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To investigate the effects of feeding diets differing in the content and in the type of protein, fat and carbohydrates on feeding efficiency and serum cholesterol two experiments on male rats were carried out. In the first the animals were fed semipurified diets containing 8, 12 or 16% of energy from protein with the following ratio of energy from fats to carbohydrate (F/CHO): 1/1, 1/2 and 1/3. In the second experiment eight combined diets containing various sources of protein (casein or soybean concentrate), fat (lard of rapeseed oil) and carbohydrates (sucrose or wheat starch) were used. After 60 days of feeding it was found that feed efficiency, concentrations of serum total cholesterol (TC) and cholesterol in HDL fraction (HDL-C) significantly depended on the amount and source of protein in the diet. The ratio F/CHO and the type of consumed fat effectively modified the influence of dietary protein on serum cholesterol. Rapeseed oil produced significantly higher HDL-C concentration than lard. Dietary sucrose, compared with starch, did not influence feeding efficiency and serum cholesterol. There was a combined effect of fat source — carbohydrate source on body weight and weight/length index but not on the concentration of serum cholesterol in the rat.
Possibility of reduction of protein level in feed mixtures of similar content of amino acids digestible in the small intestine and its influence on fattener’s performance and nitrogen retention were determined. Thirty pigs (35 kg) were divided into 3 groups. The control animals were fed with diets with crude protein level suggested by polish pig feeding standards (Normy żywienia... 1993) – 17% in grower and 15% in finisher diet. In the experimental groups (I and II) the protein level was reduced about 10 or 20% respectively. No significant effect on feed intake, weight gains and feed utilization was found. The animals from the both experimental group utilized less crude protein (P=0.001) than in Control group. The significant (P=0.001) reduction in nitrogen excreted in urine and total N-excretion in both group with reduced protein level in feed mixtures, were observed. Nitrogen retention in g/day was higher for group I compared to other groups and N-retention : N intake were significantly higher (P<0.05) in both experimental groups. The reduction of crude protein level in the experimental diets did not affect slaughter performance but in group II the tendency to increase (P=0.064) in back fat thickness and decrease meat content in carcass, were observed. The results suggest that lowering the protein level in diets for high-meat crossbred pigs (Polish Large White × Polish Landrace) × (Hampshire × Pietrain) from 170 and 150 g kg⁻¹ to 136 g and 115 g kg⁻¹ in grower and finisher diets, respectively, but supplemented with synthetic amino acids, did not impact negatively production results of fatteners, but decreased nitrogen emission to the environment and cost of feed.
The average composition of amino acid profile of feed’s protein (0) was compared with the amino acid rumen undegraded protein (RUP), by calculating the distances of x² between each file of two proteins. The greater value of x², the greater difference between compared proteins. The biggest distance in cereals between 0 fraction and RUP was noted for rye (x² = 73.97), lower for barley (x² = 31.22) and the lowest for wheat (x² = 20.6). The composition of wheat AARUP is the closest to the AA content in feed. The differences in 0 protein fraction and RUP were of a little importance for soybean meal and for field bean (x² = 15.3 and x² = 23.4, respectively). Bigger distance level between protein fractions was noted for peas (x² = 81.2). Amino acid profiles of 0 fraction and amino acid profiles of RUP in hay of the Italian rye-grass and of the red clover were similar (x² = 46.5 and x² = 59.6, respectively), but they were considerably different from analogous protein fractions of meadow fescue and perennial rye-grass (x² = 144.5 and x² = 167.86, respectively).
Background. Reducing feed cost and the pollution originating from feed have been principal matters in fish nutrition. Mixed feeding schedules have been proposed as one of the methods to reduce both of them and successfully tested in many fish species. The aim of the present study was to determine the mixed feeding schedules′ suitability for culture of rainbow trout. Materials and Methods. Five different feeding schedules were tried by alternating the presentation of a low-protein (28.1%) diet (A) and a high-protein (49.9%) diet (B). The feeding schedules employed were 1A/1B, 1A/2B, 1A/3B, 2A/2B, and 2A/3B where the numerical values refer to the number of days each diet (A and B) was offered continuously. Fish given diets A and B continuously served as controls. Totally, seven dietary treatments were tested in triplicate for 10 weeks. Results. The fish maintained on the different mixed feeding schedules exhibited lower weight gain than those fed continuously on the high-protein diet (P < 0.05). The fish reared on schedule 1A/3B (1 day diet A alternated with 3 days diet B) had similar specific growth rate (SGR), feed conversation ratio (FCR), and protein efficiency ratio (PER) to those fed diet B continuously and was best among all mixed feeding schedules. However, the most cost-effective treatment was 2A/2B. In addition, this group compared to B resulted in significant increase and reduction of phosphorus retention and discharge, respectively (P < 0.05). The treatments significantly effected body moisture, fat- and ash levels (P < 0.05), but not protein. Conclusion. The study shows that mixed feeding schedules are promising for use in rainbow trout culture as they result in feed cost saving and reduced phosphorus discharge in the effluents.
Background. The African bagrid catfish, Chrysichthys nigrodigitatus (Lacepède, 1803), is an omnivorous species cultured in both fresh and brackish waters because of its good growth rate, excellent taste, and high market demand. There has been little nutritional work on this economically important species. This study was designed to determine the effects of varying dietary crude protein levels on growth, nutrient utilization and body composition of juvenile C. nigrodigitatus. Materials and Methods. One hundred and twenty fish with an initial mean weight of 21.33 ± 0.48 g were divided randomly among 12 tanks for triplicate groups that were fed diets containing 20%, 25%, 30%, and 35% crude protein (CP). Feed consumption and fish weight were measured biweekly for eight weeks, after which proximate composition of the fish was determined. Results. Fish fed the 35% CP diet had the best growth performance and nutrient utilization (P < 0.05), with a mean weight gain of 88.3% ± 0.2%, feed conversion ratio (FCR) of 2.60 ± 0.12, and protein efficiency ratio (PER) of 1.10 ± 0.05. The least weight gain (32.1% ± 0.9%) and poorest FCR and PER (5.70 ± 0.06 and 0.87 ± 0.01, respectively) were observed in fish fed the 20% CP diet. Body protein content was not affected by diet, but body lipid increased significantly (P < 0.05) with increasing dietary protein levels. Conclusion. C. nigrodigitatus juveniles will grow best when fed diets containing at least 35% CP.
The aim of the study was to evaluate the influence of feeding goats diets with different protein levels (11.4, 13.3 and 16.9%) and a small change of fatty acid contents on the concentrations of trans11C18:1 and conjugated linoleic acid (CLA) isomers in milk. The experimental design was 3 × 3 Latin Square. Each 22-day experimental period consisted of 14 days adaptation to the diets and 8 days for milk samples collection. Separation of methylated CLA isomers was achieved using GLC. Milk production was nearly the same when the low- and medium-protein diets were fed, while the high-protein diet resulted in a tendency to increase milk production. The concentrations of atherogenic (A-SFA), thrombogenic (T-SFA) and total saturated fatty acids (SFA) were similar in milk from goats fed the diets containing low- and medium-protein contents, whereas the diet containing the highest protein content resulted in an increase in the daily production of SFA and A-SFA in milk. The concentration and daily production of cis9trans11CLA, trans10cis12CLA, the sum of CLA isomers and usually trans11C18:1 and the sum of trans,transCLA isomers in milk increased as the dietary protein level increased. The higher dietary protein content resulted in the decrease in the concentration ratio of SFA and unsaturated fatty acids (UFA) (SFA/UFA) and tended to the increase in the capacity Δ9-desaturation.
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