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The present study investigated the effectiveness of three different disinfectants: preparation H1 (two-component preparation based on hydrogen peroxide); Pedox (multi-component preparation based on peroxyacetic acid) and Savo hypochlorite preparation) against Malassezia pachydermatis. The antifungal activity of disinfectants was tested by quantitative suspension method according to STN EN 1650. The results confirmed 100% effectiveness of these disinfectants at all concentrations and exposure times tested.
Background. Evaluation of the biocidal activity of chemical disinfectants and antiseptics according to European Standards (EN) is based on determination of the reduction of the number of viable test microorganisms under defined conditions. Objective. The objective of this study was to investigate whether reducing the neutralization time required following declared product contact times for the tested microorganisms yields method validations. Material and methods. This study was conducted on 14 products containing active substances from different chemical groups: alcohols, aldehydes, biguanides, quaternary ammonium compounds, phenols, amines derivatives, oxidizing agents. These products were tested according to phase 1 tests: EN 1040:2005 and EN 1275:2005 and then according to phase 2, step 1 tests: Draft EN 13727:2005 and EN 13624:2003. Biocidal activity was evaluated using the following test organisms: S. aureus ATCC 6538, P. aeruginosa ATCC 15442, E. coli NCTC 10538, E. coli ATCC 10536, E. hirae ATCC 10541, C. albicans ATCC 10231 and A. brasiliensis ATCC 16404. Results. Validation C results for all products and tested microorganism strains were at least half of the density of the suspension for validation (Nvo) after only 10 s of neutralization. Furthermore, results from test procedures performed in parallel were also positive except 5 products toward A. brasiliensis. Conclusions. The results of our study confirm that the contact time described in the European Standards phase 1: EN 1040 and EN 1275, as well as phase 2, step 1: Draft EN 13727 and EN 13624 can be precisely determined in spite of reducing the neutralization time from 5 minutes to even 10 seconds.
Quaternary ammonium compounds are broad-spectrum bacteriocides widely used as antiseptics, disinfection and preservation agents. The aim of this study was to examine the activity of two quaternary ammonium salts, cetylpyridinum bromide and a newly synthesized quaternary bis ammonium salt, against S. epidermidis biofilm. The average values of killing efficiency for cetylpyridinum bromide ranged from 26.6% to 64.1% for all tested concentrations (0.125 to 8.0 μg×mL⁻¹) and for quaternary bis ammonium salt the percentage of killing efficiency ranged from 59.7% to 88.4% for tested concentrations (from 2.0 to 128.0 μg×mL⁻¹). Both tested compounds significantly affect staphylococcal biofilms, but any of used concentrations caused a total eradication of bacterial biofilm.
The natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. The aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. A universally applied disinfecting agent was used in the tests. Bacterial strains: Listeria innocua, Pseuciomonas putida. Micrococcus luteus, Staphylococcus hominis strains, were isolated from food contact surfaces, after a cleaning and disinfection process. The disinfecting agent was a commercially available acid specimen based on hydrogen peroxide and peroxyacetic acid, the substance that was designed for food industry usage. Model tests were carried out on biofilm formed on stainless steel (type 304, no 4 finish). Biofilms were recorded by electron scanning microscope. The disinfecting agent in usable concentration, 0.5% and during 10 minutes was ineffective for biofilms. The reduction of cells in biofilms was only 1-2 logarithmic cycles. The use of the agent in higher concentration - 1% for 30 minutes caused reduction of cell number by around 5 logarithmic cycles only in the case of one microorganism, M. luteus. For other types: L. innocua, P. putida, S. hominis, the requirements placed on disinfecting agents were not fulfilled. The results of experiments proved that bacterial biofilms are resistant to the disinfectant applied in its operational parameters. Disinfecting effectiveness was achieved after twofold increase of the agent's concentration.
Background. The ability of bacteria to develop common mechanisms of resistance to antibiotics and disinfectants raises doubts about the effectiveness of disinfection processes. Glucoprotamin (GP) is an antimicrobial active substance which is widely used to the disinfection in medical area. Objective. The aim of study was to compare GP’s effectiveness with susceptibility of reference strains used for the evaluation of bactericidal efficacy of disinfectants Staphylococcus aureus (S. aureus); Pseudomonas aeruginosa (P. aeruginosa) and standard antibiotic-resistant strains: meticillin-resistant S. aureus (MRSA) and tetracycline-resistant P. aeruginosa (PAO-LAC). Materials and Methods. Minimum inhibitory concentrations (MICs) of GP and minimum bactericidal concentrations (MBCs) against tested strains were evaluated by serial broth dilution technique. GP’s efficiency was examined according to qualitative (phenol coefficient GP-PC) and quantitative (EN 1040: 2006) test methods. Results. Gram-negative strains were more tolerant to GP than Gram-positive strains among tested strains. MRSA and S. aureus exhibited similar susceptibility to GP. PAO-LAC had significantly lower susceptibility to GP than P. aeruginosa (P≤0,05). There were no differences in GP efficiency against these strains based on GP-PC. According to PN-EN 1040: 2006 standard average obligatory reduction ≥ 5 log10, was demonstrated in the active concentration of GP (84 mg/l) at obligatory 5 min contact time for PAO-LAC and P. aeruginosa. The differences in basis bactericidal activity between PAO-LAC and P. aeruginosa were obtained in the active concentration at 10 and 15 min contact time (P≤0,05). Conclusions. Variation in a susceptibility of reference strains and antibiotic-resistant standard strains has no meaning at used clinically GP concentrations, which are higher than concentration causing basis bactericidal activity of GP.
Chlorine dioxide is widely used as a pre-oxidant and disinfectant but it has the disadvantage of inducing the formation of the inorganic by-products chlorites and chlorates. It is therefore of interest to decrease the chlorine dioxide demand and oxidation, and/or removal of natural organic matter (NOM). The objective of this study was to estimate the efficiency of the adsorption of oxidised and unoxidised NOM on granular activated carbon (GAC) filters. For this purpose, three pairs of columns filled with three types of GAC were set. The study showed that the efficiency of adsorption increased after the pre-oxidation of NOM with ClO2. GAC filters also efficiently removed the unwanted inorganic by-products.
The applied disinfectants affected the improvement of physicochemical parameters of litter by decreasing its moisture and pH. It was demonstrated that the addition of decontaminating agents to litter resulted in a beneficial decline in the microflora of poultry house air. The total count of mesophilic bacterium was reduced most effectively by means of a disinfectant, whereas the total number of fungi and moulds – by calcium oxide (CaO). But no differences were observed in ammonia concentration in the air of the hen houses examined. In poultry houses in which disinfectants were added to litter, the reported death rate and culling percentage were lower as compared to a control broiler house. Birds reared in a broiler house in which litter was disinfected with CaO achieved the highest European Index of Productivity (EIP).
Porównano wrażliwość na środki dezynfekcyjne szczepów Klebsiella pneumoniae: wyizolowanego ze środowiska szpitalnego (Ks) i pochodzącego z muzeum szczepów PZH (K28) z wrażliwością testowego szczepu E. coli NCTC 8196. Zbadano działanie 7 preparatów dezynfekcyjnych. Szczepy Klebsiella wykazały większą wrażliwość na środki dezynfekcyjne niż E. coli. Jedynie w przypadku formaliny zależność ta była odwrotna.
Stwierdzono, że podwyższenie temperatury o 10°C (w zakresie temp. 22-32°C) zwiększa aktywność bakteriobójczą środków dezynfekcyjnych takich jak: Laurosept, Chloramina, Sterinol i Jodoseptan w stosunku do E. coli i S. aureus. Nie wpływa natomiast na działanie bakteriobójcze Septylu na S. aureus. Preparaty powierzchniowo czynne działają bardziej skutecznie w podwyższonej temperaturze na S. aureus, a preparaty fenolowe i Chloramina na E. coli.
Porównano wrażliwość na 6 środków dezynfekcyjnych 4 standardowych szczepów bakterii pochodzących z hodowli 24 h, 48 h, 72 h. W większości przypadków najmniejszą wrażliwością charakteryzowały się szczepy hodowane 24 h.
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