Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: digoxigenin

Sortuj według:

Ogranicz wyniki do:

Different beta-adrenergic-specific molecular probes reveal the same DNA species in Paramecium hybridization analysis

100%

Surmacz L., Krawczyk K., Wyroba E.

Cellular and Molecular Biology Letters

1997

tom 02

nr 4

In Southern blot analysis of Paramecium genomic DNA using β-adrenergic specific oligonucleotides labelled with Digoxigenin-ddUTP, the DNA species of the same molecular size were detected by different molecular probes. The 6.5 kb DNA species hybridized with the probes directed to the adjacent β2-receptor transmembrane domains: to TM3 - including Asp 113 involved in β-agonists and antagonists binding, and to TM4 - the oligonucleotide being one of the "gene-specific universal mammalian sequence-tagged site”. Since a profound physiological effect of beta-agonists and antagonists has been previously observed in Paramecium, such a hybridization pattern may suggest an existence of Paramecium DNA sequences homologous to those of metazoan eukaryotes encoding for a membrane beta-adrenergic receptor.

Comparative studies on sensitivity of digoxigenin - labelled probes and detection systems confirming replication of CDV in Vero cells

58%

Rzezutka A., Mizak B.

Bulletin of the Veterinary Institute in Puławy

2003

tom 47

nr 1

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Cellular and Molecular Biology Letters

1 Krawczyk K.

1 Mizak B.

1 Rzezutka A.

1 Surmacz L.

1 Wyroba E.

1 2003

1 1997

Wyniki wyszukiwania

Different beta-adrenergic-specific molecular probes reveal the same DNA species in Paramecium hybridization analysis

Comparative studies on sensitivity of digoxigenin - labelled probes and detection systems confirming replication of CDV in Vero cells