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1

Antimicrobial activity of 2,4-dihydro-[1,2,4]triazol-3-one derivatives

100%
Stefanska J., Struga M., Tyski S., Kossakowski J., Dobosz M.
Polish Journal of Microbiology
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2008
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tom 57
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nr 2
Antibacterial and antifungal activity of 2,4-dihydro-[1,2,4]triazol-3-one derivatives were examined by the disc-diffusion method (growth inhibition zone diameter in agar medium). The MIC's for the most active agents were determined. Of all the tested compounds, aminomethy1 derivatives of 2,4-dihydro-[1,2,4]triazol-3-one exhibit activity against the majority of microorganisms studied.
2

Investigation of antibacterial properties of Cotinus coggygria from Turkey

86%
Tunc K., Hos A., Gunes B.
Polish Journal of Environmental Studies
|
2013
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tom 22
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nr 5
Today it is quite important to develop new, reliable, cost-efficient, and non-toxic herbal anti-microbial agents in order to minimize environmental and health problems. This study uses the disc diffusion method to examine the antimicrobial activity of the extracts of Cotinus coggygria Scop. prepared in ethanol, methanol, distilled water, chloroform, acetone, and petroleum ether against the bacteria Staphylococcus epidermidis, Escherichia coli, Salmonella typhimurium, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis. Cotinus coggygria was found to inhibit the reproduction of microorganisms at various rates. The plant extracts in distilled water and methanol were found to be the most effective against Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis.
3
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Susceptibility testing of Aspergillus niger strains isolated from poultry to antifungal drugs - a comparative study of the disk diffusion, broth microdilution (M 38-A) and Etest methods

58%
Tokarzewski S., Ziolkowska G., Nowakiewicz A.
Polish Journal of Veterinary Sciences
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2012
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tom 15
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nr 1
The aim of this study was to determine the sensitivity of Aspergillus niger strains isolated from birds to available antifungal drugs using different in vitro assays - classical disk diffusion, Etest® and broth microdilution NCCLS/CLSI M 38-A. The study material consisted of about 2.000 swabs and samples from different species of birds. A. niger (n=10) was accounted for 6.81% of the total pool of strains isolated. Determinations were made for 13 antifungal drugs using the disk diffusion method. The A. niger exhibited high susceptibility to enilconazole, terbinafine, voriconazole, tioconazole and ketoconazole, low susceptibility to clotrimazole, miconazole and nystatin, and resistance to amphotericin B, itraconazole, pimaricin, fluconazole and 5-fluorocytosine. Minimum inhibitory concentration (MIC) was determined for 9 antifungal drugs using the micromethod of duplicate serial dilutions in a liquid medium. A. niger strains were most susceptible to enilconazole and voriconazole. MIC ranged from 0.0625 to 0.5 μg/ml for enilconazole, with MIC90-0.5 μg/ml and MIC50-0.125 μg/ml. The corresponding values for voriconazole were 0.25-1 μg/ml, 1 μg/ml and 0.5 μg/ml. MIC for amphotericin B and terbinafine ranged from 0.5 to 4 μg/ml, while the values for the remaining drugs were highly varied. MIC was measured by the gradient diffusion method using Etest® for 5 antifungal drugs: amphotericin B, fluconazole, itraconazole, ketoconazole and voriconazole. By far the highest susceptibility was obtained in the case of voriconazole, with MIC ranging from 0.0625 to 1 μg/ml. MIC for amphotericin B ranged from 0.25 to 4 μg/ml, for itraconazole and ketoconazole ranging from 0.5 to 16 μg/ml. Methods available for this purpose are not always applicable in field conditions. The present results indicate that the Etest® technique, due to its high percentage of agreement with the M 38-A microdilution method, should find application in medical and veterinary practice.
4
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Antimicrobial properties of 6-bromoeugenol and eugenol

58%
Mahboub R., Memmou F.
International Letters of Natural Sciences
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2016
|
tom 53
We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains: Pseudomonas aeruginosa (S1), Escherichia coli (S2) and Staphylococcus aureus (S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with the Pseudomonas aeruginosa (S1) at all concentrations and with the Escherichia coli (S2) at 0.5 mg/ml. We remark that the Pseudomonas aeruginosa (S1) is the more sensitive strain than Escherichia coli (S2) and Staphylococcus aureus (S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.
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