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Effects of the phosphorus content in a nutrient solution (15 or 50 mg P dm-3), growing substrate (rockwool or coconut fiber) and the plant growth stage (for roots: 71 or 113 days after transplanting DAT; for leaves: 71 or 92 DAT) on the chemical composition of roots, the phosphorus content in leaves and the expression of genes encoding proteins involved in the transport of phosphorus from the medium to the plant were investigated in tomato cv. Admiro F1 grown in a foil tunnel. A fertigation system without recirculation was used. Regardless of the plant age and growing substrate, tomatoes fertilized with a nutrient solution containing 50 mg P dm-3 had more phosphorus, iron, boron and copper in roots and more phosphorus in leaves. Irrespective of the stage of plant growth and phosphorus level in the medium, the content of almost all macro- and microelements was higher in roots of plants grown in rockwool than in coconut fiber. The stage of plant growth significantly affected the mineral composition of roots as well as the P content in tomato leaves. More phosphorus was stored in roots of younger plants, whereas the phosphorus content was lower in younger than in older leaves. Our analysis of the gene expression showed that transporters encoded by LePT1-LePT4 were involved in phosphate nutrition. Expression of the genes was generally (except LePT4) higher in plants treated by the solution containing 15 mg P dm-3 than in plants treated by 50 mg P dm-3. The expression of genes LePT2, LePT3 in roots of older plants (113 DAT) was generally higher than in young plants.
The hydroponic system is very often used for greenhouse vegetable production. The flood irrigation on a bench or on the flood floors is applied in the production of ornamental plants and vegetable transplants. This system is environmentally friendly, since fertigation is used in a closed system using the recirculation of nutrient solution. The aim of this study, conducted in 2010-2012, was to determine the effect of flood fertigation with different concentrations of nutrient solution (EC: 1.0; 2.0; 3.0; 4.0 mS cm-1) on the yield and quality of the butterhead lettuce cultivar Natalia grown in different substrates (coconut coir, peat and rockwool). The experiment was conducted in the spring and autumn cycle. The highest marketable yield at each concentration of the nutrient solution was achieved in the cultivation in rockwool, both within the spring and autumn. The highest nitrate content in lettuce in terms of both cultivation cycles was found in the lettuce grown in the nutrient solution concentration of EC 4 mS cm-1, and the lowest one was achieved in the nutrient solution concentration of EC 1.0 mS cm -1. Significantly less of nitrates was in lettuce grown in spring than in autumn. Higher levels of EC significantly increased the content of P and K in lettuce leaves during in both growing cycles, while the content of Ca and Mg was not significantly different. The applied substrates had a significant impact on the content of K, P, Ca and Mg in leaves of lettuce. The best concentration of the nutrient solution used for flood fertigation of greenhouse lettuce was EC 3.0 mS cm-1 for the spring cultivation and EC 4.0 mS cm-1 for the fall crop.
Chemical composition of shells of Cepaea vindobonensis (Férussac) from sites with carbonate and carbonate-silica bedrock in central and south-eastern Poland and from alluvial deposits of the Vistula River indicates a relation between the chemism of the environment in which the species occurs and the chemical composition of the shells. The ability to accumulate heavy metals in the shell of C. vindobonensis makes this species a potential bioindicator.
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