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Caveolin-1 localization in chicken embryo chorioallantoic membrane treated with diamond and graphite nanoparticles

100%

Wierzbicki M., Sawosz E., Grodzik M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

2012

tom 51

Caveolin--1 is a multifunctional protein and major component of caveolae membranes that participates in regulation of signaling pathways, endocytosis and molecular transport. Caveolin-1 takes part in regulation of angiogenesis regulation signaling pathways. Diamond nanoparticle have been shown to inhibit development of blood vessel. Molecular mechanism of diamond nanoparticles anti-angio-genic activity can be related with interactions with cellular membranes. The objective of this experiment is to verify effect of carbon nanoparticles on morphology of highly vascularized chicken embryo chorioallantoic membrane (CAM) and caveolin-1 intracellular localization. In this study two types of carbon nanoparticles were used: diamond nanoparticles (ND) and graphite nanoparticles (NG), which are similar in size (3-5 nm) but different in molecular structure. At day six of chicken embryo embryonic development sterile implant of diameter 10 mm made from Waterman filter paper ware placed on chicken embryo CAM. At day seven of embryonic development implants with CAM were subjected to further analyzes. CAM cross-sections were immuno-localized with anti caveolin-1 antibody and visualized by confocal microscope. Three dimensional analysis of chorion membranes show that ND, but no NG change intracellular distribution of caveolin-1. Furthermore ND decreases density of mesenchymal cells and extracellular matrix collagen fibers

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Analysis of a fusion between rat glioma cells and biomimetic liposomes with encapsulated diamond nanoparticles or curcumin

100%

Strojny B., Grodzik M., Sawosz E., Gromadka M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

2016

tom 55[2]

Analysis of a fusion between rat glioma cells and biomimetic liposomes with encapsulated diamond nanoparticles or curcumin. Liposomes are used as carriers for different bioactive agents, both hydrophilic, which are encapsulated in water core of the liposome, and hydrophobic, which are entrapped within liposome walls. The walls are built from phospholipid bilayer, therefore their structure resembles cell membrane. It was hypothesized that if the wall is made of set of lipids typical for a cell, the liposome will be eagerly consumed by the cell. We performed the experiments using C6 rat glioma cells as an example, since central nervous system cells are extremely rich in lipids, including the unique ones. Since all cancer cells have high proliferation potential, they need to absorb precursors to build cell membrane around new cells, therefore such biomimetic liposomes may be one of the most effective way to deliver anticancer agents into the cell. Analysis of physicochemical properties of obtained liposomes, as well as in vitro tests, showed that obtaining such liposomes is possible and that the liposomes are biocompatible, stable carrier both for hydrophilic and hydrophobic agents. Encapsulation of diamond nanoparticles did not affect the liposomes, whereas entrapping of curcumin, which is a spice known in traditional Asian medicine for its anticancer properties, significantly increased its activity. Obtained results showed that biomimetic liposomes can be effective, individually-tailored carriers for bioactive agents.

Ograniczanie wyników

2 Annals of Warsaw University of Life Sciences - SGGW. Animal Science

2 Grodzik M.

2 Sawosz E.

1 Gromadka M.

1 Strojny B.

1 Wierzbicki M.

1 2016

1 2012

Wyniki wyszukiwania

Caveolin-1 localization in chicken embryo chorioallantoic membrane treated with diamond and graphite nanoparticles

Analysis of a fusion between rat glioma cells and biomimetic liposomes with encapsulated diamond nanoparticles or curcumin