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The role of Cryptosporidium parvum and rotaviruses in diarrhoea of infants

100%

Spiewak E., Malafiej E., Wierzbicka E., Zieba M.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

2

Providencia alcalifaciens as the presumptive cause of diarrhoea in dog

75%

Krol J., Janeczek M., Pliszczak-Krol A., Janeczek W., Florek M.

Polish Journal of Veterinary Sciences

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2007

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tom 10

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nr 4

A case of diarrhoea in a four-month-old golden retriever is described. On the basis of anamnesis and bacteriological examination, the diagnosis of bacterial enteritis due to Providencia alcalifaciens was reached. Although there are contradictory opinions about the role of this organism as the enteric pathogen, it seems that Providencia alcalifaciens should also be taken into consideration in the routine bacteriological diagnostics of diarrhoea in dogs.

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Structural analyses of Shigella invasion proteins reveals non-conserved; intrinsically unstructured regions

75%

Chakrabarti S., Ganguli S.

International Letters of Natural Sciences

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2013

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tom 05

Shigella is one of the most common bacterial pathogens that are isolated from patients with diarrhea. Various attempts are being made worldwide with encouraging observations; still the emergence of multidrug-resistant Shigella strains and a continuous high disease incidence imply that shigellosis is an unsolved global health problem which can probably be solved only by developing a proper vaccine and a vaccine regime for the disease. The need of the hour is to foster the development of an effective vaccine which should not only serve to improve hygiene but also should be able to curb infections by the pathogen. This goal can only be achieved by gaining proper detailed knowledge underlying Shigella pathogenesis. The analyses of the Shigella invasion proteins which have been long been targeted to be potential candidate vaccines remains an open ended problem and forms the core of this present computational study which identifies the fact that long regions in the structure of the proteins are disordered having no distinct structural conformation; multiple alignments however, did not show any conserved stretches in the disordered regions. The results probably explain the ability of these proteins to interact with multiple cellular proteins and perform a diverse array of functions leading to successful pathogenesis.

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Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves

75%

Matsuura Y., Matsubayashi M., Nukata S., Shibahara T., Ayukawa O., Kondo Y., Matsuo T., Uni S., Furuya M., Tani H., Tsuji N., Sasai K.

Acta Parasitologica

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2017

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tom 62

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nr 1

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Relationship between the degree of dehydration and the balance of acid-based changes in dehydrated calves with diarrhoea

75%

Guzelbektes H., Coskun A., Sen I.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 1

The aim of the study was to investigate acid-base changes and to determine some serum biochemical parameters in dehydrated calves with diarrhoea according to the degree of dehydratation. Thirty diarrhoeic dehydrated calves submitted to treatment in the university clinic, were used in the study. The calves were accessed as suitable for this study, if they were moderately or severely dehydrated according to clinical symptoms. All sick calves had the usual yellow and watery diarrhoea. Calves with 4% to 8% dehydration (moderate) had a weak suckle reflex, dry mucous membranes, warm mouth and partly good muscular tone. Calves with 10% and above dehydration (severe) were unable to stand, and had no suckling reflex and cold mouth. The mean pH, base excess, chloride (only severely dehydrated group) and sodium were -significantly decreased in both moderately and severely dehydrated groups. On the other hand, potassium, phosphorus, HCO₃⁻ and anion gap levels were increased, compared to that of the control group. Results of this study showed that there was a relationship between the base excess and anion gap, with a degree of clinical dehydration. However, there may not be an exact correlation between the degree of dehydration and the severity of acidosis. Based on the clinical symptoms, the results of this study could be a useful tool under field conditions, in estimating the base excess in diarrhoeic dehydrated calves, when acidosis therapy is needed.

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Genetic identification of virulence markers in shiga toxin-producing Escherichia coli [Stec] strains

75%

Osek J.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

E. coli strains isolated from cattle (7 strains) and from children with diarrhea (7 strains) were tested for shiga toxin (stxl and stx2), intimin (eae), and enterohemolysin (ehly) virulence marker genes using PCR. Moreover, the eae genes were further classified by determination of α, β, δ, and ε variants. It was shown that all strains possessed the eae and ehly genetic determinants, as well as genes for stxl (3 strains), stx2 (7 strains) or both toxins (4 strains). PCR amplifications performed with eae-specific primers showed that all 12 O157 isolates were eae γ-positive whereas two strains of O26 serogroup harboured the eae β gene. The obtained results demonstrated that shiga toxin-producing (STEC) E. coli of human and cattle origin isolated in Poland possessed very similar or identical virulence markers.

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Research of pathomechanism and therapy of Colitis X

75%

Nicpon J., Czerw P., Zawadzki W.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2000

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tom 03

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nr 2

During the conducted experiments the authors aimed at an explanation of pathogenesis of diarrheal disease of horses, called Colitis X. The effort of finding the most effective therapy and prevention of the disease was also undertaken.

8

The presence of free shiga-like toxins in stool specimens of patients with diarrhea during one year study

75%

Sobieszczanska B. M., Kuzko K., Gryko R., Dobrowolska M.

Polish Journal of Microbiology

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2005

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tom 54

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nr 1

During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures of strains isolated from the two positive stool samples were negative for Stxs-production in Vero cytotoxicity assay and by PCR.

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Incidence and clinical aspects of colon ciliate Buxtonella sulcata infection in cattle

75%

Tomczuk K., Kurek L., Stec A., Studzinska M., Mochol J.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 1

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Diagnostics of cryptosporidiosis - own experience

75%

Grzeszczuk A., Kalinowska A.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

11

Protection of piglets against diarrhoea by commercial rotavirus - E.coli vaccine

75%

Sereika V., Salomskas A., Mazeika A.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

12

RT-PCR in diagnosis of BVDV infection

75%

Polak M. P., Zmudzinski J. F.

Bulletin of the Veterinary Institute in Puławy

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1999

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tom 43

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nr 2

RT-PCR was evaluated as a diagnostic tool for BVDY detection. First, five sets of primers were checked for their diagnostic ability to amplify the number of field isolates. Only primers within the 5'UTR region amplified all field isolates as well as all reference laboratory strains. Next, sensitivity of the test was evaluated. This was equal to 1 TCID₅₀ of the virus. lt was concluded that RT-PCR can be used as an alternative diagnostic test for inconclusive results of virus isolation in cell culture especially for the noncytopathic strains. However, high cost of equipment and reagents for the test precludes it as a routine diagnostic method except for research applications.

13

Investigation of bovine rotavirus 12M strain as a candidate for a vaccine

75%

Salomskas A.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

14

Effectiveness of zinc oxide in prophylaxy of post-weaning diarrhoea of pigs

75%

Pejsak Z., Markowska-Daniel I., Mokrzycka A., Szkoda J., Rutkowska-Pejsak B.

Polish Journal of Veterinary Sciences

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1998

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tom 01

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nr 2

The efficacy of zinc application in the treatment and prevention of diarrhoea in the weaning period of piglets has been demonstrated. However, beside this positive effect some authors suggested the possibility of harmful effects of zinc oxide supplementation on the environment and public health. The aims of the study were to evaluate, in experimental and field conditions, the influence of different doses of ZnO on productivity, losses and body weight gain (b.w.g.) as well as the Zn level in different tissues of pigs. During the first stage of the study three groups of weaned piglets were fed high doses (2 500, 3 000 and 3 500 ppm) of ZnO for 7 days prior to and 14 days after weaning. The best results were observed in the group fed with highest doses of ZnO, in this group daily b.w.g. was 26 grams higher in comparison with the controls. Tissue samples from muscles and selected organs (liver, kidneys, pancreas, heart and brain) were taken from experimental pigs at slaughter after the animals had reached a body weight of about 100kg. The results of toxicological examination indicated that 14-days of treatment with high doses of ZnO have no influence on zinc concentration in tissues. Field experiments aimed at safety and evaluation of the usefulness of ZnO on the prophylaxy of post weaning diarrhoea, performed in 8 farms, demonstrated reduction of diarrhoea symptoms from about 48 to 15%. In summary, it may be concluded that short-term application of ZnO in prevention of post weaning diarrhoea is efficient for pigs and does not influence zinc concentration the fatteners tissues.

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Insertion element IS3 PCR-based method for molecular analysis of enterotoxigenic Escherichia coli isolated from suckling piglets with diarrhoea

75%

Weiner M., Dacko J., Osek J.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

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Cellular identification and in silico characterization of protein phosphatase 2C (PP2C) of Cryptosporidium parvum

63%

Gomez-Sandoval J. N., Okhuysen P., Mondragon-Flores R., Escalona-Montano A. R., Aguirre-Garcia M. M.

Acta Parasitologica

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2020

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tom 65

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nr 3

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Isolation, biotyping, and serotyping of Yersinia enterocolitica strains from fattening pigs

63%

Bancerz-Kisiel A., Szczerba-Turek A., Platt-Samoraj A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

The study has been taken up to collect more data on Yersinia enterocolitica isolated from pigs as the main reservoir and source of infection with strains pathogenic for humans. Bacteriological examinations, bio- and serotyping were conducted on 616 rectal swabs, taken from 308 fattening pigs. Two samples were taken from each animal to determine the ability of Y. enterocolitica to grow under different temperature conditions (warm and cold culture). It has been proven that low temperature constitutes a suitable culture condition. 138 Yersinia enterocolitica strains were isolated (22.40%), most of which (65.22%) were obtained in cold culture and 99.28% included in biotype 3 (one strain belonged to biotype 2). Serotyping yielded a positive result in 107 strains with the diagnostic serum for antigen O:3, in 18 - with the serum for antigen O:9, and 13 strains were determined to be non-typable. The results indicated that asymptomatic infections with Y. enterocolitica strains of the biotypes and serotypes pathogenic for humans are common in pig population.

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COVID-19 - more than respiratory disease: a gastroenterologist’s perspective

63%

Konturek P. C., Harsch I. A., Neurath M. F., Zopf Y.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 2

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Dog’s genotype of Giardia duodenalis in human: first evidence in Europe

63%

Strkolcova G., Madar M., Hinney B., Goldova M., Mojzisova J., Halanova M.

Acta Parasitologica

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2015

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tom 60

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nr 4

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First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan

63%

Matsubayashi M., Takayama H., Kusumoto M., Murata M., Uchiyama Y., Kaji M., Sasai K., Yamaguchi R., Shibahara T.

Acta Parasitologica

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2016

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tom 61

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nr 2

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