The production of virus free garlic plants from totally cvs. Jana, Mera and ecotype Zamojski was attempted by means of thermotherapy and meristem tip culture. The cloves and the aerial bulbils after hot air treatment in a growth chamber at 36°C for 30-35 days or at 26-28°C for 3-4 months in greenhouse were used to meristem tip culture on M. S. medium. In the 26-28°C treatment 19.5 % of meristems produced plants and 22.5 % of these were virus free. In the 36°C treatment 14.5 % of the meristems developed into plantlets and 34.6 % of them were virus free. The plantlets were indexed by "sap-dip" electron microscopy methods.