Wyniki wyszukiwania

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Physical restraints in applying image analysis to quantitative studies

100%

Lecybyl R. H., Bilski B. I., Kazmierski M., Filipiak K., Jaroszewski J., Warchol W.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

2

Densitometric quantification of DNA topoisomers in ethidium bromide-stained agarose gels and chemiluminescence-detected X ray films

86%

Topcu Z.

Acta Biochimica Polonica

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2000

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tom 47

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nr 3

This study investigates the contribution of deformational strain imposed by topological interconversions of DNA in ethidium bromide-binding on agarose gels. Closed-circular plasmid DNAs were nicked using UV exposure and the DNA bands were quantified by densitometry. The results show that the closed circular DNA binds the same amount of the dye as its nicked counterpart. The relationship between the band intensity on X-ray films of chemiluminescence-detected Southern blots and DNA concentration was shown to be linear.

3

Effects of Schistosoma mansoni infection on lutein and beta-carotene concentrations in Biomphalaria glabrata snails as determined by quantitative high performance reversed phase thin-layer chromatography

72%

Dieterich N., Fried B., Sherma J.

Acta Parasitologica

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2013

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tom 58

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nr 3

High performance thin-layer chromatography was used to determine the concentration of β-carotene and lutein in the whole body and digestive gland-gonad complex (DGG) of uninfected Biomphalaria glabrata snails and those infected with Schistosoma mansoni for 6 and 8 weeks. Pigments were extracted from the snails using acetone and separated on EMD Millipore reversed phase C-18 plates with concentration zone using petroleum ether-acetonitrile-methanol (1:1:2) mobile phase. After development, two yellow pigment zones, lutein and β-carotene, were identified with respective R f values of 0.55 and 0.13 and then quantified by densitometry. Statistical analysis of the weight percentages of each pigment showed a significant decrease (P < 0.05) in the concentration of β-carotene in the DGGs of infected B. glabrata at 6 and 8 weeks post-infection compared to the uninfected snails. No significant differences were seen in the concentrations of β-carotene in the whole body of the uninfected versus infected snail samples. Changes in the lutein concentration of the infected DGG and whole snail bodies were insignificant compared to the uninfected controls. In conclusion, larval S. mansoni infection caused a significant decrease in the β-carotene concentration of the DGG at 6 and 8 weeks post infection.

4

Analysis of chosen organic tobacco smoke components and their metabolites by planar chromatography

72%

Tyrpien K.

Polish Journal of Environmental Studies

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2006

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tom 15

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nr 4

Planar chromatography was used for one of the final steps of multistage analysis of chosen organic compounds (polycyclic aromatic compounds, nicotine and their derivatives) occurring in tobacco smoke as well as their transformation products. These investigations present various development and detection modes, as well as the possibility of new stationary phase O30 application. The application of planar chromatography has enabled the identification and quantification of many organic pollutants dangerous for human health and the assessment of chosen people groups exposed to tobacco smoke living in the region of Upper Silesia in Poland.

5

Influence of snail meat in the diet on mandibular bone loss in male rats: a densitometric, tomographic and morphometric study

58%

Bienko M., Radzki R. P., Wolski D., Debiak P., Szkucik K., Ziomek M., Gondek M.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 11

The study was aimed at determining the effects of diets containing snail meat as the sole protein source, on mandible quality in male Wistar rats. In the experiment, three different snail-based diets were tested and compared with a casein-based control diet. These included snail meat from Helix pomatia, Cornu aspersum maxima and Cornu aspersum aspersum. In all diets, the protein content amounted to 10% (as calculated on a dry weight basis). Forty male Wistar rats with an initial body mass of 50 g ± 2 were randomly allocated to one control and three experimental groups. After 28 days of experimental feeding, the rats were sacrificed. Their mandibles were isolated and investigated by densitometric (DXA), tomographic (pQCT) and morphometric methods. Moreover, the mechanical parameters (ultimate strength and Young’s modulus) of the mandibles were measured. The results revealed that snail meat as the sole source of protein significantly decreased the bone mineral density (BMD) and content (BMC) of the mandibles. Moreover, the tomographic analysis demonstrated that each type of snail-based diet had a negative influence on the bone cortical and trabecular compartments (which was especially noticeable in the decreasing pQCT parameters). The investigation of mechanical resistance of the mandibles also revealed lower values of the ultimate strength and Young’s modulus in the snail-based diet groups, compared with the casein group.

6

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The differential impact of a density of Polish pine wood on cutting forces with its origin region taken into consideration

58%

Chuchala D., Orlowski K., Sandak A., Sandak J.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2013

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tom 82

7

Localization of ginsenosides in Panax quinquefolium root tissues

58%

Ludwiczuk A., Weryszko-Chmielewska E., Wolski T.

Acta Agrobotanica

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2006

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tom 59

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nr 2

We carried out histochemical studies to find the localization of ginsenosides in roots of Panax quinquefolium cultivated in Poland. We performed an anatomical study on the structure and localization of secretory canals on the cross section of 4-year-old American ginseng roots. We observed the occurrence of large secretory canals, mainly in the middle part of the secondary cortex and less in the phloem layer. In our studies, moreover, we demonstrated the production of secretory canals within the periderm layer. After the anatomical study, the 4-year-old ginseng root was divided into periderm, cortex and xylem, and the ginsenosides were extracted from each part of the root. The TLC separation of ginsenosides was performed on silica gel Si60 glass plates with chloroform-methanol-ethyl acetate-water-hexane, 20+22+60+8+4 (v/v) as mobile phase. Quantitative analysis of ginsenosides was performed by using the TLC-densitometric method. Concerning the distribution of ginsenosides in the different anatomical parts of the root of Panax quinquefolium, they were contained in the periderm layer at the highest level.

Ograniczanie wyników

Physical restraints in applying image analysis to quantitative studies

Densitometric quantification of DNA topoisomers in ethidium bromide-stained agarose gels and chemiluminescence-detected X ray films

Effects of Schistosoma mansoni infection on lutein and beta-carotene concentrations in Biomphalaria glabrata snails as determined by quantitative high performance reversed phase thin-layer chromatography

Analysis of chosen organic tobacco smoke components and their metabolites by planar chromatography

Influence of snail meat in the diet on mandibular bone loss in male rats: a densitometric, tomographic and morphometric study

The differential impact of a density of Polish pine wood on cutting forces with its origin region taken into consideration

Localization of ginsenosides in Panax quinquefolium root tissues