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Gromysz H., Karczewski W. A., Jernajczyk U.: Motor nucleus of the V-th nerve and the control of breathing. Brener-Hering reflexes and apneustic breathing. Acta Physiol. Pol. Earlier studies from this department have demonstrated that neurones of the V-th nerve motor nucleus (NVmt) have oligosynaptic, inhibitory output to the inspiratory motoneurone themselves under the influence of a polysynaptic input from vagal afferents. To check the hypothesis that NVmt is a part of the pneumotaxic mechanism, we studied the effects of pharmacological microblockade of the NVmt on Breuer-Hering reflexes in halothane-anesthetized, paralyzed and artificially ventilated rabbits. Activities of NVmt neurones and phrenic nerve firing were recorded. Acid-base balance was controlled and histologic examinations were routinely performed. Expiratory activities were regulatory found in NVmt. Its blockade elicited a typical apneustic breathing. During the blockade the Breuer-Hering reflexes gave “paradoxical” effects: an increase in central respiratory frequency following inflation, inspiratory apneusis in response to deflation. We conclude that the NVmt is an important component of the pneumotaxic mechanism or even the anatomical substrate of the pneumotaxic “centre”.
Vestibular ganglion was investigated in human embryos during developmental stages l3-23 (34-56 postovulatory days). Branches of the vestibular ganglion forming ampullar nerves were observed in ernbryos at stage I5. During the last embryonic stages (stage 22 and 23) the utriculo-ampullar, posterior ampullar, and saccular nerves were clearly distinguished.
Investigations were made on serial sections of human embryos at developmental stages from 13 to 23 (32-56 postovulatory days). The trochlear nucleus appears in the posterior region of the basal plate of the midbrain at stage l3. It is composed of large neurons, the processes of which are sharply defined. Since stage l5 the trochlear fibers can be followed through the alar region to their decussation in the mid-dorsal part of the midbrain. The trochlear nerve emerges from the dorsal surface of the lowest part of the midbrain.
The aim of our study was to describe anatomical variability of the root entry zone (REZ), also called the Ohersteiner--Redlich zone, that represents the "junction zone" of glia and Schwann sheath of the cranial nerves. This zone has some clinical implications. The pulsatile compression of REZ by a vessel may produce clinical symptoms, such us trigeminal neuralgia, hemifacial spasm, glossopharyngeal neuralgia torticollis spasmodicus or even symptoms of essential hypertension when a vascular crross compression of REZ of a left vagus nerve is present. The vessel - cranial nerve contact in the skull base cysterns may be visualized in radiologic examinations, most accurately in magnetic resonance imaging. Because, we cannot distinguish the REZ from the rest of the vagus nerve in radiologic examinations we decided to measure the lenght of its REZ. The microanatomical study of the lenght of REZ zone of the vagus nerve was performed on 2l nerves taken from 17 human brain stems (12 men, 5 women, 14 left, 7 right), fixed with 8% buffered, formalin solution. Paraffin embedded tissue was cut into 1O-um-thick sections pararellel to the nerve longitudinal axis and stained with hematoxilin & eosin. Each of the nerves showed the presence of a zone of oligodendrocyte myelination, mean lenght 2 ą 0.3 mm. In 17 nerves the transitional zone formred a cone-like process, in 4 nerves was shaped irregularly. The length of REZ (oligodendrocyte myelination plus "glial dome") had the mean length 3.5 ą 0,9 mm.
The cochlear ganglion was investigated in serially sectioned human embryos at developmental stages 18 and 19 (44-46 postovulatory days). During this period the cocchlear ganglion is separated from the vestibular ganglion, and it is adjacent to the cochlear duct. In embryos of investigated stages fibers of the cochlear ganglion enter the nuclei in the brain.
The study was conducted on 6 human embryos at stage 13. It was found that the facial-vestibulocochlear complex is closely related to the otic vesicle, and the particular components of that complex may be distinguished. They show different cellular arrangement and shape. The neural crest cells migrating from the dorsal hindbrain are continuous with cells forming the cochlear ganglion. This gives evidence for neural crest contribution to the cochlear ganglion.
Variations involving the cervical portion of the vagus nerve are seemingly very rare. We report an adult male found to harbour a right cervical vagus nerve that crossed anterior to the right common carotid artery to terminate in the lateral aspect of the thyroid gland. A very small continuation of this nerve was found to continue distally into the thorax. Histologically, this part of the vagus nerve did not contain ganglion or other cell bodies. There were no heterologous inclusions (thyroid, parathyroid, thymus, salivary gland or branchial cleft remnants) present. Although grossly there was a connection into the thyroid gland, this was not observed histologically. No signs of trauma were found to the ipsilateral neck region. We hypothesise that this variation is due to entanglement between the thyroid gland and cervical vagus nerve during development. This rare variation might be considered by the clinician who operates in the cervical region or interprets imaging of the neck. To our knowledge, a vagus nerve with the above described morphology has not been described.
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