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This study evaluated the effect of Pseudomonas strains and arbuscular mycorrhiza fungi (AMF) in enhancing strawberry yield and phenolic and antioxidant capacity on a phosphorus (P) deficient calcareous soil. The experiments were conducted in three replicates with six treatments (four Pseudomonas strains, AMF and control) and three rates of P-fertilizer (0, 75, 150 kg P ha–1). Application of higher phosphate rates decreased total antioxidant capacity, total phenolic and flavonols content, whereas AMF and Pseudomonas strains increased quality and P concentration of fruit. The use of AMF and Pseudomonas strains resulted in better quality when used along with 75 kg P ha–1. These results demonstrated that the rhizospheric microorganisms improved the quality of fruit, especially when they applied in combination with lower rates of chemical fertilizers. Therefore, application of these microorganisms in sustainable agriculture is recommended.
Dehydrins are proteins that play a role in the mechanism of drought tolerance. This study aimed at establishing dehydrin profile and accumulation in four local melon varieties of Iran: Mino, Dargazi, Saveii, and Semsori, as well as in a commercial variety Honeydew. Plants were treated with drought stress by adjusting the soil water content to 75, 50, 40, 30 and 20% of field capacity (FC) by withholding water. Water status of plants was monitored based on the seedling fresh weight (FW) and relative water content of leaves (RWC). Total protein content was extracted, then heat-stable protein (HSP) fraction was isolated for each variety and water stress treatment. After SDS-PAGE of HSP, Western blotting analysis was carried out with Anti-dehydrin rabbit (primary) and Goat anti rabbit (secondary) antibodies. ANOVA results showed that with decreasing FC below 75%, FW and RWC decreased, but these changes significantly varied among genotypes. On the basis of FW and RWC data under different drought stress treatments, the following drought-tolerant ranking was established: Mino > Dargazi > Saveii and Honeydew > Semsori, from tolerant to sensitive order. Results of Western blot analysis showed that expression of some proteins with molecular weights of 19– 52 kDa was induced in the studied varieties under drought stress (% FC). Expression level of the dehydrin proteins in different varieties was variable and also depending on the drought stress level applied. However, dehydrin proteins (45 and 50 kDa) showed strong expression levels in all varieties under severe drought stress (20% FC). The abundance of dehydrin proteins was higher in tolerant varieties (Mino and Dargazi) than in moderate and drought sensitive genotypes. Consequently, dehydrins represent a potential marker for selection of genotypes with enhanced drought tolerance.
W latach 1986-1988 na polu ART w Olsztynie przeprowadzono doświadczenie, w którym badano wpływ pięciu sposobów uprawy gryki uwzględniających ilość wysiewu, rozstawę rzędów, pielęgnację i nawożenie na plon i wartość siewną nasion gryki. Najwyższy plon i współczynnik rozmnażania oraz masę 1000 nasion uzyskano w kombinacji, w której z początkiem drugiej dekady maja wysiano 200 szt./m2 nasion w szerokie międzyrzędzia, stosując uprawę mechaniczną. W trzeciej dekadzie odpowiedniejszy jest wysiew 400 szt. nasion/m2 z ewentualnym dodatkowym nawożeniem i opryskiwaniem Betoksonem.
Experiments were carried out in 2006-2008 on Lysimachia vulgaris L. plants from tw natural and one cultivated populations. The following features were determined: mea number of capsules per shoot, mean number of capsules per shoot with respect to thre different levels of fruit localization in the inflorescence, seed breadth, length and germ nability. Mean number of fruits per shoot was significantly higher for cultivated plant in comparison with plants growing in natural populations. Both cultivated plants an those growing in natural habitats formed significantly more fruits in the middle part c the inflorescence in comparison with bottom and top parts of the inflorescence. Mea number of seeds per capsule depended on the fruit localization in the inflorescenc and was the highest for capsules located in the middle part of it. The seed size and gei minability after the final count depended on seed localization on a plant. The highes germinability was observed for seeds of cultivated plants produced in the middle par of the inflorescence.
Cornelian cherry is a little-known plant that can be grown in Poland both on a commercial and amateur scale. The commercial cornelian cherry plantations should be established using selected cultivars or vegetatively propagated valuable ecotypes, as only this guarantees a uniform yield, maturation and standardization of fruit quality. The experiment was carried out in 2014 and 2015 in the private plantation of cornelian cherry (Cornus mas L.) located in Dąbrowica, near Lublin (22.454 N; 51.270 E). The subject of the research were ecotypes No.: 1, 2, 3, 4, 5, 6, 7, 10, 11, 14, 15 obtained as seedlings in 1998. Cornelian cherry plants obtained from seeds differed very much in the yield and quality of fruit. The fruit characteristics for the examined ecotypes ranged to 1.63–2.21g for fruit weight, 1.30–1.61 for fruit shape index, 10.13–15.32% for content of stone, 17.85–22.68% for soluble solids content (SSC), 2.32–3.0% for titratable acidity (TA), 6.59–8.36 for SSC/TA, 54.9–75.97 for vitamin C content. Among the ecotypes studied in terms of external fruit features, ecotypes No. 3 and 4 were distinguished due to the largest fruits (mass and diameter) and relatively small share of the stone in the whole mass of the fruit. However, due to the chemical composition, the fruits of ecotype No. 5 were distinguished by the content of extract, extract to acids ratio, sugar, dry matter, anthocyanins and vitamin C content. These fruits enjoyed 55% strong acceptability among adults. The indicated ecotypes can be used in breeding as well as in nursery for obtaining valuable varieties of cornelian cherry for production in Poland.
The higher fungi are a rich source of chemical compounds with multi-directional therapeutic and pro-health effects. This review summarizes the results of the most important chemical and biological studies of the fruiting bodies and the mycelial cultures of Laetiporus sulphureus. Numerous studies have demonstrated the antimicrobial, anticancer, cytotoxic, hypoglycemic anti-inflammatory and antioxidant activity of the extracts. Currently, only a few wood-decay fungi have practical use in medicine. Therefore it seems important to continue research on the effectiveness and safety of extracts and compounds of natural origin, including fungi, whose potential is not still used.
Improved soil fertility is a requirement for enhanced crop production. Combination of inorganic and organic fertilizers improve crop productivity while reduce environmental degradation. An experiment was carried out at Eastern University, Sri Lanka to study the effect of Foliar Application of Banana Pseudostem Sap on Yield of Cowpea (Vigna unguiculata L. Walp.) in sandy regosol. The experiment was laid out in Randomized Complete Block Design having five treatments viz; recommended inorganic fertilizer N, P, K as basal and N as topdressing (T1), N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% , 3%, 5% and 7% banana pseudostem sap solution (T2-T5) at 3rd, 5th, 7th and 9th week after planting. The results revealed that significant difference (P<0.05) were noted on number of pods per plant at 1st and 2nd picking. Higher number of pods per plant, maximum pod length and girth were in T2 while low in T1. Sun dried weights of pods and number of seeds per pod were significantly (P<0.05) varied at each picking. Further, cowpea yield at each picking were higher in T2 compared to tested treatments. The present study suggested that, among the tested treatments N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% Pseudostem sap solution at 3rd, 5th, 7th and 9th WAP would be the most suitable for cowpea production in sandy regosol.
Experiments to determine the effect of seed size, watering interval and defoliation on growth and dry matter attributes of seedlings of shea butter tree were conducted at Makurdi, Nigeria, from July 2010 to April, 2012. In the first experiment, treatments were made up of three seed size categories described as small (5-10g), medium (11-15g) and large (16-20g) and three watering intervals of 3, 6 and 9 days. In the second experiment, treatments comprised three watering intervals of 3, 6 and 9 days and three levels of defoliation – 0, 50 and 100%. In both experiments, factorial combinations of the treatments were laid out in completely randomized design (CRD) and replicated three times. Results of analysis of variance showed that in the first experiment seed size significantly influenced root length with the medium and large seeds producing seedlings with comparatively longer roots than the small seeds. The most frequently watered seedlings (3 and 6 days) produced more leaves than those watered less frequently (9 days). Seed size influenced dry weight of root and total (whole plant) dry weight with the medium and large seeds recording significantly higher values than the small seeds. Watering interval however did not significantly influence any of the other traits. Seed weight exerted significant influence on amount of dry matter partitioned to the stem and the root. More dry matter was partitioned to the stem by the small seeds. Conversely, medium and large seeds allocated more dry matter to the root than to the stem. In the second experiment, defoliation level only influenced total fresh weight, dry weight of root and total dry weight in favour of plants with half their leaves removed. None of the traits examined were significantly influenced by watering regime. Interaction between defoliation level and watering regime significantly influenced total fresh weight, dry weight of root and total dry weight. Generally seed size had more influence on seedling characters than watering interval. Seedlings also demonstrated remarkable ability to overcome effects of defoliation.
Two inoculums: Effective Microorganisms (EM) specimen available on the market and microbiological BAF1 inoculum, were applied in the experiment. The plants were cultivated in the growth chamber equipped with shelves with fluorescent or LED lamps. The highest number of inflorescences was under the influence of white color of light emitted by fluorescent lamps and blue color of light emitted by LED lamps, especially after application of BAF1 inoculum. Irrespective of microbiological inoculum, no significant effect of the color of light and type of lamps on such traits as height of leaves layer, number of leaves, greening index of leaves (SPAD) and length of inflorescences, was found. The white color light emitted by fluorescent lamps stimulated actinobacteria multiplication, especially after EM application. Regardless of the inoculum application, it was the blue color light emitted by LED lamps that stimulated the multiplication of moldy fungi. After the use of fluorescent lamps, the increase in dehydrogenase activity was observed, especially after the application of BAF1 inoculum. The activity of acid phosphatase was stimulated by blue and white+blue light emitted by LED lamps. The increase in the activity of urease was observed under fluorescent lamps emitting the green, blue and white color of light, after the application of EM.
The Asian pear tree (Nashi), originated in China and Japan, where it is commonly grown. In Europe, in Poland in particular, it remains almost unknown, despite the fact that its cultivation has been proven to be possible there. The aim of the experiment – carried out between 2008 and 2015 at the Fruit Experimental Station Samotwór, next to Wrocław – was to evaluate the growth intensity and yielding of several Asian pear tree cultivars: ‘Kosui’, ‘Shinseiki’, ‘Nijisseiki’, ‘Chojuro’, ‘Hosui’, and ‘Shu Li’; as well as the quality and biological value of their fruits. ‘Conference’, which is widespread in Europe, was chosen as the control variety. ‘Conference’, ‘Shu Li’, and ‘Hosui’ showed highest growth intensity, whereas the trees of ‘Shinseiki’ were the smallest. In terms of yields over the period of eight years following the planting, ‘Chojuro’ and ‘Shinseiki’ performed best, while the least fruits were obtained from the ‘Nijisseiki’ cultivar. Significantly heaviest and biggest fruits were collected from the Chinese ‘Shu Li’ cultivar. In 2012, Nashi fruits had similar carotenoid, calcium, and magnesium contents as the fruits of ‘Conference’.
Carrot production of valuable carotenes, carbohydrate and protein are hindered by elevated salinity levels in many parts of the world. To assess this problem, germination and growth of two carrot cultivars (Daucus carota cvs Jordan and Napoli) were studied in vivo and in vitro under different salt stress concentrations (0, 75, and 150 mM NaCl). Seeds were directly or gradually exposed to these salt concentrations. With elevated salinity levels, significant reductions in growth parameters (dry shoot weight, fresh shoot weight, shoot length, root length, and root number) were observed. Also, significant difference in germination percentage was observed at 150 mM NaCl in both cultivars when compared with control treatment (90% germination percentage in Napoli and 71% in Jordan cultivar). Growth rate, tolerant index, and relative water content (RWC) declined as salinity increased. The 150 mM NaCl salinity treatment significantly reduced the shoot chlorophyll and protein content, but increased carbohydrate content. Lesser impairment by the gradual exposure of seedling to salinity provides an opportunity to study the acquirement of salt tolerance.
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