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Long-chain omega-3 fatty acids (n-3 FAs) influence meat tenderness, juiciness, and flavor, and are beneficial to human health. The percentage of long-chain n-3 FAs in total FAs is termed the omega-3 index (O3I). It is thus of great interest to favor rising this index in bovine skeletal muscle, to obtain healthier, tastier, and more nutritive meat. This study was aimed to detect transcriptomic variations related to O3I in muscles in 15-month-old males of 4 Spanish cattle breeds raised under the same conditions. Through the analysis of extreme O3I phenotypes, 3 genes of interest (AANAT, UCP2 and AHA1) were identified. AANAT and UCP2 were strongly up-regulated, while AHA1 was repressed in animals with a high 031. Moreover, gene expression differed between GDFS-null animal muscles (tested for nt821del11 and Q204X mutations) and the wild-type muscles for genes GDH1, IGF2R, FADS1, ASPH, and AIM1, all showing down-regulation in Asturiana de los Valles calves with muscle hypertrophy (GDF8-null). This shows that in GDF8-null animals other pathways are used for FA synthesis.
The experiment was carried out on 200 ROSS 308 broiler chickens that were randomly assigned to 5 equal groups (K, D1, D2, D3, D4). The birds were fed the mixtures Starter (1–21 days of rearing) and Grower/Finisher (21–42 days) oiled with a mixture of soybean oil (3%) and linseed oil (3%). Two different grains represented the experimental factor, and were added to the mixtures in a 1:1 ratio, according to the following design: group K (control) – maize and wheat, group D1 – wheat and barley, group D2 – wheat and triticale, group D3 – maize and barley, group D4 – maize and triticale. It was shown that chickens fed the mixtures with triticale at age 42 days had significantly (P ≤ 0.01) higher body weight than those that received mixtures with barley. In the whole period of rearing, a significantly (P ≤ 0.05) lower feed conversion ratio was found in chickens fed diets containing wheat and barley, as compared with the control group. The used mixtures did not influence musculature and the proportion of skin with subcutaneous fat in carcasses; however, a significantly (P ≤ 0.05) lower abdominal fat level was found in carcasses of chicken fed mixtures containing wheat and triticale and wheat and barley, as comparer with the broilers from other groups (K, D3 and D4). No effect of feeding on the content of most fatty acids in the lipids of leg muscles was found, while the breast muscles of the birds receiving the mixture with wheat and barley have shown a significant decrease in the proportion of linolenic acid and an increase in stearic acid. There were no significant effects of grain composition in mixtures in terms of muscle pH or sensory properties of the meat; they had, however, an impact on its color.
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