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The paper reports chromosome numbers for 13 taxa of Elatine L., including all 11 species occurring in Europe, namely E. alsinastrum, E. ambigua, E. brachysperma, E. brochonii, E. californica, E. campylosperma, E. gussonei, E. hexandra, E. hungarica, E. hydropiper, E. macropoda, E. orthosperma, E. triandra originating from 17, field-collected populations. For seven of them (E. ambigua, E. californica, E. campylosperma, E. brachysperma, E. brochonii, E. hungarica, E. orthosperma) the chromosome numbers are reported for the first time. With these records, chromosome numbers for the whole section Elatinella Seub. became available. Although 2n = 36 was reported to be the most common and the lowest chromosome number in the genus, our data show that out of thirteen species analyzed, six had 36 chromosomes but five species had 54 chromosomes, and the lowest number of chromosomes was 18. These data further corroborates that the basic chromosome number in Elatine is x = 9.
Endoreduplication of nuclei is a common phenomenon in plants. Cells with multiplied DNA content are usually termed endopolyploid or polysomatic. The study was aimed at clarifying the chromosomal status of endoreduplicated nuclei. So far it has been generally assumed that endoreduplicated nuclei represent a form of polyploidy. We propose a distinction between true polyploid nuclei, possessing multiple sets of chromosomes, and chromosomes formed within endoreduplicated nuclei. We supposed that chromosomes within endoreduplicated nuclei are not separated, as in true polyploids, but rather are bound together. To clarify these two alternatives, we subjected interphase nuclei exhibiting different degrees of endoreduplication and ploidy to fluorescent in situ hybridization, using diploid and tetraploid cabbage root tips containing nuclei of four different sizes (2C-16C or 4C-32C, respectively) as revealed by flow cytometry. Nuclei were hybridized with an rDNA probe (pTA71), which showed four rDNA hybridization sites on diploid metaphase chromosomes. The number of hybridization sites was constant for both diploid and tetraploid samples (3-6 per diploid and 7-12 per tetraploid nuclei) irrespective of endoreduplicated nuclear size. The results showed more signals only in the tetraploid compared to the diploid genotype, but not between different nuclear sizes within a genotype of the same ploidy. We suggest that these results indicate that chromosomes within endoreduplicated nuclei are actually bundles of sister chromatids. The consequences of this understanding of the bundled chromosomal status of endoreduplicated nuclei are discussed.
In four diploid and four tetraploid families correlations were evaluated between resistance to Phytophthora infestans of leaflets, tuber slices and whole tubers as well as correlations between three aspects of resistance and maturity or tuber shape regularity. A virulent P. infestans isolate was used for inoculations. Only in the family D4 a significant positive correlation was found between all the three aspects of resistance. Genotypes with susceptible leaflets and resistant tuber slices were not identified in any family. In some families genotypes with resistant whole tubers and susceptible tuber slices were found; the family D5 was found to have genotypes in which resistance of leaflets was associated with susceptibility of tuber slices. The resistance to P. infestans of leaflets or tuber slices tended to be negatively correlated with both early maturity and regularity of tuber shape.
Retroviral envelope (env)-like sequences in 2 cultivated allotetraploid cottons and their diploid progenitors have been identified and characterized in this study. DNA sequence analysis reveals that these sequences are heterogeneous. The observed sequence diversity, however, seems to preserve coding information. This is evidenced by the detection of the transmembrane domain (TM), which is the most conserved feature of the divergent retroviral env genes. The high ratio of synonymous to nonsynonymous changes suggests that these sequences are evolving under purifying selection. Phylogenetic analysis shows that Gossypium sequences closely cluster with a lineage of plant endogenous retroviruses that have an env-like gene. These results provide evidence for the antiquity and the wide diversity of env-like sequences in the Gossypium genome.
Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The Variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.
Pollen and seed morphology were examined in diploids (2n = 14) and natural tetraploids (2n = 28) of Trifolium pratense L. The pollen morphology was generally correlated with ploidy level. Great variety in pollen size and apertures was noted in the tetraploid form. Exine structure was studied by TEM. Seed size, color and coat surface ornamentation can be distinguished between the diploid and tetraploid forms.
The structural and developmental features of the diploid and tetraploid plants of Arabidopsis thaliana ecotype Wilna were studied by light and scanning electron microscopy (SEM). The diploid and tetraploid plants differ in the following morphological characters: general habit, length of life cycle, and shape and size of leaves, flowers, pollen grains, seeds and fruits. The developmental pattern of generative structures, such as ovule initiation, integument differentiation and seed formation, is similar in the two forms but differs in details. These differences concern the gradual degradation and thickening of integument layers and the deposition of polysaccharides within testa layers. SEM analysis of generative (pollen grains, seeds, fruits) and vegetative organs (stems) shows variation in their surface structure. The siliques and stem surfaces additionally exhibit different tertiary sculpture. Analysis of seeds shows variation in the shape and size of the testa epidermal cells and the curvature of the outer periclinal cell walls. There are significant differences between mature pollen grains in grain size and the number of exine furrows. In the examined plants, micromorphological features of both pollen grains and seeds can be conveniently used as good diagnostic characters for determination of the ploidy level of Arabidopsis thaliana plants.
Variations in seed coat patterns are successfully employed in the establishment of evolutionary relationships. This research addressed the evolutionary implications of the anatomy of the developing seed coat in amphidiploid Brassica species. Light microscopy was used to study the development of seed coat structure in six species (15 accessions): three amphidiploids and their three diploid parents. Four types of epidermis layer, six types of subepidermis and nine types of palisade layer could be recognized during the course of the seed coat developmental process. The types of epidermis and subepidermis layers in diploids and amphidiploids changed similarly during seed development. Although there was little difference in the types of palisade layer among the accessions of diploids and amphidiploids at the early stages, many particular types appeared in these species at middle and later developmental stages. Palisade layer development varied in complicated ways in amphidiploids. Some accessions showed palisade layer types intermediate between the two putative parents, while others resembled only one of the two diploid ancestors. The developmental types of epidermis and subepidermis did not show the relationships between amphidiploids and diploids. However, the development of types of palisade layer apparently can serve as an excellent character indicating the seed coat evolution of amphidiploids.
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