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The sterols in petrol extracts from the underground part and petioles of Rheum rhaponticum L. and Rheum undulatum L., as well as from the underground part of Rheum palmatum L. were determined by the GC-MS method. The study enabled detecting in all the samples the following sterols, as sterol acetates: cholesteryl, (3ß,24R)-ergost-5-en-3ol, (3ß,22Z)-stigmasta-5,22-dien-3ol, and ß-sitosterol. The investigated species contain the same sterols, detected in similar amounts, while ß-sitosterol is the main component of the sterol fraction of the species Rheum cultivated in Poland. Besides, vitamin E were detected in investigated species.
Wild pear (Pyrus pyraster, syn. P. communis var. pyraster) is thought to be one of the species that gave rise to all other members of the genus Pyrus, although intraspecific hybridizations with cultivated varieties could cause the disappearance of original species characteristics. S-RNase alleles from 7 different wild pear individuals, collected from various regions of Poland, were cloned on the basis of the PCR method and nucleotide sequence analyses. The hypervariable (HV) region is responsible for allele-specific S-RNase activity in the self-incompatibility mechanism. The high level of polymorphism of its sequences may constitute a source of valuable phylogenetic information. From all individuals, 14 sequences were obtained successfully, and 9 of them were novel alleles. Phylogenetic analysis of these alleles was based on the amino acid sequence interpretation of coding regions and intron nucleotide sequences. The research conducted on a limited pool of available P. pyraster alleles gives only an initial insight into possible S-RNase allele polymorphisms in wild populations. At this stage, the results do not confirm a strong influence of cultivated pear species on the wild pear.
Beta genus belonging to Chenopodiaceae family consists of four very morphologically and genetically differentiated sections: Beta, Corollinae, Procumbentes and Nanae. Wild Beta species are important as a source of genes resistant to diseases, pests and abiotic factors. The paper presents collecting, documenting, evaluating and preserving genetic resources of the Beta genus. At present, in the gene bank of the National Centre for Plant Genetic Resources at Radzików there are 530 accessions: 112 sugar beet, 386 fodder beet and 32 accessions of wild beet. Accessions have been obtained from national breeding institutions mainly and in the way of exchange among beet collections and foreign research laboratories. The material received from international expeditions (local populations) is also of great interest. The collection is being increased by 10-30 accessions every year. All collected materials were evaluated and statistical data analyzed and documented according to the international standards for Beta genus. Utilization of the collection materials is determined by the main trends in breeding and research. In the last years some of the accessions were evaluated for two economically important beet diseases: Aphanomyces cochlioides DRECHSLER and Cercospora beticola Sacc. At present, an attempt is made to select some accessions tolerant to drought stress. Also fast progress in tissue culture technique and genetic engineering gave a new chance for germplasm utilization. On the basis of collected materials five fodder beet cultivars were obtained (Magdalena, Juta, Jarko, Jaspis and Jawor, cvs).
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