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The objective of the study was to investigate the effect of an energy restriction on serum cortisol and its faecal metabolite (11,17-dioxoandrostan) in Chios ewes in late gestation. Twenty pregnant and ten non-pregnant out of 38 randomly selected ewes were detected with ultrasound and were separated into three groups (n=10) on day 105 after mating: normal energy fed pregnant group (NE), restricted energy fed pregnant group (RE), and non-pregnant group (NP). Blood samples were taken on days 117, 130, and 144 of pregnancy and faecal samples were taken twice a week starting on day 106 of pregnancy from both pregnant and non-pregnant ewes. After extraction of serum and faecal samples, concentrations of cortisol and 11,17-dioxoandrostan (11,17- DOA) were measured. A significant decrease in RE was determined only on day 144 of pregnancy (P≤0.05). Between the two pregnant groups (NE and RE), a significant difference was found only on day 144; at this period, RE had lower serum concentrations. RE had significantly lower 11,17-DOA concentrations in late pregnancy than NE. In pregnant ewes, the levels decreased in a late gestational period. The present data shows that in ewes fed 14.47% crude protein and 8.82 MJ /kg of metabolic energy in late pregnancy, pregnancy toxaemia does not occur. We suggest that the determination of Cortisol metabolites in faeces is more objective and safe than that of serum Cortisol concentrations.
Background. Ectoparasitic infections of fish are considered to be stress inducing. However, only a few studies have addressed this topic. In addition, formalin bath treatment of rainbow trout is a generally applied method of controlling ectoparasites, but the stress response in rainbow trout, Oncorhynchus mykiss, as a result of such treatments with formalin, has not received much attention and therefore needs further elucidation. The present investigation addresses the stress-response induced by parasites and formalin treatment. Materials and Methods. Concentrations of plasma cortisol were monitored using ELISA. Samples were taken from groups subjected to confinement stress, infection with Ichthyophthirius multifiliis and formalin bath treatment. Results. Rainbow trout clearly responded to harmful stimuli by increasing plasma cortisol concentrations. Confinement, formalin bath treatment, as well as infection with Ichthyophthirius multifiliis resulted in significant high cortisol concentrations in plasma compared to the unhandled and uninfected control groups. Conclusion. The present study showed that cortisol release in rainbow trout is associated with infection with the skin ciliate Ichthyophthirius multifiliis. Formalin, which is used to control the parasite infection, also elicited a high production of this immuno-suppressing hormone in the host.
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Plasma adrenomedullin response to maximal exercise in healthy subjects

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The aim of the study was to find out whether maximal exercise performed by healthy young men influences plasma adrenomedullin concentration (ADM) and is the peptide level related to the cardiovascular, metabolic and hormonal changes induced by exercise. Ten subjects (age 24 ± 1.0 yr) participated in the study. They performed graded bicycle ergometer exercise until exhaustion. Heart rate (HR) and blood pressure (BP) were measured throughout the test. Before and at the end of exercise venous blood samples were taken for [ADM], noradrenaline [NA], adrenaline [A], growth hormone [hGH], cortisol and lactate [LA] determination. Plasma [ADM] decreased during exercise from 1.71 ± 0.09 to 1.53 ± 0.10 pmol × l-1 (p< 0.01). This was accompanied by increases in plasma catecholamines and [hGH], while plasma cortisol level did not change. Positive correlation was found between the exercise-induced decreases in plasma ADM and diastolic BP. Blood [LA], systolic and mean BP at the end of exercise correlated negatively with plasma [ADM]. No significant interrelationships were found between plasma ADM, catecholamines or the other hormones measured. The present data suggests, that maximal exercise inhibits ADM secretion in young healthy men. Metabolic acidosis and a decrease in peripheral resistance might be involved in this effect.
We tested whether simulation of postural changes, which occur during public speech test procedures, activates cardiovascular system and stress hormone release that could interfere with the effect of psychosocial stress load. Young healthy male volunteers (n=8) underwent procedure imitating exactly all postural changes present in the psychosocial stress model based on public speech used in this laboratory (namely changes from sitting to standing and repeated sitting). Postural changes were associated with increases in heart rate, blood pressure, plasma concentrations of noradrenaline and aldosterone and elevation in plasma renin activity. In contrast to cardiovascular parameters, adrenocorticotropic hormone, cortisol and adrenaline, the main characteristics of hormonal response during mental stress, were not significantly influenced. The overall magnitude of all observed alterations was much smaller than that seen following mental stress procedures in our previous studies. This study provides evidence that changes in body posture during public speech test procedure influence hemodynamics and endocrine responses in a mild manner. Though this influence may represent a source of unspecific variance, substantial confounding effects on responses to the psychosocial component of the procedure are unlikely. In any case, models combining mental stressors and changes in body posture must be interpreted as complex stress stimuli.
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Twelve male, sedentary volunteers (22.0 ± 0.7 yrs) were submitted to three weeks of a bicycle ergometer training, consisting of 45 min exercise (at 70% VO2max), 4 times in the first week and 3 times in the next 2 weeks. They performed four incremental exercise tests with the power output increased by 50 W every 3 min until volitional exhaustion: two before training (C1 and C2), and after one (T1) and three (T3) weeks of training. Before and after each load the plasma noradrenaline (NA), adrenaline (A) and blood lactate (LA) concentrations were determined in venous blood samples as well as plasma growth hormone (HGH) and cortisol concentrations before and at the end of exercise. A decrease in NA concentration was found already after 1 week of training at power output of 100 W (p<0.01) and 200 W (p<0.05). Similar decline was maintained after 3 weeks of training. No significant training-induced differences in plasma A concentration were found, however, the thresholds for both catecholamines were significantly shifted towards higher values after 3 weeks of training. One week of training caused a decrease in the pre-exercise (p<0.01), as well as post-exercise (p<0.05) plasma cortisol and HGH concentrations. It was concluded that endurance training induced a decrease in HGH, cortisol and NA concentration already after one week of training. A decline of pre-exercise plasma HGH and cortisol levels with time of experiment may, in part, indicate familiarization to exercise protocol.
Four cows were treated on day 260 of pregnancy with 300 mg prednisolone succinate i.m., and four other cows with saline solution. Blood samples were taken 4 times daily during a period of 5 days. The concentrations of cortisol progesterone and estrone sulphate in plasma were determined using radioimmunoassay. After V administration of prednisolone the level of Cortisol was depressed for 3 days and returned to initial values afterwards. The level of progesterone was lowered during this time, but the decrease was not statistically siginificant. The estrone sulphate level was not significantly altered by prednisolone administration. In the control cows receiving saline, the concentration of hormones remained nearly constant. There was no statistical difference in gestation length compared to the control group. The results obtained indicate that the suppression of adrenal cortex function in cows on day 260 of pregnancy had little or no effects on progesterone and estrone sulphate. Thus, it appeared that the adrenal contribution to the peripheral blood plasma level of progesterone is small and the synthesis of estrone sulphate by bovine placenta is probably independent of maternal adrenal precursors.
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