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1

Effect of cycloheximide on some physiological changes associated with senescence of detached flowers of Iris germanica L.

100%
Sultan S. M., Farooq S.
Acta Physiologiae Plantarum
|
1997
|
tom 19
|
nr 1
Silverthiosulphate which is a potent inhibitor of ethylene action was found to be ineffective in delaying senescence of detached flowers of Iris germanica whereas cycloheximide, a protein synthesis inhibitor, effectively delayed the senescence of these flowers and extended the longevity to 6 days. However, this treatment resulted in suppression of bud opening. When cycloheximide treatment was given at progressive intervals it became less effective in inhibiting bud opening and delaying senescence. Cycloheximide treatment maintained a higher protein content in the perianth tissue of flowers compared to untreated flowers.
2

The effect of pretreatment with colistin and cycloheximide on the albumin uptake in Tetrahymena pyriformis

86%
Szablewski L.
Acta Protozoologica
|
1989
|
tom 28
|
nr 2
3
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Content available

A moderate dose of cycloheximide does not prevent the febrile response to endotoxin but interfere with induction of pyrogenic tolerance in rabbit

86%
Kozak W., Soszynski D., Wachulec M., Szewczenko M., Bodurka M.
Journal of Physiology and Pharmacology
|
1992
|
tom 43
|
nr 2
The purpose of the present study was to examine the effect of cycloheximide (Cx) - inhibitor of protein synthesis, on the development of pyrogenic tolerance to LPS. It has been observed that Cx at a dose of 1 mg/kg given intravenously 1 h prior to LPS did not prevent fever response, however it modified the induction of pyrogenic tolerance. It was manifested in existence of the second phase of fever after the following administrations of LPS into rabbits pretreated with Cx. In control group of rabbits the induction of pyrogenic tolerance was accompanied with decaying of the second peak of fever visible as early as the second dose of LPS.
4

Mitotic activity as an early symptom of flower evocation in Pharbitis nil Chois

86%
Czaplewska J., Kopcewicz J.
Acta Physiologiae Plantarum
|
1994
|
tom 16
|
nr 1
5

The influence of cycloheximide on the induction of apoptosis in CHO cells by EDS

86%
Polak U., Warchol J. B.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
6

Regulation of NH4plus transport pathways in Pisum arvense roots

86%
Kubik-Dobosz G., Kazanecka M., Buczek J.
Acta Physiologiae Plantarum
|
1999
|
tom 21
|
nr 1
The effects of metabolic and protein synthesis inhibitors on NH₄ ⁺ uptake by Pisum arvense plants at low (0.05 mM) and high (1 mM) external ammonium concentration were studied. In short-time experiments cycloheximide decreased the ammonium uptake rate at low level of NH₄ ⁺ and increased the absorption of NH₄ ⁺ from uptake medium containing high ammonium concentration. Arsenate and azide supplied into uptake solutions at low ammonium concentration strongly decreased or completely suppressed the NH₄ ⁺ uptake rate, respectively. When the experiments were carried out at high level of ammonium only azide decreased the uptake rate of NH₄ ⁺ and arsenate stimulated this process. Dinitrophenol very strongly repressed the uptake rate of NH₄ ⁺ at both ammonium concentrations. After removing dinitrophenol from both solutions, neither at low nor high external ammonium level the recovery of NH₄ ⁺ uptake rate was achieved within 150 min or 3 h, respectively. The recovery of NH₄ ⁺ uptake rate after removing azide was observed within 90 min and 3 h at low and high ammonium concentrations, respectively. The regulation of NH₄ ⁺ uptake by some inhibitors at low external ammonium level was investigated using plasma membrane vesicles isolated from roots by two-phase partitioning. Orthovanadate completely suppressed the uptake of NH₄ ⁺ by vesicles and quinacrine decreased the NH₄ ⁺ uptake which 55 suggests that ammonium uptake depends on activities of plasma membrane-bound enzymes. On the other hand, it was found that dinitrophenol completely reduced the NH₄ ⁺ uptake by vesicles. The various effects of inhibitors on ammonium uptake dependent on external ammonium concentration suggest the action of different ammonium transport systems in Pisum arvense roots. The ammonium transport into root cells at low NH₄ ⁺ level requires energy and synthesis of protein in the cytoplasm.
7

The effect of the chemical structure of polyene macrolides on the permeability of the Chlorella vulgaris plasma membrane

72%
Malewicz B.
Oceanologia
|
1976
|
tom 06
8
Content available remote

Cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis in human colorectal cancer cell line COLO 205: role of FLIP and metabolic inhibitors

72%
Pajak B., Gajkowska B., Orzechowski A.
Journal of Physiology and Pharmacology. Supplement
|
2005
|
tom 56
|
nr 3
101-118
Current efforts are focused on revealing the cellular factors that determine the “immune escape” of cancer cells. As an example in our study human colorectal cancer cell line COLO 205 was resistant to TNF-alpha - a death inducing ligand. Nonetheless, co-incubation TNF-alpha (10 ng/ml) with cycloheximide (5 µg/ml, CHX) caused time-dependent (6, 12, 24 hours) cell death even though, at that concentration cycloheximide did not exert cytotoxic effect unless 24 hour of treatment. After additional pretreatment it is concluded that CHX sensitizes cells to TNF-alpha-induced apoptosis. The aim of this study was to investigate the role and properties of cellular proteins that granted COLO 205 cells survival as well as to assess the effect of several metabolic inhibitors on cell viability at the above-mentioned time-points. PKCs inhibitor staurosporine (5 µM) did not influence, whereas cPKC activator PMA (100 µM) prevented TNF-alpha-induced apoptosis in the presence of CHX. Although EDTA (2 mM) and to a lesser degree EGTA (5 mM) were individually cytotoxic, they exerted protective effect at 6 and partially at 12 hour of combined TNF-alpha and CHX treatment. Ionophore A23187 (1 µM) protected cells against cell death at 12 and 24 but only partially at 6 hour of treatment. On the other hand, AD (10 ng/ml) acted synergistically with TNF-alpha and CHX at 6 and 12 hour. It appears that resistance of COLO 205 cells is determined on genomic level by a few reaction steps in which both Ca2+-dependent and antiapoptotic proteins are involved. Further studies revealed that CHX treatment reduces the level of FLIP but not other members of TNF-alpha-dependent death signalosome.
9

The induction of the alteration of glutamate dehydrogenase [GDH] izoenzymes by cycloheximide in sucrose starved lupine embryos

72%
Lehmann T., Deckert J., Ratajczak L., Przybylska M.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
10
Content available remote

Position of STAT-1alpha in cycloheximide-dependent apoptosis triggered by TNF-alpha in human colorectal COLO 205 cancer cell line: role of polyphenolic compounds

58%
Pajak B., Gajkowska B., Orzechowski A.
Journal of Physiology and Pharmacology. Supplement
|
2005
|
tom 56
|
nr 3
119-141
“Immune escape” is a crucial instrument used by carcinoma cells to overcome numerous strategies of immune system to delete transformed cells. Cellular factors that make cancer cells immune to defence mechanisms are incompletely understood while some remain ambiguous. Up to date evidence points to some proteins and/or signaling molecules that might be a basis for unusual behavior of cancer cells. In particular STAT kinases are currently in the main focuse of attention since they were both shown to accelerate and/or to inhibit apoptosis. In our studies we observed that human colorectal COLO 205 cancer cells were resistant to TNF-alpha- or cycloheximide-induced cytotoxicity. However, when TNF-alpha (10 ng/ml) has been given along with cycloheximide (5 µg/ml, CHX) COLO 205 cells died extensively from apoptosis. Apparently, cycloheximide sensitized cells to TNF-alpha-induced programmed cell death. To investigate the role of STAT-1alpha in CHX-mediated TNF-alpha-induced COLO 205 cell death certain polyphenolic compounds were studied if they modulate STAT-1alpha phosphorylation status and STAT-1alpha-protein interaction at the level of TNF-alpha signalosome in the 6th, 12th, and 24th hour of experiment. Neither of phenolic compound, namely PI-3K inhibitor (LY294002, 20 µM) nor MEK inhibitor (PD98059, 50 µM), nor flavonol quercetin or kaempferol (10, 100 µM) in contrast to apigenin (20 µM) influenced COLO 205 cell viability during individual or combined treatment with TNF-alpha and CHX. We conclude, that some antiapoptotic proteins were involved but not STAT-1alpha kinase to resist TNF-alpha-dependent cell death promoting activity. Summing up, except apigenin, the above-mentioned polyphenolic componds were unable to modulate survival signal in COLO 205 cells initially believed to be suppressed by STAT-1alpha.
11

Changes in content of photosynthetic pigments and proteins in mustard (Sinapis alba, L.) cotyledons after cycloheximide and kinetin treatment

58%
Machlicova A., Slovakova L., Hudak J.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
12

Nitric oxide production by cells infiltrating amphibian skin grafts

58%
Jozkowicz A., Plytycz B.
Folia Biologica
|
1997
|
tom 45
|
nr 1-2
13

Cooling shock induction of protein synthesis by larvae of Galleria mellonella [L.] [Insecta: Lepidoptera]

58%
Sauman I., Hyrsl P., Marek M.
Biological Bulletin of Poznań
|
2001
|
tom 38
|
nr 2
14

The modifying effect of sucrose on glutamate dehydrogenase [GDH] activity in lupine embryos treated with inhibitors of RNA and protein synthesis

58%
Lehmann T., Ratajczak L., Deckert J., Przybylska M.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 4
15
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Content available

Effects of heat shock and teprenone on ethanol-induced damage to cultured rabbit gastric mucosal cells

58%
Takahashi S., Yamazaki T., Okabe S.
Journal of Physiology and Pharmacology
|
1996
|
tom 47
|
nr 3
16

Rhythm of sporulation within the section Penicillium clavigerum. III. Induction of growth and zonation rhythms in Penicillium isariiforme Stolk and Meyer 247.56

51%
Piskorz-Binczycka B.
Acta Biologica Cracoviensia. Series Botanica
|
1994
|
tom 36
In Penicilium isariiforme there occur two endogenous rhythms: the growth-“wave” rhythm and sporulation rhythm in the form of coremia-bearing zonations. The growth rhythm of wave type occurring in P. isariiforme in light and in darkness is an endogenous spontaneous rhythm, whereas the sporulation rhythm manifested by the formation of spore-bearing coremia zonations represents an analogue induced rhythm. For this rhythm to occur a light impulse is necessary. The growth-wave rhythm has a long period 120 h, the sporulation rhythm has a 30 h period. Asparagine seems to regulate the sporulation rhythm; but it does not exert any greater influence on the wave rhythm in cultures grown in light nor in darkness. An addition of asparagine shortened the period of first zonation by a few hours, and the successive sporulation periods followed within very short time. Inhibitors such as: avidin. chloramphenicol, cycloheximide and puromycine controlled the length of the period of the sporulation rhythm.
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