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Selective splitting of 3'-adenylated dinucleoside polyphosphates by specific enzymes degrading dinucleoside polyphosphates

100%

Guranowski A., Sillero A., Gunther-Sillero M. A.

Acta Biochimica Polonica

2003

tom 50

nr 1

Several 3' -[32 P]adenylated dinucleoside polyphosphates (NpnN' p*As) were synthesized by the use of poly(A) polymerase (Sillero MAG etal., 2001, Eur JBiochem.; 268: 3605-11) and three of them, ApppA[ 32P]A or ApppAp*A, AppppAp*A and GppppGp*A, were tested as potential substrates of different dinucleoside polyphosphate degrading enzymes. Human (asymmetrical) dinucleoside tetraphosphatase (EC 3.6.1.17) acted almost randomly on both AppppAp*A, yielding approximately equal amounts of pppA + pAp*A and pA + pppAp*A, and GppppGp*, yielding pppG + pGp*A and pG + pppGp*A. Narrow-leafed lupin (Lupinus angustifolius) tetraphosphatase acted preferentially on the dinucleotide unmodified end of both AppppAp*A (yielding 90% of pppA + pAp*A and 10 % of pA + pppAp*A) and GppppGp*A (yielding 89% pppG + pGp*A and 11% of pG + pppGp*A). (Symmetrical) dinucleoside tetraphosphatase (EC 3.6.1.41) from Escherichia coli hydrolyzed AppppAp*A and GppppGp*A producing equal amounts of ppA + ppAp*A| and ppG + ppGp*A, respectively, and, to a lesser extent, ApppAp*A producing pA + ppAp*A. Two dinucleoside triphosphatases (EC 3.6.1.29) (the human Fhit protein and the enzyme from yellow lupin (Lupinus luteus)) and dinucleoside tetraphosphate phosphorylase (EC 2.7.7.53) from Saccharomyces cerevisiae did not degrade the three 3 -adenylated dinucleoside polyphosphates tested.

Fragile histidine triad (FHIT) gene is overexpressed in colorectal cancer

80%

Wierzbicki P. M., Adrych K., Kartanowicz D., Dobrowolski S., Stanislawowski M., Chybicki J., Godlewski J., Korybalski B., Smoczynski M., Kmiec Z.

Journal of Physiology and Pharmacology. Supplement

2009

tom 60

nr 4

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Physiology and Pharmacology. Supplement

1 Adrych K.

1 Chybicki J.

1 Dobrowolski S.

1 Godlewski J.

1 Gunther-Sillero M. A.

1 Guranowski A.

1 Kartanowicz D.

1 Kmiec Z.

1 Korybalski B.

1 Sillero A.

1 Smoczynski M.

1 Stanislawowski M.

1 Wierzbicki P. M.

1 2009

1 2003

Wyniki wyszukiwania

Selective splitting of 3'-adenylated dinucleoside polyphosphates by specific enzymes degrading dinucleoside polyphosphates

Fragile histidine triad (FHIT) gene is overexpressed in colorectal cancer