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1

Determination of Cu, Mn, Zn in some fruit's extracts

100%

Popescu V., Birghila S., Dobrinas S.

Journal of Elementology

|

2005

|

tom 10

|

nr 4 Suppl.1

83

2

Urease activity and ATP content in soil and plant related to copper concentration

100%

Snieg B., Nowak J.

Polish Journal of Ecology

|

2005

|

tom 53

|

nr 1

This study aimed at the assessment of the influence of various Cu(NO₃)₂ doses added to soil on Cu content in soil and on its influence on the activity of urease and ATP content in soil and in plants of various growth stages. A two-factor pot experiment had been started in 2002 using as test plant – the pea (Pisum sativum L.). Soil was taken from 0–30 cm layer of an arable field (light silt loam, 1.2% C content, and neutral reaction). Four doses of copper (II) nitrate (V) as Cu(NO₃)₂. 3H₂O were applied (each in 4 replications) following: I – control (no salt), II – 0.05 mmol. kg⁻¹soil, III – 0.50 mmol kg⁻¹ and IV – 5.00 mmol. kg⁻¹ soil. According to six degrees classification of soil contamination by copper, application of 0.05 mmol of copper nitrate per kg of soil increased copper content up to high level of natural content in soil (but still 0° of contamination), after application of 0.5 mmol. kg⁻¹ – copper content increased to 1° of contamination, 5.00 mmol kg⁻¹ Cu⁺² dose caused high pollution (4° of contamination). Each pot was filled with 2 kg of the treated soil, and 5 pea seeds were planted per pot. The experiment lasted 56 days. Soil moisture was maintained during the experiment at 60% water holding capacity. In the course of the experiment the following growth stages were noted: 2 pairs of leaves stage (day 14th), flowering stage (day 44th), mature stage (day 56th). At those times soil and plant samples were taken to assess copper content in soil (content of total and 1M HCl soluble Cu), urease activity and ATP levels. A high positive correlation was found between Cu content (total and 1M HCl soluble) in soil and in plants. High Cu content in soil (4° of contamination – high pollution) caused a decrease of urease activity and ATP content in soil. Elevated Cu content in plant caused a distinct inhibition of urease activity in all the analyzed growth stages, and markedly higher content of ATP at the stage of flowering and mature stage of Pisum sativum L.

3

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Concentrations of copper, zinc and manganese in potato tubers under the influence of herbicides

86%

Zarzecka K., Gugala M., Baranowska A., Dolega H., Sikorska A.

Journal of Elementology

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2016

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tom 21

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nr 1

A field experiment was carried out in 2008−2010 at the Agricultural Experimental Farm of the University of Natural Sciences and Humanities in Siedlce, located in Zawady. The experiment was established on light loamy soil which belonged to a very good rye complex. The experiment was designed in a two-factor split-plot pattern with three replicates. The factors examined in the experiment included three cultivars of edible potatoes: Cekin, Satina, Tajfun, and five weed control methods: application of the following herbicides (mixed or alone) Command 480 EC, Command 480 EC and Dispersive Afalon 450 SC, Stomp 400 SC, Stomp 400 SC and Dispersive Afalon 450 SC, while the control consisted of mechanical weeding without chemical protection. The aim was to determine the influence of selected herbicides and their combinations (Command 480 EC, Command 480 EC and Dispersive Afalon 450 SC, Stomp 400 SC, Stomp 400 SC and Dispersive Afalon 450 SC) on the content of copper, zinc and manganese in three potato cultivars. Potato tubers were harvested at full maturity. Samples of potato tubers for chemical analyses were taken from plots during harvest. Microelements in the dry matter of potato tubers were determined by the AAS method. The content of copper was significantly dependent on the cultivars, weed control methods and weather conditions during the growing season. In contrast, concentrations of depended on the cultivars and meteorological conditions, whereas the content of manganese in potato tubers was strongly conditioned by genetic traits of the cultivars.

4

Effect of Cu2plus concentration on growth, antioxidant enzyme activity and malondialdehyde content in garlic [Allium sativum L.]

86%

Meng Q., Zou J., Zou J., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

|

2007

|

tom 49

|

nr 1

95-101

The effects of different concentrations (10-5 M, 10-4 M. 10-3 M) of Cu2+ on growth, antioxidant enzyme activity and malondialdehyde (MDA) content were investigated in hydroponically grown Allium sativum L. The results indicated that the growth of garlic seedlings was not inhibited under treatment with 10-5 M Cu2+. Garlic seedlings exposed to 10-4 M and 10-3 M Cu2+ exhibited significant growth reduction. With increasing Cu2+ concentration and treatment time, superoxide dismutase (SOD) activity increased in leaves and roots, and peroxidase (POD) activity increased in leaves. In roots of plants exposed to 10-4 M and 10-3 M Cu2+, POD activity increased within 9 d and then dropped, but was still higher than in the control at the end of the experiment. Catalase (CAT) activity increased in seedlings grown at 10-5 M and l0-4 M, whereas a highly toxic level of Cu2+ (10-3 M) markedly inhibited CAT activity. SOD and POD activity were higher in roots than in leaves, whereas CAT activity was higher in leaves than in roots under both control and Cu2+ treatments. There was no obvious effect on MDA content in the seedlings treated with 10-5 M Cu2+; at 10-4 M and 10-3 M Cu2+ it increased. The mechanisms of Cu2+ toxicity and Cu2+ tolerance in garlic are briefly discussed.

5

Evaluation of zinc and copper concentrations and the total antioxidant capacity of blood plasma in patients with malabsorption syndrome

86%

Kanikowska A., Wlochal M., Mielcarz G., Grzymislawski M., Kucharski M.

Journal of Elementology

|

2015

|

tom 20

|

nr 4

Disturbance of the antioxidative-oxidative balance is a predisposing factor in the development of a variety of diseases. Microelements, including zinc and copper, are components of active enzyme regions participating in anti-oxidative systems. Patients with malabsorption syndrome may suffer from diseases related to the chronic deficiency of micro- and macroelements and vitamins, but also experience health problems due to an impaired antioxidative plasma defence, i.e. illnesses caused by oxidative stress. The aim of the study was to assess zinc and copper concentrations as well as the total antioxidant capacity of blood plasma in 33 patients hospitalized in the Department of Internal Medicine, Metabolic Diseases and Dietetics, presenting symptoms of intestinal malabsorption such as weight loss, abdominal pain and diarrhoea. Blood donors made up the control group. Statistically significantly lower concentrations of zinc and copper were found in the study group as compared to the control group for women and men together as well as for women and for men separately (p < 0.05). A significantly lower FRAP (Ferric Reducing Ability of Plasma) value as a measure of blood plasma the total antioxidative capacity was found in the study group of men in comparison with men from the control group (p < 0.05). Patients showed deficiencies of the two microelements despite normal values of BMI (Body Mass Index) and laboratory parameters such as haemoglobin, protein, albumin and iron concentrations. Regardless of the nutritional state assessment, patients with malabsorption syndrome should receive adequate supplementation of vitamins and microelements.

6

The concentration of Cu in the aquatic plants in Wadag Lake on Olstynian Lakeland

86%

Grzybowski M.

Biological Bulletin of Poznań. Supplement

|

1996

|

tom 33

7

Effect of urea applied with composts on concentration of Cu, Zn and Mn in corn fresh matter

72%

Mazur Z., Sienkiewicz S.

Journal of Elementology

|

2009

|

tom 14

|

nr 2

323-330

Corn was grown for green matter in a pot experiment, in which soil was fertilized with composts alone or in combination with 3 g N per pot (urea). The composts were made in wooden boxes, measuring 50×60×60 cm. They were composted for 3.5 months, until the temperature in the composts became stable and equal to the ambient temperature. Rates of the composts were balanced with amounts of added nitroge, such as 6.0 g N per pot. A one-factor experiment was conducted in Kick-Brauckmann pots, kept in a greenhouse at the University of Warmia and Mazury in Olsztyn. After harvest, fresh matter of stems and leaves as well as corn cobs was determined. Next, the plant samples were dried to determine the dry matter content and finally, after mineralisation, the concentration of Cu, Zn and Mn was determined by atomic absorption spectrophotometry. The composts significantly reduced the concentration of zinc and manganese in the vegetative yield of cor (lstems and leaves). Urea applied in combination with the composts very strongly increased the concentration of manganese and, to a lesser degree, the level of zinc and copper in vegetative organs. In corn cobs, the application of urea increased only the content of manganese. Urea had a stronger effect on increasing the weight of cobs rather than the vegetative mass of corn plants. The uptake of Cu, Zn and Mn was more evidently conditioned by the accumulation of these elements in dry matter than by the total weight of corn plants. The extent of the relationship between the uptake of Cu, Zn and Mn and their concentration of corn biomass is expressed the by corresponding correlation coefficients: 0.66, 0.65 and 0.68.

8

Concentrations of Zn, Pb, Cu, Cd and Ni in the waters of the Narew River and some of its tributaries

72%

Szymczyk S., Grabinska B., Koc-Jurczyk J.

Journal of Elementology

|

2007

|

tom 12

|

nr 3

In the paper, the effects of differences in land use of a catchment and settlements on the variability of heavy metals concentrations (Zn, Pb, Cu, Cd, Ni) in the Narew River and some of its tributaries such as the Biebrza, upper Narew, Pisa, Omulew and Rozoga rivers were studied. The investigations continued from 1997 to 2002. The areas dewatered by the watercources differ considerably in terms of environmental features (forests, wetlands, bogs and water reservoirs), land use (arable lands, grasslands) and population (man-made areas). The total catchment of the Narew River at the cross-section in Zamski Kościelne was also taken into account. The urban areas make up from 0.5% of the Rozoga River to 2.5% of the upper Narew catchment. Water samples were collected quarterly (April, July, October and January) from four study sites located near the river mouth and were analyzed for Zn, Pb, Cu, Cd and Ni by the AAS method. The results showed that concentrations of Pb, Cu, Cd and Ni increased along the Narew River due to the accumulation of the elements in water. A significant influence on high heavy metal concentrations in water was produced by the town of Białystok, a source of large amounts of municipal pollutants discharged into the river. This mainly affected the content of Zn, as the highest pollution with this element was detected in the upper Narew River. The lowest Zn concentrations were observed in the Omulew River water, where forests dominated in the area, covering more than 48% of the catchment. The research showed that wetlands and lakes significantly decreased Pb, Cu, Cd and Ni concentrations in flowing water, but the presence of large inhabited areas with dense population and municipal pollution increased concentrations of heavy metals in river water.

9

Effect of anthocyanin-rich red cabbage extract on cytological injury induced by copper stress in plant and animal tissues

72%

Posmyk M. M., Janas K. M., Kontek R.

Ochrona Środowiska i Zasobów Naturalnych

|

2007

|

nr 33

50-56

10

The effect of nitrogen fertilizer form and foliar application on Cd, Cu and Zn concentrations in carrot

72%

Smolen S., Sady W.

Folia Horticulturae

|

2007

|

tom 19

|

nr 1

11

Changes of copper concentration in blood plasma of kids in neonatal period. The influence of sex and litter size

72%

Ozgo M., Skrzypczak W. F., Herosimczyk A., Kowalska J.

Acta Scientiarum Polonorum. Medicina Veterinaria

|

2007

|

tom 06

|

nr 1

12

Influence of multiple endotoxin injections on Fe, Zn and Cu concentrations in blood serum in horses

72%

Krumrych W., Wisniewski E., Danek J., Gehrke M.

Bulletin of the Veterinary Institute in Puławy

|

2001

|

tom 45

|

nr 2

The aim of the study was to evaluate the Fe, Zn and Cu concentration changes in the course of endotoxin tolerance in horses. The study involved 8 clinically healthy mares subjected to 4 iv. injections with 0.1 μg/kg b.w. of LPS from E.coli - three times at 24 hour intervals (LPS-1, LPS-2, LPS-3) and one injection a week after the last injection (LPS-4). Rectal temperature measurements and blood sampling for testing were performed before each LPS administration as well as 1h, 2h, 3h, 5h and 7h thereafter. It has been found that administration of a relatively small dose of endotoxin produced not only a rise in body temperature but also a significant decrease in Fe and Zn concentration in serum. Subsequent injections (LPS-2 and LPS-3) did not result in significant changes of concentration of these elements, which suggests that endotoxin tolerance mechanisms had been switched on. It has been demonstrated that this process does not last longer than one week because LPS-4 administration resulted in renewed decrease of Fe and Zn concentrations. However, no significant influence of endotoxin injection on Cu content in serum was found.

13

Trace elements and biochemical and haematological parameters in cows with Downer syndrome

58%

Onmaz A., Aytekin I., Aypak S. U., Gunes V., Kucuk O., Ozturk A. S.

Bulletin of the Veterinary Institute in Puławy

|

2011

|

tom 55

|

nr 3

The objective of this study was to investigate the serum concentrations of iron, manganese, cobalt, copper, zinc, and copper/zinc ratio in Downer cows. The study was performed on 20 recumbent Downer cows and 20 healthy cows. The serum concentrations of iron (P=0.002) and copper (P=0.035) were lower in Downer cows compared with the healthy cows. Serum zinc concentrations, although not significantly (P=0.162), were also lower in Downer cows compared with the healthy cows. Cows with Downer syndrome had greater concentrations of serum AST (P<0.001), LDH (P=0.003), and CK (P<0.001). Other biochemical parameters remained unchanged. RBC (P=0.026), Hb (P=0.029), and MCV (P<0.001) in cows with Downer syndrome had lower values compared with those of healthy ones. However, WBC in cows with Downer syndrome had greater values (P<0.00l) compared with those of healthy ones. Other haematological parameters remained similar between Downer cows and healthy cows. It was concluded that serum iron and copper concentrations may play an important role for the aetiology and/or pathogenesis of Downer cows. These elements may be supplemented for the prevention and therapy of recumbency in cows.

14

Antioxidant status, and blood zinc and copper concentrations in dogs with uncomplicated babesiosis due to Babesia canis infections

58%

Teodorowski O., Winiarczyk S., Tarhan D., Dokuzeylul B., Ercan A. M., Or M. E., Staniec M., Adaszek L.

Journal of Veterinary Research

|

2021

|

tom 65

|

nr 2

15

Concentrations of Cd, Pb, Zn, and Cu in roach, Rutilus rutilus (L.) from the lower reaches of the Oder River, and their correlation with concentrations of heavy metals in bottom sediments collected in the same area

58%

Bochenek I., Protasowicki M., Brucka-Jastrzebska E.

Archives of Polish Fisheries

|

2008

|

tom 16

|

nr 1

16

Activity of antioxidant enzymes and concentration of Mn, Cu, Zn and protein in porcine follicular fluid

58%

Lasota B., Ogonski T., Blaszczyk P., Seremak B., Ligocki M., Telesinski A., Kopczynski P.

Acta Scientiarum Polonorum. Zootechnica

|

2011

|

tom 10

|

nr 1

Oxidative metabolism is essential for the gamete and the embryo energy production and is unavoidably associated with generation of reactive oxygen species (ROS). Enzymatic antioxidant defenses are present in the mammalian oocytes, embryos and follicular fluid (FF). An addition of porcine FF to maturation media have positive effects on the IVM and IVF results. The aim of this study was to study the CAT, SOD and GSH-Px activity, as well as Cu, Mn, and Zn concentration in porcine FF collected from the left and the right ovary. The ovaries were collected from 77 gilts at age 8 months. All the analyzed samples of FF revealed active enzymes (24.2·10–3 U · l–1, 2.65·10–3 U · l–1, and 525 U · l–1 for CAT, GSH-Px, and SOD, respectively) and contained Zn, Cu and Mn (13.8·103mol · l–1, 33.3·103 mol · l–1, and 133·10–9 mol · l–1, respectively). In the pFF collected from the left ovary, SOD and GSH-Px activity was higher compared to pFF from the right ovary. On the contrary, the concentration of Cu and Mn was significantly lower in the left ovary pFF. The concentration of Cu ions was negatively correlated with SOD activity. The CAT activity in pFF form left or right ovary did not show any differences.

17

Effect of copper concentration on micropropagation and accumulation of some metals in the Dendrobium kingianum Bidwill orchid

58%

Prazak R., Molas J.

Journal of Elementology

|

2015

|

tom 20

|

nr 3

The study focused on the influence of an increased copper content in the Murashige and Skoog (1962) solid medium on the in vitro plant growth and development of Dendrobium kingianum Bidwill. Sterile explants of pseudobulbs were used for micropropagation of orchid plants on the MS regeneration medium supplemented with 0.5 mg dm-3 NAA and 1.0 mg dm-3 kinetin. Copper (as CuSO4 × 5H2O) was added to all the combinations in concentrations of 0.025 (control), 0.625, 1.25, 2.5 and 5.0 mg dm-3. The results showed that the treatments with 1.25 and 2.5 mg dm-3 stimulated the orchid growth and development in in vitro culture. After eight months of growing in in vitro culture, the highest number of shoots, the longest roots and the heaviest fresh weight of plantlets were obtained in these treatments. In medium with the highest copper concentration (5.0 mg dm-3), a negative influence of the metal on the length of roots and fresh weight of orchids was noted. Spectrophotometric analysis (ASA) showed that the copper and iron accumulation increased in both shoots and roots with the increase in the external Cu level, whereas the zinc and calcium accumulation in these organs decreased. The copper and zinc accumulation in the roots was about 1.5-2.5 times higher than in the shoots, but the iron accumulation was about 3-3.5 times higher. The calcium accumulation in roots was only 5-12% higher than in shoots.

18

Effect of converting enzyme inhibitor on copper and iron concentrations of blood plasma in calves during the neonatal period

58%

Skrzypczak W., Dratwa-Chalupnik A., Ozgo M., Michalek K., Lepczynski A., Hejza K., Siwa J.

Folia Biologica

|

2010

|

tom 58

|

nr 1-2

119-124

19

Zawartość Pb, Zn, Cu w glebach przy drogach o różnym natężeniu ruchu w okolicach Łodzi

58%

Laskowsk S., Toloczko W., Rolka M.

Acta Agrophysica

|

2001

|

tom 56

20

Copper speciation in soils contaminated with metal-bearing dusts

58%

Mocek A., Owczarzak W.

Acta Agrophysica

|

2001

|

tom 56

Ograniczanie wyników

Determination of Cu, Mn, Zn in some fruit's extracts

Urease activity and ATP content in soil and plant related to copper concentration

Concentrations of copper, zinc and manganese in potato tubers under the influence of herbicides

Effect of Cu2plus concentration on growth, antioxidant enzyme activity and malondialdehyde content in garlic [Allium sativum L.]

Evaluation of zinc and copper concentrations and the total antioxidant capacity of blood plasma in patients with malabsorption syndrome

The concentration of Cu in the aquatic plants in Wadag Lake on Olstynian Lakeland

Effect of urea applied with composts on concentration of Cu, Zn and Mn in corn fresh matter