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The study was aimed at determining the content of copper in milk fat. Analyses were carried out on fat extracted from non-pasteurised sweet cream obtained from bulk milk. Copper was extracted from fat samples with nitric acid and its content was determined with the method of flameless atomic absorption spectrophotometry. The results obtained indicate that the content of copper in milk fat ranged from 0.010 to 0.089 mg/kg. The average copper content of fat from the summer feeding accounted for 0.037±0.020 mg/kg, and that of fat from the winter feeding was significantly higher (α=0.05) and reached 0.054±0.021 mg/kg.
This paper presents the results of studies of the content of copper in the 0-20 cm layer of farmland soils in Poland and the correlation between the content of this element in the soil and the content of the granulometric fractions < 0.02 and the < 0.002, organic matter and pHKCl. The Cu content determined in soils varies between 0.2 and 725.0 whilst the expected Cu content is in the range 3.1-13.6 mg kg-1. The geometrical and arithmetical mean values of the copper content in soils amount to 6.5 and 8.6 mg kg-1 respectively. Approximately 97% of the countrys agricultural soils have a natural Cu content (0o - unpolluted soils) whereas 3% have a slightly elevated content of Cu (Io). Soils with different degrees of Cu pollution (weakly polluted -IIo, medium heavily polluted - IIIo, heavily polluted - IVo, very heavily polluted - Vo) represent only 0.40% of farmlands area. Soils in industrialised regions contain higher quantities of copper and are polluted with Cu to a greater degree than are the rural soils nationally.
The Cu and Ni contents in aboveground biomass of oats (Avena sativa L.) used for haylage making were studied in the two-year pot experiment, which included five variants: control (not fertilised), NPK, farmyard manure, compost from Krakow and compost from Červený Újezd (Tab. 2 and 3). The final nutrients dose was IS g N, 1,1 g P and 1.75 g K-pot-1. Fertilisers were applied at one before sowing in 2001, and in the second year only 0.75 g N was added to all pots as ammonium nitrate. In 2001 the highest yield of aboveground oats biomass was obtained after mineral NPK fertilisation (Fig. 1). A significantly higher yield was noted also when compost from Červený Újezd was applied. In 2002, the biomass yields in all fertilised variants were uniform and significantly higher than in the control, and the highest yield was obtained after compost from Krakow application. In 2001, the highest Cu and Ni content in the aboveground oats biomass were found after mineral NPK application (Fig. 2 and 3). In the second year, Cu content was similar in all dressed variants and significantly higher than in control plants, and the Ni content was almost identical. The both elements contents did not exceed limits acceptable for dry feeds.
The content of trace elements (Cu, Cr, Ni, Pb, and Zn) in hair and skin of silver fox (Vulpes vulpes) and arctic fox (Alopex lagopus) were determined. The content of elements were determined using inductively coupled plasma atomic emission spectrometry (ICP-OES). Samples of animal hair and skin were collected from two farms located in mid-western Poland: farm A, situated in a rural area and farm B, situated in a typically urbanized and industrial region. The influence of farm location on the concentration of Cr, Cu, and Zn in fur coat, and Cr and Ni in skin was observed.
Progressing urbanization results in the potential increase of pollution sources such as wastes, industrial or municipal sewage, which may contain hazardous inorganic pollutants such as e.g. metal ions and their compounds. Urbanization has a negative effect also on soil, as a consequence of an increasing share of impermeable surfaces such as pavements, parking lots, housing developments and public buildings. The area of a hardened (impermeable) surface is exposed to intensive surface runoff during rain and as a result pollutants are transported through the storm drainage system directly to surface waters. The aim of this study was to determine the effect of metals contained in rainwater discharged from subcatchments of various land use types on the quality of surface waters (the Cybina River and the Antoninek reservoir) in the city of Poznań, as well as investigate whether metal contents in rainwater effluents depend on the relief and character of the immediate catchment. Investigations were conducted in 2009 along the lower section of the Cybina River. Water samples from the river and reservoir were collected each time when collecting rain water samples. Water and sewage samples were collected 15 times within a year. Analyses were conducted on 5 selected catchments (with different land uses) drained by storm water drainage system discharged to the Cybina River and the Antoninek reservoir. Water was collected from 13 sampling points (5 of which were sewer outfalls and 8 were situated on the river or the reservoir above and below the sewage discharge). Higher concentrations of all tested elements were recorded in rainwater in comparison to the water samples coming from the river or the reservoir. Canonical Variate Analysis (CVA) model presenting differences between water concentrations of Cu, Zn, Cd, Pb and Fe and environmental variables (rainfall intensity measured in a four-point scale, location as divided into industrial areas multi- and single-family housing as well as location of sewage discharge to the river on the reservoir) showed elevated concentrations of these elements especially in water collected directly from the storm water sewer. Elevated metal concentrations in storm system effluents did not have a significant effect on the content of these pollutants in the river or the reservoir. It could have been caused by the intensive immobilization of elemental ions in bottom deposits followed by the metal uptake by aquatic organisms, mainly plants.
The aim of the investigation was to confirm the hypothesis put forward in human research that copper is cumulated in the neoplastic tumours of the mammary gland. The research material included the post mortem collected healthy mammary glands of bitches and mammary gland neoplastic tumours obtained during routine surgical treatments. The histopathological examinations revealed that among tumours of epithelial origin, the most frequent were adenocarcinomas, which comprised 64% of all neoplastic lesions. The second group included carcinomas - 26%, whereas 10% were the tumours of mesenchymal origin. The lowest copper content was observed in healthy mammary glands. In the tissues with neoplastic lesions, the content of copper was at a much higher level. Statistical analysis revealed a significant difference (P≤0.05) in the copper content between the investigated groups. The performed analyses showed that mammary gland neoplasms cumulate copper ions, and a much higher concentration of this element is observed in the tumours of epithelial origin.
Effect of copper nanoparticles and copper sulfate administered in ovo on copper content in breast muscle, liver and spleen of broiler chickens. The initial experimental material included 300 hatching eggs of Hubbard Flex chickens. The eggs were divided into three groups: control, NanoCu and CuSO4. Eggs from groups NanoCu and CuSO4 were subjected to in ovo injection to the air cell of egg. Experimental solutions were administered by in ovo injection using a sterile needle and a 0.3 mL syringe as follows: NanoCu – colloid of copper nanoparticles, concentration 50 ppm; and CuSO4 – colloid of copper sulfate, concentration 50 ppm. The eggs were incubated under standard conditions. After hatching, 50 chicks were selected from each group for 42-day rearing. The birds were fed standard feed concentrates for broilers. In that last day of rearing (42 day), 12 females and 12 males were selected from each group. The right part of their breast muscle, their liver and spleen were collected for copper content determinations. Results of this experiment confirm previous scientific reports which demonstrate that the greatest accumulation of copper is observed in soft organs like liver or spleen.
The impact of mineral fertilization with or without multi-component fertilizers on the content of microelements in soil and spring triticale grains was investigated in field trials, in 2009-2011. The experiment was carried out on 8 fertilizing treatments with three replications, which included two varieties of spring triticale: Andrus and Milewo. The content of available zinc and manganese was higher on plots cropped with the cultivar Andrus and nitrogen fertilization with urea or with urea and ammonium nitrate. It was also found out that the content of available manganese, zinc and iron in the analyzed soils was within the natural average range. A higher content of manganese and zinc in grains was detected after the application of multi-component fertilizers. Nitrogen fertilization at a dose of 120 kg.ha-1 together with Azofoska and Ekolist resulted in an increase in the iron content in cv. Andrus. The regression analysis between the content of the analyzed microelements in soil and in triticale grains revealed a significant increase in the iron, manganese and zinc content in grains together with an increase in the content of these elements in soil under cv. Milewo. With respect to the zinc content in soil and in grain from this variety, the coefficient of determination was the closest to the coefficient of a linear correlation (R2 = 0.9105). It was shown that an increase in the content of microelements in soil was not always accompanied by an increase in the content of these elements in spring triticale grains.
The aim of the study was to analyze the total content of micronutrients in the soils in some habitats in the area of the Jezierzyca River Valley Landscape Park. Experimental sites were chosen with regard to different habitats (humid mixed coniferous forest, fresh mixed coniferous forest, humid mixed forest, fresh mixed forest). There are arenosoils, rusty and podzol soils and gley soils. The soils were developed from the river alluvial sands and fluvial sands and prove the acidic reaction. Results showed that the total content of Mn, Zn and Cu is highest in the organic levels and decreases with the depth in the analyzed soils. Only in the podzols soils the total content of Mn decreases in the eluvial horizon and increases in the iluvial horizon. The conducted investigations indicate no pollution of the soils with the micronutrients. The exception was a higher content of Cu in the superficial level of soils in humid and fresh mixed coniferous forest.
The contents of cadmium, lead, zinc and copper were determined in 64 samples of produce (19 strawberries, 16 tops of parsley, 16 dills, 13 lettuces). The produce was gathered directly from home-gardens located near Opole, Wroclaw, Legnica and Jastrzebie Zdroj. The content of cadmium found was within the limits 0.0027–0.663 mg/kg. The contents exceeding the allowable value were found in three samples of dill. The determined quantity of lead ranged from 0.0047 mg/kg to 0.588 mg/kg and the exceeding of allowable contents was found in two dill samples, two lettuce samples and one parsley (greens). The level of zinc contents was between 0.716–34.76 mg/kg. In fourteen samples (3 parsley, 2 lettuces and 9 dills) the limits established before 2004 were exceeded.The contents of copper in produce was within 0.342–17.03 mg/kg. In seven samples (3 parsley and 4 dills) the allowable quantity of Cu established before 2001 was exceeded. The highest amount of cadmium and copper was determined in samples taken near Jastrzebie Zdroj, while the largest quantity of lead and zinc was found in the produce collected in Legnica. The relatively smallest quantities of metals were found in the samples collected near Opole. A comparison of collected data with those available in the literature shows that the recorded level of contamination of the tested produce with heavy metals is close to levels determined for other industrial regions of the country but higher than the world’s standards.
The aim of this study has been to determine the indirect and direct effects of farmyard manure and sewage sludge composts, produced in the north-eastern part of Poland, on the content of 1 mol HCl⋅dm-3 soluble forms of zinc and copper in soil. In 2004-2007, a field experiment was carried out at the Experimental Station in Bałcyny near Ostróda. The experiment was established on proper grey-brown podzolic soil originating from light boulder clay, which was rich in P, moderately abundant in K and low in Mg. It comprised a four-field crop rotation system (potato, spring barley, winter oilseed rape and winter wheat. The design of the experiment, set up according to the random block method, involved 8 objects (2 x 4): 1) farmyard manure, 2) compost (sewage sludge + straw), 3) dried and granulated sewage sludge, 4) composted sewage sludge. The composts and FYM were introduced to soil once (in 2004) at a rate of 10 t d.m.⋅ha-1 or 2 × 5 d.m.⋅ha-1 (under potato and winter oilseed rape). In 2004, nitrogen in the soil enriched with natural fertilizers was balanced to 150 kg⋅ha-1 according to the N-total content. In 2005, soil cropped with spring barley received only mineral fertilization, whereas winter oilseed rape received the second rate of organic fertilizers (in the series consisting of 2 × 5 d.m.⋅ha-1) and nitrogen was balanced to 120 kg⋅ha-1. In 2006, soil under winter wheat received only mineral fertilization. Prior to the establishment of the experiment, soil, manure and compost samples were taken. Having been averaged, the samples were subjected to determination of their content of Cu and Zn in 1 mol HCl dm-3. The soil, whose reaction was 5.04 in 1 mol HCl dm-3, was moderately abundant in available zinc and low in copper. After four years of the trials, the levels of available forms of copper and zinc in the soil fertilized with sewage sludge composts, compared to the soil enriched with FYM, were higher. In the first and fourth year of the experiment, the content of both elements was found to increase significantly in the objects fertilized with dry, granulated and composted sludge. Sludge composted with straw significantly raised the content of Zn and Cu in the second and third year of the experiment. However, fertilization of grey-brown podzolic soil with sewage sludge did not change its classification according to the abundance of available forms of copper and zinc.
The purpose of the study has been to determine the direct and residual effect of farmyard manure and composts made from sewage sludge on the content and uptake of copper and zinc by crops growing in a four-field crop rotation system. In 2004-2007, a field experiment was established on proper grey-brown podzolic soil, originating from light boulder clay, rich in P, moderately abundant in K and low in Mg, whose reaction was pH = 5.04. The experiment involved a four-field crop rotation cycle with the following crops: potato, spring barley, winter oilseed rape and winter wheat. The design of the experiment, set up according to the random block method, consisted of 8 object (2 x 4): 1) FYM, 2) composted sewage sludge, 3) compost (sewage sludge + straw), 4) dried and granulated sewage sludge. The composts and farmyard manure were applied once in the rotation system (under potato) at a rate of 10 t d.m. ha-1 or twice, each time 5 t d.m. ha-1 (under potato and under winter oilseed rape). In the objects fertilized with organic fertilizers and manure, nitrogen was balanced to 150 kg⋅ha-1 (under potato) and to 120 kg⋅ha-1 (under oilseed rape) depending on their total content of nitrogen. Spring barley and winter wheat received only mineral fertilizers. Before the experiment was set up, samples of soil, manure and composts had been collected for determination of Cu and Zn in 1 mol HCl dm-3 by atomic absorption spectrophotometry. The content of zinc and copper in the crops was modified by the form of sewage sludge and sewage sludge composts to a greater extent than by the way these fertilizers were applied in the crop rotation system. Soil fertilization with dried and granulated sewage sludge or composted sludge increased the content of copper and its removal with harvested potato and winter rape, but the analogous values were higher when the cereals were grown on FYM fertilized soil. The strongest influence on the content and removal of zinc was exerted by soil fertilization with dried and granulated sewage sludge. The index of copper and zinc uptake was to a greater degree conditioned by the species of a crop than by the type of fertilizers or their application method. Municipal sewage sludge and sewage sludge composts can be used as a substitute of manure in farms which do not keep farm animals.
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