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A field experiment was carried out in 2006-2008 at the Experimental Farm in Zawady, owned by the University of Podlasie. The experimental design was a split-plot arrangement of treatments with three replicates. The factors examined included: factor I – three sowing densities (75, 100 and 125 plants per 1 m2), and factor II – five weed control methods (control – mechanical weed control and four herbicidebased treatments). The objective of the study was to determine the effect of weed control methods as well as sowing density on magnesium and calcium content in the seeds of edible pea (Pisum sativum L.) of Merlin cultivar. The highest seed yield was obtained in the plots where weeds were chemically controlled (Afalon Dyspersyjny 450 SC was sprayed just after sowing and followed by an application of a mixture of Basagran 600 SL + Fusilade Forte 150 EC when plants were 5 cm high). The yield from this treatment was 4.84 t ha–1, on average. The lowest yield was harvested in the plots where weeds were mechanically controlled (the control) – on average 2.92 t ha–1. Variance analysis showed significant influence of weed control methods and weather conditions on magnesium and calcium contents in pea seeds. The herbicides applied in the experiment increased concentrations of the above elements compared with the control. The highest magnesium content (1.389 g kg–1) in pea seeds was found in the plots where Afalon Dyspersyjny 450 SC was applied just after sowing at a dose of 1.5 dm3 ha–1 and followed by a mixture of Basagran 600 SL at a dose of 2.0 dm3 ha–1 + Fusilade Forte at a dose of 1.5 dm3 ha–1 applied post-emergence. The highest calcium content was recorded for treatment 2, consisting of an application of Afalon Dyspersyjny 450 SC at a dose of 1.5 dm3 ha–1 just after sowing and followed by post-emergence spraying with Basagran 600 SL at a dose of 2.0 dm3 ha–1 – on average 0.989 g kg–1. In turn, sowing density had no influence on the discussed characteristics although a tendency was observed towards increasing magnesium and calcium content in edible pea seeds.
Yield and fruit quality of cucumber depend on such factors as plant cultivar, plant nutrition and the cultivation method. The presented paper contains results confirming the usefulness of rockwool and coconut fibre as well as the application of the fertigation system in cucumber growing. Studies on the growing of cucumber Onyks F1 cultivar were carried out in the years 2003–2005 in an unheated greenhouse. A two-factorial experiment was established. One experimental factor consisted in the fertigation control methods including Soltimer device and the starting tray. The other factor included substrate types: rockwool Agroban and coconut fibre Ceres. Plant distribution was 2 plants per one square metre. On the basis of the presented studies, it was found that greenhouse cucumber yielding was influenced by the frequency of nutrient supply, by climatic conditions in the given year and by the applied substrate type. The applied media of rockwool Agroban and coconut fibre Ceres did not exert any influence on plant yielding. On the other hand, a significant effect on the total and the marketable fruit yield was exerted by the cooperation between the fertigation control method and the substrate type.
Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. Universal primers were used for PCR amplification of 464-bp long fragments of the mitochondrial cytochrome b gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, and MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. In 47 samples (78.3%), the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were not great differences between fresh fillets (chilled, frozen etc.) and heat-processed foodstuffs (tinned, smoked, etc). The correct designation was in 72.3% and 81.6% of samples, respectively. Even if in three cases the analysis was unsuccessful the method is useful for the control of the adulteration of food with fish tissue content and in this way it contributes to better consumer awareness.
The aim of the study was to evaluate a possibility of propolis production by honey bee Apis mellifera (Horn) (Hymenoptera: Apidae) and its potential for the management of the larger grain borer (LGB) Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) on maize grains. Bee propolis was collected from hives kept on the field for twelve months; they were thereafter diluted with ethanol to obtain 5%, 10%, 15% and 20% concentrations. 5 ml each of the concentrations was applied to 150 g clean maize grains infested with 10 pairs of 1-5 days old LGB in 250 cm3 Kilner jars. The control jars were treated with ethanol. At 90 days post-infestation, data were collected on percentage of weight loss, percentage of grain damage, weight of frass generated (g), number of pupae, larvae and adult LGB. The results indicated that monthly propolis yield was significantly (p < 0.05) lower in the period of honey flow (December-March) relative to other periods. Propolis at all the concentrations tested reduced the population of LGB in propolis-treated maize grains relative to non-propolis treated-ones. The highest effects of propolis was shown at the highest concentration of 20% and it was significantly (p < 0.05) different from its effects at other concentrations. Bee propolis may be integrated with other ecological friendly control methods to manage LGB infestation in maize.
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