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1

Purification and partial characterization of glutamine synthetase isoforms from Triticale seedlings

100%
Bielawski W.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 4
Two isoforms of glutamine synthetase (EC 6.3.1.2), cytoplasmic (GSi) and chloroplastic (GSi) were isolated from shoots of 14-day-old Triticale seedlings, and purified 260-fold and 248-fold, respectively. Specific activities of the two preparations were 35.1 and 33.5 nmol x min-1 per mg of protein, respectively. Both crude extracts and homogeneous GSi and GS2 preparations required divalent metal ions (Mg2+, Mn2+, Co2+) for their activities. Mg2+ was the most effective activator, the highest activity of GSi being reached at 5 mM, and that of GS2 at 20 mM MgCl2- The optimum pH for the two isoforms showed large differences, dependent also on the kind of divalent ion. Molecular masses of GSi and GS2 were 305000 Da and 385200 Da, respectively. It seems that native protein of GSi is built from eight identical subunits of Mm 38000 Da and that of GS2 of the same number of subunits but of Mm of about 48000 Da. Proteins of GS isoforms differed significantly in their amino-acid composition.
2

Modulation of 5-N-alkylresorcinol hemolytic properties by divalent cations. Dependence of the effect of cations on alkylresorcinol structure

86%
Stasiuk M., Kozubek A.
Cellular and Molecular Biology Letters
|
1997
|
tom 02
|
nr 1
It was demonstrated that the process of hemolysis of erythrocytes induced by resorcinolic lipids is inhibited by divalent cations present in the incubation medium. The extent of inhibition is dependent on the nature of both type of the cation and the hemolytic agent. Lysis most susceptible to divalent cations is that induced by short (C15) and medium-chained (C19) 5-n-alkylresorcinols, which also have the highest observed hemolytic potencies. Furthermore, the protection was the stronger the stronger lytic activity was exerted by studied compound. The homologs with the longest side chain studied, although exhibiting the lowest hemolytic activity by themself, were the least susceptible to inhibition of their hemolytic activity by cations. The effect of cobalt cation has been found to be intermediate between the effect of Mn2+ and Zn2+. The most effective in protection of erythrocytes was Zn2+ which almost completely protected the cells against alkylresorcinol-induced lysis at the concentrations of 10-6 M and above.
3

Divalent cations and protons inhibit Triton X-100 induced ion channels

86%
Rostovtseva T. K.
Biophysics of Membrane Transport
|
1994
|
tom 12
|
nr 2
4

Modulation of hemolytic properties of resorcinolic lipids by divalent cations

86%
Stasiuk M., Kozubek A.
Cellular and Molecular Biology Letters
|
1996
|
tom 01
|
nr 2
The effect of divalent cations in concentrations 10-9-10-3M upon the hemolysis of sheep erythrocytes induced by resorcinolic lipids was studied. It was demonstrated that the process of hemolysis is inhibited by divalent cations present in the incubation medium. The extent of inhibition is dependent on both type of the cation and hemolytic agent. In alkylresorcinol-induced hemolysis the most effective in protection of the cells were Zn2+ and Co2+ cations. Weaker protective action was observed for Cd2+ and Mn2+. Calcium ions, in all studied concentrations, were without marked effect on the level of relative hemolysis. Magnesium ions as well as Ba2+ in nanomolar concentrations were effective inhibitors of the lysis but this effect diminished at higher (millimolar) concentrations of these ions. When alkenylresorcinols were used as hemolysis-inducers no effective protection of erythrocytes by Ca2+, Ba2+ or Mg2+ was observed. Most effective in protection of cells against this amphiphile-induced lysis, similarly to their effect observed previously for various lytic agents, were cations of Zn2+, Co2+ and Cd2+. Mn2+ cations were intermediate, with respect to their protective potency.
5
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Interrelations between DNA concentration and physicochemical parameters in the loess soil profile

58%
Wolinska A., Stepniewska Z., Woloszyn A., Rzewuska M.
Acta Agrophysica
|
2012
|
tom 19
|
nr 2
The purpose of presented study was determination of the impact of selected physicochemical parameters, strongly influencing the soil biological life: water potential (pF), oxygen availability for microorganisms (ODR), redox potential (Eh), content of Mg, Ca and total organic carbon (TOC) on soil DNA concentration. Undisturbed loess soil material was taken to metal cylin-ders (100 cm3) from four depths (0-20; 20-40; 40-60 and 60-80 cm), what make possibility for description of DNA content and its distribution in the whole soil profile. Our results revealed signif-icant (p<0.05) positive relationships between soil DNA content and measured values of ODR (r = 0.94***), Eh (r = 0.52**) and TOC (r = 0.98***), what were confirmed by high values of correla-tion coefficients (r). Whereas, significant negative interrelationships between soil DNA and pF (r = –0.57*) or Mg content (r = –0.79***) were determined. However, in the current experiment condi-tions, we did not found significant correlation between Ca presence and DNA content (p>0.05). Significant (p<0.05) decrease of DNA concentration by 62.8% with an increase of soil depth was noted, what was undoubtedly connected with spatial distribution of microorganisms in the soil profile and its likes for surface layers colonization.
6

Comparison of short-term cadmium poisoning in the shrimp Crangon crangon from the Baltic Sea and the shrimp Palaemon serratus from the Atlantic Ocean with cadmium bioaccumulation and malic enzyme activity in abdomen muscle

51%
Napierska D., Thebault M. T., Pempkowiak J., Skorkowski E.
Oceanologia
|
1997
|
tom 39
|
nr 2
7

Hemolytic activity of the non-ionic detergents Tween 80 and Triton X-100

44%
Bielawski J., Mrowczynska L., Konarczak M.
Biological Bulletin of Poznań
|
1995
|
tom 32
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