Wyniki wyszukiwania

1

Changes in creatine kinase and lactate dehydrogenase activity, and in myoglobin concentration during strength efforts

100%

Slowinska M., Stefaniak T., Sobiech K. A.

Polish Journal of Environmental Studies

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1998

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tom 07

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nr 6

2

The transcriptional cascade associated with creatine kinase down-regulation and mitochondrial biogenesis in mice sarcoma

86%

Bera S., Ray M.

Cellular and Molecular Biology Letters

|

2009

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tom 14

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nr 3

481-496

The tissue-specific expressions of creatine kinase (CK) isoforms are regulated by the coordinated action of various transcription factors. The myogenic differentiation factor D (MyoD) family of proteins and the myocyte-specific enhancer binding factor 2 family of transcription factors are important in regulating the muscle-specific expression of cytosolic muscle-type CK (MCK) and mitochondrial CKs. As reported in some related studies, TNF-α mediated degradation of MyoD and myogenin mRNA may lead to severe muscle wasting and cachexia, which is characterized by a low transcript level of MCK and myosin heavy chain proteins. In our previous study, we reported on a complete loss of total CK activity and expression when sarcoma was induced in mouse skeletal muscle (Patra et al. FEBS J. 275 (2008) 3236–3247). This study aimed at investigating the transcriptional cascade of CK down-regulation in carcinogen-induced sarcoma in mouse muscle. Both CK deficiency and enhanced nitric oxide synthase (NOS) were known to augment mitochondrial biogenesis, so we also explored the activation of the transcriptional cascade of mitochondrial biogenesis in this cancer. We observed the activation of the TNF-α-mediated nitric oxide production pathway with NFκB activation and concomitant degradation of MyoD and myogenin mRNA. Exploration of mitochondrial biogenesis revealed high cytochrome c oxidase activity and mitochondrial DNA content in sarcoma. The PGC-related co-activator seems to have a major role in regulating mitochondrial biogenesis by upregulating nuclear respiratory factors and mitochondrial transcription factor A. From the above findings, it can be concluded that severe muscle degeneration leads to CK down-regulation in sarcoma, and that the stimulation of mitochondrial biogenesis indicated a scenario representing both CK deficiency and NOS overexpression on the one hand, and altered bioenergetic profiling on the other.

3

Lactate concentration and creatine kinase activity after 110-m and 400-m hurdles races

86%

Krysciak J., Podgorski T., Eichler A.

Trends in Sport Sciences

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2015

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tom 22

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nr 4

Introduction. 110-m and 400-m hurdles races are based on anaerobic energy metabolism which can induce muscle fatigue and muscle fiber damage. The most common biochemical parameters used in controlling athletes’ training loads and post-exercise fatigue are blood lactate (La) concentration and creatine kinase (CK) activity. Aim of Study. The aim of the study was to determine and compare runners’ biochemical response after 110-m and 400-m hurdles races. The influence of warm-up before both races was taken into consideration. Material and Methods. Eight male hurdlers took part in this research. They were subjected to two test exercises: a 110-m and a 400-m hurdles race. During each test, pre-warm-up, post-warm-up, and post-exercise capillary blood from a fingertip was collected to determine the La concentration and CK activity. Furthermore, during both sprint runs the athletes’ time (to the nearest 0.01 s) and heart rate (HR) were measured. Results. Each kind of exercise test increased the La concentration and CK activity. More significant changes of both biochemical parameters occurred after the 400-m race. Furthermore, after each warm-up significant increases of La and CK levels were observed. After the 400-m hurdles race higher HR values were noted (184.50 ± 8.32 compared to 177.50 ± 11.14 after the 110-m sprint). Conclusions. Both specialist warm-up and 110 and 400 meters hurdles races lead to significant changes in athletes’ physiological and biochemical blood parameters. La concentration and CK activity demonstrate greater muscle fatigue and muscle fiber damage after a 400-m than a 110-m hurdles race.

4

A spectrophotometric assay of tributyltin-induced creatine kinase release from herring spermatozoa

86%

Grzyb K., Rychlowski M., Skorkowski E. F.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

5

Gender dimorphism in the exercise-naive murine skeletal muscle proteome

72%

Metskas L. A., Kulp M., Scordilis S. P.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 3

507-516

Skeletal muscle is a plastic tissue with known gender dimorphism, especially at the metabolic level. A proteomic comparison of male and female murine biceps brachii was undertaken, resolving an average of 600 protein spots of MW 15–150 kDa and pI 5–8. Twenty-six unique full-length proteins spanning 11 KOG groups demonstrated statistically significant (p<0.05) abundance differences between genders; the majority of these proteins have metabolic functions. Identified glycolytic enzymes demonstrated decreased abundance in females, while abundance differences in identified oxidative phosphorylation enzymes were specific to the proteins rather than to the functional group as a whole. Certain cytoskeletal and stress proteins showed specific expression differences, and all three phosphorylation states of creatine kinase showed significant decreased abundance in females. Expression differences were significant but many were subtle (≤ 2-fold), and known hormonally-regulated proteins were not identified. We conclude that while gender dimorphism is present in non-exercised murine skeletal muscle, the proteome comparison of male and female biceps brachii in exercise-naive mice indicates subtle differences rather than a large or obviously hormonal dimorphism.

6

Oxidative stress in standardbred horses during official races of 1600 and 2000 meters

72%

Piccione G., Fazio F., Giudice E.

Medycyna Weterynaryjna

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2007

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tom 63

|

nr 12

1554-1557

In thoroughbred race horses, as in other species subjected to physical activity, there is a rise in the imbalance between free radical production and antioxidant agents which leads to oxidative stress. This stress may produce damage in several bio-molecules creating metabolic alterations affecting physical performance. The aim of this study was to find possible relationships between physical exercise and oxidative stress in trained horses. In order to achieve this we reported the results obtained while studying the effect of a physical exercise test on two groups of standardbreds. In particular, the study assessed levels of creatine kinase (CK) and aspartate amino transferase (AST) to evaluate possible muscle-cell membrane damage; reactive oxygen species (ROS), thiol antioxidant barrier (SHp) and antioxidant barrier (Oxy-adsorbent) to evaluate oxidative stress. Two groups of healthy standard bred (Ga and Gb) trained for 1600 and 2000 meter races were used for the study. Blood samples from all horses were collected at rest, immediately after racing, and 30 and 6 hours after racing. The ANOVA for repeated measures showed the highly significant effects of training on some of the studied parameters in both groups (Ga and Gb). Our results seem to indicate that in trained standard breeds acute exercise generates free radicals but they are unable to cause possible muscle-cell membrane damage. However, in order to know whether the inhibition of oxidative processes during exercise benefits physical performance, it would be necessary to simultaneously measure parameters relating to work capacity such as blood lactate, heart rate and oxygen consumption, both in basal conditions and at different times after a standardized race.

7

The function of complexes between the outer mitochondrial membrane pore [VDAC] and the adenine nucleotide translocase in regulation of energy metabolism and apoptosis

72%

Vyssokikh M. Y., Brdiczka D.

Acta Biochimica Polonica

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2003

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tom 50

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nr 2

The outer mitochondrial membrane pore (VDAC) changes its structure either volt- age-dependently in artificial membranes or physiologically by interaction with the adenine nucleotide translocase (ANT) in the c-conformation. This interaction creates contact sites and leads in addition to a specific organisation of cytochrome c in the VDAC-ANT complexes. The VDAC structure that is specific for contact sites generates a signal at the surface for several proteins in the cytosol to bind with high capacity, such as hexokinase, glycerol kinase and Bax. If the VDAC binding site is not occupied by hexokinase, the VDAC-ANT complex has two critical qualities: firstly, Bax gets access to cytochrome c and secondly the ANT is set in its c-conformation that easily changes conformation into an unspecific channel (uniporter) causing permeability transition. Activity of bound hexokinase protects against both, it hinders Bax binding and employs the ANT as anti-porter. The octamer of mitochondrial creatine kinase binds to VDAC from the inner surface of the outer membrane. This firstly restrains interaction between VDAC and ANT and secondly changes the VDAC structure into low affinity for hexokinase and Bax. Cytochrome c in the creatine kinase complex will be differently organised, not accessible to Bax and the ANT is run as anti-porter by the active creatine kinase octamer. However, when, for example, free radicals cause dissociation of the octamer, VDAC interacts with the ANT with the same results as described above: Bax-dependent cytochrome c release and risk of permeability transition pore opening.

8

Genes and physical fitness

58%

Gronek P., Holdys J.

Trends in Sport Sciences

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2013

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tom 20

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nr 1

The search for genes with that positively affect physical fitness is a difficult process. Physical fitness is determined by numerous genes, and its genetic determinants are modified by environmental factors. The map of candidate genes that can potentially affect physical fitness becomes larger every year, and currently it contains more than 200 genes associated with such aspects as respiratory and cardiovascular stability; body build and composition – especially muscle mass and strength; carbohydrate and lipid metabolism; response to training; and exercise intolerance. The inclusion of the genetic component in physiological and biochemical studies would permit drawing a representation of predispositions for each athlete interested in practicing high performance sports and would be a valuable coaching aid in the process of training individualization.

9

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Slaughter value, meat quality, creatine kinase activity and cortisol levels in the blood serum of growing-finishing pigs slaughtered immediately after transport and after a rest period

58%

Smiecinska K., Denaburski J., Sobotka W.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

The experimental materials comprised 44 hybrid [ (♀Polish Large White x Polish Landrace) x ♂Duroc] growing-finishing pigs. The animals were randomly divided into two groups: 24 pigs were slaughtered immediately after transport and 20 pigs were slaughtered after a 24-hour rest period in the lairage. The meat content of pork carcasses, carcass dressing percentage, the proximate chemical composition, physicochemical and sensory properties of meat and shear force values were determined. Serum creatine kinase activity and cortisol levels were determined in blood samples collected before transport and during carcass bleeding. Pigs slaughtered immediately after transport, compared with those slaughtered after a 24-hour rest period, were characterized by a higher meat content of the carcass and a higher carcass dressing percentage. Pre-slaughter handling had no effect on pork quality. The incidence of normal-quality meat, partially PSE (pale, soft, exudative) meat and PSE meat was similar in both groups. Chemical analysis showed that the content of dry matter, total protein, fat and minerals in meat was comparable in both groups. As regards the functional properties of the pork, samples from the carcasses of pigs that had rested before slaughter had a higher contribution of the red color component. Meat from pigs slaughtered immediately after transport had more desirable sensory properties. Pre-slaughter resting had a significant effect on those analyzed physiological parameters which were found to be good indicators of pre-slaughter stress. Serum creatine kinase activity and cortisol levels were higher in blood samples collected after transport (during carcass bleeding) than in samples collected before transport, pointing to a strong stress response of animals to pre-slaughter treatment. The decrease in serum cortisol levels in blood samples collected during bleeding from the carcasses of pigs slaughtered after a 24-hour rest period, compared with samples collected from animals slaughtered immediately after transport, suggests that rest before slaughter alleviated stress induced by pre-slaughter handling operations.

10

Hawthorn revisited: time- and dose-dependent cardioprotective action of WS-1442 special extract in the reperfusion-induced arrhythmia model in rats in vivo

58%

Zorniak M., Porc M. P., Krzeminski T. F.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 2

11

Reperfusion after brief repetitive ischemia in porcine myocardium does not alter expression of creatine kinase MM or mitochondrial ATPase mRNAs1

58%

Andres J., Sharma H. S., Sassen L. M. A., Duncker D. J., Verdouw P. D., Schaper W.

Journal of Physiology and Pharmacology

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1993

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tom 44

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nr 4

12

Influence of sodium selenite and emulsigen injection on the concentration of mineral elements and activity of creatine kinase and asparagine and alanine transaminases in sheep serum

58%

Bik D.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 2

The experiment was performed on 24 ewes divided into 4 groups. Experimental sheep were injected parenterally either with a sodium selenite and Emulsigen mixture or sodium selenite or Emulsigen. The injection of sodium selenite and Emusigen mixture, and Emulsigen alone substantially increased serum copper and magnesium concentrations. Moreover, a marked decrease in creatine kinase activity was found in all experimental groups as compared to that in the controls. No significant alterations in iron, zinc, calcium, and phosphorus concentrations and the enzyme's activities were noted.

13

Acute lead toxicity and energy metabolism in rat brain synaptosomes

58%

Struzynska L., Dabrowska-Bouta B., Rafalowska U.

Acta Neurobiologiae Experimentalis

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1997

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tom 57

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nr 4

14

Levels of selected biochemical indicators of serum and blood during subclinical forms of nutritional muscular dystrophy in lambs

58%

Sobiech P., Kuleta Z.

Polish Journal of Veterinary Sciences

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1999

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tom 02

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nr 1

Selenium level, and Ihe activity of GSH-Px, AST, CK, LDH and its isoenzymes were estimated in 30 lambs suspected of having the subclinical form of nutritional muscular dystrophy (NMD) and in 30 healthy lambs. A significant Se deficiency, a reduction in the activity of peroxidase glutathione, an increase in creatine kinase, aspartate aminotransferase and lactate dehydrogenase activities were found in the unhealthy lambs. Isoenzymatic profile of LDH showed 5 tractions. Tń the unhealthy lambs, there was a significant increase in LDH5 fraction-muscular isoenzyme, which is specific to the skeletal muscle. These indicators however, remained within a normal physiological range in the healthy lambs.

Ograniczanie wyników

Changes in creatine kinase and lactate dehydrogenase activity, and in myoglobin concentration during strength efforts

The transcriptional cascade associated with creatine kinase down-regulation and mitochondrial biogenesis in mice sarcoma

Lactate concentration and creatine kinase activity after 110-m and 400-m hurdles races

A spectrophotometric assay of tributyltin-induced creatine kinase release from herring spermatozoa

Gender dimorphism in the exercise-naive murine skeletal muscle proteome

Oxidative stress in standardbred horses during official races of 1600 and 2000 meters

The function of complexes between the outer mitochondrial membrane pore [VDAC] and the adenine nucleotide translocase in regulation of energy metabolism and apoptosis

Genes and physical fitness

Slaughter value, meat quality, creatine kinase activity and cortisol levels in the blood serum of growing-finishing pigs slaughtered immediately after transport and after a rest period

Hawthorn revisited: time- and dose-dependent cardioprotective action of WS-1442 special extract in the reperfusion-induced arrhythmia model in rats in vivo

Reperfusion after brief repetitive ischemia in porcine myocardium does not alter expression of creatine kinase MM or mitochondrial ATPase mRNAs1

Influence of sodium selenite and emulsigen injection on the concentration of mineral elements and activity of creatine kinase and asparagine and alanine transaminases in sheep serum

Acute lead toxicity and energy metabolism in rat brain synaptosomes

Levels of selected biochemical indicators of serum and blood during subclinical forms of nutritional muscular dystrophy in lambs