Subject and purpose of work: The main goal is to offer more productive approach to security culture: viewing culture as a logical system which can be understood using theories and principles developed. Materials and methods: Cultural security issue collaborative research and discovery of literature to fully exploit social networks and open source material. Results: Cultural security issue analyzes the hard and soft power of antiquities, artworks, religious monuments, and historic structures as indicators of the political economy of cultural property. Conclusions. Analyses of the political significance of historic structures and religious monuments and the licit and illicit global market for antiquities and artworks provide insights into the strategic role of cultural property in diplomacy and international security. Integrating operational cultural principles into the specific conditions is the future and will bring depth to touchstones such as “no better friend, no worse enemy,” and “first, do no harm”.
Static and stirred culture systems are widely used to expand hematopoietic cells, but differential culture performances are observed between these systems. We hypothesize that these differential culture outcomes are caused by the physiological responses of CD34+ hematopoietic stem and progenitor cells (HSPCs) to the different physical microenvironments created in these culture devices. To understand the genetic changes provoked by culture microenvironments, the gene expression profiling of CD34+ HSPCs grown in static and stirred culture systems was compared using SMART-PCR and cDNA arrays. The results revealed that 103 and 99 genes were significantly expressed in CD34+ cells from static and stirred systems, respectively. Of those, 91 have similar levels of expression, while 12 show differential transcription levels. These differentially expressed genes are mainly involved in anti-oxidation, DNA repair, apoptosis, and chemotactic activity. A quantitative molecular understanding of the influences of growth microenvironments on transcriptional events in CD34+ HSPCs should give new insights into optimizing culture strategies to produce hematopoietic cells.