Wyniki wyszukiwania

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Structural and serological characterization of Hafnia alvei lipopolysaccharide core region

100%

Lugowski C., Niedziela T., Jachymek W., Klonowska A., Czarny A., Rowinski S., Petersson C., Kenne L.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

The structures and serological activities of core oligosaccharide of Hafnia alvei strains have been investigated. Methylation analysis, NMR spectroscopy and various specific degradation procedures were the principal methods used. It is concluded that, core hexasaccharides are identical in the lipopolysaccharides tested and are built of two glucose, three heptose and one 2-keto-3-deoxyoctulosonic acid residues. The antiserum raised against the ATCC13337oligosaccharide core-tetanus toxoid conjugate cross-reacted strongly with all lipopolysaccharides used as antigens in ELISA test, suggesting that this core region is the common structure in the Hafnia genus.

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Serological characterization of the core region of lipopolysaccharides of rough Proteus sp. strains

84%

Palusiak A., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

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2009

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tom 57

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nr 4

303-310

3

Application of two different kinds of sera against the Proteus penneri lipopolysaccharide core region in search of epitopes determining cross-reactions with antibodies

84%

Palusiak A., Dzieciatkowska M., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

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2008

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tom 56

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nr 2

4

Application of the potential model to the analysis of regional differences in Poland

84%

Czyz T.

Geographia Polonica

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2002

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tom 75

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nr 1

5

Polish core and periphery under economic transformation

84%

Rykiel Z.

Geographia Polonica

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1995

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tom 66

6

Transformation of Poland’s spatial-economic structure in the years 1998–2008

67%

Czyz T.

Quaestiones Geographicae

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2012

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tom 31

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nr 2

7

Comparison of serological specificity of anti-endotoxin sera directed against whole bacterial cells and core oligosaccharide of Escherichia coli J5-tetanus toxoid conjugate

67%

Lukasiewicz J., Jachymek W., Niedziela T., Malik-Gebicka M., Dzieciatkowska M., Lugowski C.

Acta Biochimica Polonica

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2002

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tom 49

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nr 3

The rough mutants of Gram-negative bacteria are widely used to induce protective antisera but the nature of the target epitope for such antibodies is not precisely defined. Endotoxin is one of several antigens present on the surface of bacterial cells, which are able to elicit specific antibodies. We studied the specificity of antibodies produced against a conjugate of E. coli J5 endotoxin core oligosaccharide with tetanus toxoid. The use of chemically defined antigen for immunisation excludes the possibility of production of antibodies against other cell surface antigens. A comparison of this monospecific anti-endotoxin serum with antiserum against E. coli J5 whole cells was performed in order to distinguish the role that endotoxin core oligosaccharide plays in the interaction with humoral host defences from that of other potentially important Gram-negative bacterial surface antigens. The reactivity of both sera with smooth and rough lipopolysaccharides was determined in ELISA, immunoblotting and by flow cytometry. Both antisera reacted with similar specificity with most lipopolysaccharides of identical or related core type. Less distinct reactions with endotoxins of the antibacterial serum in comparison with the anti-conjugate serum were found in all serological tests. LPS of E. coli O100 that showed the strongest reactions with both sera was used to stimulate IL-6, TNFα and nitric oxide production by the J-774A.1 cell line. Both sera were used to inhibit that stimulation and no inhibitory effects of the examined sera in comparison with non-immune serum were observed.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Archivum Immunologiae et Therapiae Experimentalis

2 Geographia Polonica

1 Quaestiones Geographicae

2 Czyz T.

2 Dzieciatkowska M.

2 Jachymek W.

2 Lugowski C.

2 Niedziela T.

2 Palusiak A.

2 Sidorczyk Z.

1 Czarny A.

1 Kenne L.

1 Klonowska A.

1 Lukasiewicz J.

1 Malik-Gebicka M.

1 Petersson C.

1 Rowinski S.

1 Rykiel Z.

1 2012

1 2009

1 2008

2 2002