chemical synthesis of dolichyl phosphates and their an-alogues and derivatives are briefly summarized with a special emphasis on chemical modifica-tion of phosphoryl group and preparation of dolichyl phosphates labelled at the ω-end and at the γ-isoprene unit of the isoprene chain by fluorescent groups, 2-aminopyridine and 1-aminon-aphtalene residues. Additionally, data on biochemical assays with application of the compounds mentioned above are presented.

3

Fungal glycoproteins and their biosynthetic pathway as potential targets for antifungal agents

72%

Tanner W., Gentzsch M., Immervoll T., Scheinost A., Strahl-Bolsinger S.

Acta Biochimica Polonica

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1995

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tom 42

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nr 4

The yeast cell wall as a good antifungal target is discussed in general. More specifically the reaction, catalyzed by Dol-P-Man: protein O-D-mannosyltransferase is proposed as a new potential target. Six genes responsible for this endoplasmic reticulum-localized reaction have been cloned and characterized so far. Triple disruptions of these genes are either lethal or the corresponding cells have to be osmotically stabilized to survive. No inhibitors of this reaction are as yet known.

4

Dolichyl sulphate and H-phosphonate: Enzymatic reactions with activated sugars

58%

Sizova O. V., Maltsev S. D., Shibaev V. N., Jankowski W. J., Chojnacki T.

Acta Biochimica Polonica

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1998

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tom 45

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nr 4

Two phosphate-modified analogues of dolichyl phosphate were evaluated as substrates or inhibitors of the reactions catalyzed by mammalian microsomal enzymes. Dolichyl H-phosphonate could serve as an efficient acceptor for mannosyl and glucosyl transfer. The reaction products were chromatographically different from those formed from dolichyl phosphate. Lower activity of the H-phosphonate was observed for the reaction of N-acetylglucosaminyl phosphate transfer from UDP-GlcNAc. Dolichyl sulphate was shown not to serve as a substrate for the transfer of mannosyl (from GDP-Man), glucosyl (from UDP-Glc) or N-acetylglucosaminyl phosphate (from UDP-GlcNAc) residues in the presence of rat liver microsomes. Weak inhibitory properties of this analogue were demonstrated.

Ograniczanie wyników

4 Acta Biochimica Polonica

1 Chojnacki T.

1 Danilov L. L.

1 Druzhinina T. N.

1 Gentzsch M.

1 Immervoll T.

1 Jankowski W. J.

1 Kruszewska J. S.

1 Maltsev S. D.

1 Palamarczyk G.

1 Perlinska-Lenart U.

1 Scheinost A.

1 Shibaev V. N.

1 Sizova O. V.

1 Strahl-Bolsinger S.

1 Tanner W.

1 2007

1 1998

1 1996

1 1995

Solubilization and one-step purification of mannosylphosphodolichol synthase from Trichoderma reesei

Chemical synthesis of dolichyl phosphates, their analogues and derivatives and application of these compounds in biochemical assays

Fungal glycoproteins and their biosynthetic pathway as potential targets for antifungal agents

Dolichyl sulphate and H-phosphonate: Enzymatic reactions with activated sugars