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The study was conducted on 36 female Pharaoh quails (3 groups, 12 birds per group). The experiment covered the 7th to the 20th weeks of birds’ lives. The control group (I) received standard feed formulated for adult quails. Groups II and III received standard feed with 4% and 7% of amaranth seeds, respectively. All feeds were isoproteinous and isocaloric. At the age of weeks 20, 12 females were randomly selected from each group and slaughtered. In the isolated breast and leg muscles water-holding capacity, thermal drip, colour, basic chemical composition, fatty acids profile were determined and sensory evaluation performed. Amaranth seed supplementation showed no effect on the basic chemical composition of breast and legs muscles and fatty acids profile. Deterioration in the flavor of cooked quail breast muscle was observed with the higher dosage of the seeds in the diet.Improved muscle tenderness was observed in birds receiving 4% of amaranth seeds in the diet.
The aim of the study was to determine the effect of linseed and rapeseed dietary supplementation on the fatty acids profiles of two ostrich fat depots: breast and subcutaneous (above the leg). The study was carried out on 40 ostriches raised in five groups – control (C) or with 4% (L4) or 8% (L8) linseed, or 5% (R5) or 10% (R10) rapeseed in the diet, from hatching to 12 months of age. Fat samples of breast (BF) and leg fat (LF) were taken for fatty acids analysis. Generally ostrich fat has high contents of PUFA (BF – 23.9, LF – 20.2 g/100 g FAME), especially linoleic acid (BF – 16.4, LF – 12.5 g/100 g FAME) and linolenic acid (BF – 5.7 and LF – 6.2 g/100 g FAME). Ostrich BF had a higher content of n-6 FA and total PUFA and lower n-3 FA than the LF. Both fat depots had desired PUFA/SFA ratios above 0.4, but not desirable n-6/n-3 ratios. BF had significantly higher (0.69)PUFA/SFA ratio than LF (0.55). Both L4 and L8 caused higher total PUFA content (27.8, 25.6 g/100 g FAME, respectively) and higher PUFA/SFA ratios (0.74, 0.75, respectively) and lower n-6/n-3 ratios (1.5, 1.8, respectively) compared to C. The rapeseed supplementation decreased the LA content in ostrich fats (R5- 14.1, R10-13.4g/100g FAME), causing a lower n-6/n-3 (4.1, 4.6, respectively) ratio compared to C (6.1). The supplementation of ostrich diets with linseed improved the nutritional value of ostrich fat by increasing the n-3 FA, total PUFA content and PUFA/SFA ratio. Although the leg fat had a lower PUFA content, both depots of ostrich fats can be recommended as valuable ingredients for value-added meat products fit for human consumption.
Growth performance parameters and selected quality traits of meat and femoral bone of broiler chickens fed diet supplemented with amorphous diatomaceous earth. This study was aimed at analyzing the effect of the addition of amorphous diatomaceous earth to feed on growth performance parameters and selected quality traits of meat and femoral bone of broiler chickens. The study was conducted with 60 fast-growing Ross 308 broiler chickens, reared until 42 days of age and divided into a control group (C) and two experimental groups (D2 and D4) (20 birds each). The diatomaceous earth (diatomite) was administered to the birds’ feed from the groups D2 - 2%, D4 - 4%. Individual body weight, feed intake and mortality of chickens were controlled. On day 42 of rearing, 6 males were selected from each group for slaughter followed by dissection. Dressing percentage, content of muscles and giblets (gizzard, liver and heart), were calculated, and in samples of breast and leg muscles the chemical and physicochemical properties were analyzed. Resistance of the femoral bone to fractures was determined. The addition of diatomite did not affect the health status of chickens. Significantly higher body weight at 42 days of rearing was noted in the group C vs D4 (P ≤0.05). Significantly higher (P ≤0.01) content of pectoral muscles and lower (P ≤0.05) fat in the carcass of D2 vs C were noted. The addition of diatomaceous earth did not affect the chemical composition of the breast muscles. The fat content in leg muscles was significantly reduced (P ≤0.05) and water content was increased (P ≤0.05) in D2 vs C. Femoral bones of D4 birds were significantly more resistant (P ≤0.05) to breaking than in C. Direct relationship between the amount of diatomite and the strength of the femur was found. The most optimal supplementation was considered as 2%.
Forty ostriches were raised in five groups [control (C) or with 4% (L4) or 8% (L8) linseed, or 5% (R5) or 10% (R10) rapeseed in the diet]. Linseed supplementation (L4 and L8) improved the nutritive value of the ostrich meat by increasing (P<0.001) the α-linolenic acid content (>4.2%FAtotal) and PUFA/SFA ratio (>1.0) as compared with the control group (1.7% FAtotal and <0.94, α-linolenic acid and PUFA/SFA, respectively), whereas the effect of rapeseed was lower (2.2%, and <0.99, α-linolenic acid and PUFA/SFA, respectively). Dietary treatment lowered (P<0.001) the n-6/n-3 ratio from ca. 11 in the C group to 4 in the L8 and L4 groups. Overall, the results indicate that inclusion of linseed into ostrich diets has a positive effect on the fatty acid composition, allowing the production of meat enriched with n-3 fatty acids
The aim of this study was to determine the effect of fish-based feed materials, as a source of readily available protein contained in fish broth and essential polyunsaturated fatty acids (PUFAs) found in fish oil, on the health of piglets and rearing results. The experiment was conducted on a commercial pig fattening farm. The study involved a total of 80 weaners with an approximate body weight of 15 kg. The experiment was carried out over a period of 40 days. Feed samples were subjected to laboratory analyses. Blood samples were collected from experimental group animals to determine serum biochemical and immunological parameters. The body weight gains of weaners, mortality rates and average feed intake per animal were calculated for the entire experimental period. The addition of fish broth and fish oil significantly improved the n3:n6 fatty acid ratio in diets. The presence of EPA and DHA in the experimental diet could have had a positive health effect on piglets, comparable with that exerted by therapeutic doses of zinc often administered to pigs of this age group. During the experiment, feed conversion ratio (FCR) gain was considerably reduced in the experimental group, with similar daily gains in the control and experimental group.
The effect of L-carnitine supplementation during gestation and lactation on the performance parameters of sows was studied. The trial comprised a total of 73 sows that were divided into the control and treated groups; each was fed diets with and without supplemental L-carnitine during pregnancy (0 vs. 125 mg L-carnitine daily/sow) and lactation (0 vs. 250 mg L-carnitine daily/sow). L-carnitine supplementation resulted in the numerical increase of sow body weight at weaning (215.2±5.4 vs. 205.0±5.0, LSM±Se, p≥0.05), with a significantly expressed effect on sows born in 1998 (227.1±8.4 vs. 193.6±9.2, LSM±Se, p=0.01). There was a numerical increase in average number of total born and viable piglets per litter in treated sows (11.0±2.0 vs. 10.7±1.9, LSM±Se, p≥0.05; 10.8±2.1 vs. 10.4±1.9, LSM±Se, p≥0.05 respectively), although the tendency towards increasing the same parameters was determined in sows born in 1998 (11.00±0.54 vs. 9.82±0.59, LSM±Se, p=0.14; 10.9±0.6 vs. 9.5±0.6, LSM±Se, p=0.09). The total number of piglets which were smaller than 800g at birth tended to be lower (0.9±1.2 vs. 1.4±1.6, LSM±Se, p=0.08) and significantly different in sows born in 1998 (0.9±0.4 vs. 2.5±0.4, LSM±Se, p=0.01). The number of piglets fit for rearing was influenced by L-carnitine supplementation only in the sows born in 1998 (10.8±0.5 vs. 9.1±0.6, LSM±Se, p=0.03). The litter weight was not influenced by L-carnitine supplementation.
The aim of the present study was to determine the effect of microbial phytase addition to sow diets on a mineral content, mineralization level and geometric parameters of femoral and humeral bone. The studies were done on 75 sows assigned to 3 feeding groups. The animals from group I (positive control) were fed a diet of standard calcium and phosphorus dietary contents which complied with the requirements of the Polish Norms for Pig Nutrition (1993). The sows from group II (negative control) received a diet without an inorganic phosphorus content and finally, group III was provided with a diet without an inorganic phosphorus additive, but supplemented with microbial phytase (500 PU kg-1) and formic acid. After lactation completion and piglet weaning, 4 sows were selected from each group for slaughter and laboratory evaluation of femoral and humeral bone samples. The bone samples were examined for a content of dry matter, crude ash and minerals (phosphorus, Ca⁺², Mg⁺², Mn⁺², Zn⁺², Cu⁺²). The isolated femurs were analyzed for a mineralization degree and geometric parameters. A combined microbial phytase with formic acid supplementation significantly increased manganese and zinc concentration in femoral bone and a level of phosphorus, calcium, zinc and iron in humeral bone of sows. There was also observed significantly higher trabecular bone mineral density (Td) in the femoral bone as well as the bone volume. The evaluation of geometric parameters and bone cortical indices showed a significant influence of the sow feedstuff supplementation with microbial phytase and formic acid on the parameters studied.
Diets supplemented with Mytilus galloprovincialis from polluted and non-polluted waters and their influence on zinc content in liver of rats loaded with cholesterol. Mussels may reflect the environmental problems that the ecosystem faces. Mytilus galloprovincialis is widely used as an indicator of water pollution in biological monitoring studies. These mussels are filter feeders and may accumulate important ecosystem pollutants what in turn may pose a potential risk to other organisms in the food chain. The aim of the study was to determine zinc content in the diets supplemented with mussels from polluted and non-polluted areas and in the livers of rats fed these diets for 30 days. The zinc content in the diets with mussels from contaminated and noncontaminated waters and in rat livers was examined using a flame atomic absorption spectrophotometry (Perkin-Elmer 1100B). The slight increase in zinc content from 73.2 ±1.2 mg/ /kg in ch diet (control with 1% of cholesterol) to 77.2 ±0.5 mg/kg in the diet with mussels from polluted site was found, but zinc content in all diet was within the normal limits reviewed by McDowell (1992) – 40 to 100 ppm. Zinc content in the diet supplemented with mussels from nonpolluted site (73.7 ±3.7 mg/kg) was similar to that found in ch diet. It can be concluded that cholesterol does not affect zinc bioaccumulation. However, prolonged consumption of mussels from contaminated sites may increase accumulation of zinc in the liver of rats.
Rats were offered ad libitum zinc in twice the level of the standard diet (23 mg/kg diet), and bentonite (2% additive) for 28 d, together with traces of zinc chloride (labelled with zinc-65) given intragastrically. Results provided evidence that bentonite increased the body retention of zinc in comparison to the diet without this agent. Furthermore, the addition of bentonite did not influence feed intake, organ to body ratios, and haematological values, although a visible decrease in body weight gains following bentonite feeding was noted. These findings may be useful when bentonite fertilised diet is provided to animals pastured in areas with higher zinc levels.
Autism is a neurodevelopmental disorder with symptoms arising that are apparent throughout the patient’s lifespan. Autism Spectrum Disorders (ASD) are characterised by impaired social and communication interactions as well as restricted, repetitive interests and behaviour. Currently in Poland, about 50 000 people suffer from autism, of which 1/5 are children. Epidemiological studies show that the incidence of autism is increasing, which may be due to the diagnostic category of ASD having been developed. Of vital importance in the treatment of autism, is early diagnosis which is conducive to more rapidly improving the quality of patients’ health. It is believed that both genetic and environmental factors may affect the development of the disease. Moreover, expert opinion emphasises the importance of making an adequate diagnosis when the first symptoms of autism start appearing which can be both psychological, gastro-intestinal and metabolic ones. Conventional treatment is based on the combination of behavioural and dietary therapy together with pharmacotherapy. For example, adapting an appropriate diet could help alleviate the disease severity, as well as the psychological and gastrointestinal symptoms. Much scientific research has indicated that pathogenesis of autism may have a beginning already in foetal life. During pregnancy, specialists should take special heed of metabolic disorders, which can increase the risk of ASD in children. One of the dietician’s tasks are to properly assess the nutritional status of mothers before and during pregnancy, thereby allowing changes in nutrition to be made wherever necessary in order that metabolic indicators be improved. Thus an important part of autism therapy is the improving patient’s nutritional status to prevent the onset of gastrointestinal symptoms. Adopting diets and tailored to individual disease symptoms, is linked to the nutritional requirements and food preferences of the patient. Specialists also emphasise that continual monitoring of the diet and nutritional status of children with ASD is required. It is also essential to start adequate dietary management in autistic patients with overweight, obesity or wasting, caused by improper nutrition. Frequently only a dietary therapy is insufficient to effectively treat autism. Many studies demonstrate the need to supplement the nutritional deficiencies of autistic patients with fatty acids omega-3, probiotics, vitamins and minerals in combination with medical and psychological interventions. A properly designed elimination diet adapted to the patient’s individual may also lead to relief of the autism symptoms and the occurrence of gastrointestinal disorders. Parents and caregivers should therefore be aware of the benefits of nutritional therapy and need for proper monitoring the treatment of patients with ASD. A review of nutritional factors, dietary treatments and diet supplementation in patients with ASD is presented.
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