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Theaimof the present paper was to define the qualitative and quantitative composition of phenolic compounds in the extracts of fulvic acids and hydrolyzates of humic acids isolated from forest soil. Forest soil was sampled from the organic horizon (5-3.5 cm – sub-horizon Ol and 3.5-0 cm– sub-horizon Ofh) and mineral horizon every 10 cm (0-50 cm). Phenoliccompounds in fulvic acids (FAs) extracts and humic acids (HAs) hydrolyzates were identified by high performance liquid chromatography (HPLC). The recorded chromatograms showed differences in the composition of phenolic compounds between humic and fulvic acids fractions. The content of those compounds both in extracts of fulvic acids and hydrolyzates of humic acids and V+S+C parameter values decreased together with the sampling depth. The highest values of V+S+C parameter were calculated for extracts of fulvic acids and humic acids hydrolyzates isolated from the organic horizon of forest soil. The share of the vanillyl type in the extracts of humus acids (HAs+FAs) of the subhorizon Ol was close to that of the sum share of the syringyl and cinnamyl type. The greater the depth, the greater the share of vanillyl compounds in the pool of the share of syringyl, cinnamyl and vanillyl compounds.
The aim of the study has been to determine the effect of some substances such as dolomite, loam, compost, pinewood bark, peat, lime, charcoal, natural and synthetic zeolite on reducing the impact of soil contamination with arsenic on the content of manganese in some plant species. The content of manganese in the test plants depended on the degree of soil contamination with arsenic, application of different substances as well as on the plant species and organ. Soil contamination with arsenic caused either an increase or a decrease in the content of manganese in plants depending on a plant species and organ. In the series without soil amending substances, in the arsenic contaminated objects the manganese content decreased in above-ground parts of cocksfoot and swede but increased in above-ground parts and roots of maize and yellow lupine, in roots of cocksfoot and swede and in straw and roots of spring barley. On the other hand, the highest rates of arsenic depressed the content of manganese in roots of cocksfoot, swede and spring barley. Addition of any of the aforementioned substances to contaminated soil changed the content of manganese in the plants. The most unambiguous effect of the different substances was determined in the case of above-ground parts of maize as well as above-ground parts and roots of cocksfoot, in which the manganese content fell down, and in roots of yellow lupine, grain and straw of spring barley, in which the content of manganese rose. Charcoal and loam caused the largest and synthetic zeolite led to the smallest changes in the content of manganese in plants.
A single dose of 10, 25 and 50% (weight percentage) of the soil collected from sugar-processing plant was added to loose sand and brown soil. A four –year - pot experiment was set where the following were cultivated, in turn: corn, buckwheat, spring wheat and corn. It was observed that the content of phenolic acids in soil grew proportionally to the dose of soil introduced and it was positively correlated with the contents of C-org, N-org, available P, K and Mg forms, floatable forms and sorption capacity, as well as the plant yield and its nitrogen and magnesium contents. However, the content of phenolic acids in brown soil did not show a linear dependence on the sediment dose, it correlated negatively with the amount of C fraction most susceptible to biological oxidation and with microbiological respiration activity, yet positively with the concentration of potassium available in soils, yield and its nitrogen content.
Background. Meat products belong to products particularly at risk of fat oxidation processes. One of the methods to prevent disadvantageous oxidative changes of lipids in food is the application of antioxidants. Material and methods. The experimental material consisted of fine - ground model sausages. Produced processed meats differed in terms of the presence and amount of acid and enzymatic soy hydrolysates (0.3% and 0.7%). The reference sample comprised processed meat product with no hydrolysate added. Model processed meat products were stored at 4°C for 29 days. The analyses included changes in peroxide value, changes in cholesterol and its oxidation products. Results. It was found that changes of peroxide value, 7α-OHC, 7β-OHC, α-epoxy-C, β-epoxy-C, 20α-OHC, 25-OHC and total oxisterols were statistically significantly af-fected, apart from storage time, also by the type and level of applied hydrolysates. The addition of enzymatic and acid hydrolysates to batter of experimental sausages effectively inhibited the process of fat oxidation. Conclusions. In samples with enzymatic hydrolysate an approx. 20% loss of initial cholesterol content was recorded. In contrast, in the other samples this loss amounted to approx. 10%. The process of cholesterol metabolism in tested processed meat products was affected by their storage time and the type of added hydrolysate. It was observed that the highest dynamics of cholesterol metabolism occurred in a sample with no hydrolysate added. The level of total oxisterols in the sample with no addition of hydrolysate was over two times higher than in samples with an addition of hydrolysate.
The aim of the present study was to examine the influence of spectral composition of irradiation (white - WL or white supplemented by blue radiation - WBL) and ozone on chlorophyll content and the optical properties of broccoli leaves (Brassica oleracea var. botrytis cymosa). Broccoli leaves were injured by the ozone dose applied (70 ppb, 6 hours daily) mainly under WBL. Leaf lamina damage was much larger in the outer, physiologically older part than in the inner, physiologically younger part. Therefore, further studies were carried out separately on leaf fragments from the outer(OP) and the inner parts (IP). The spectrophotometric analysis did not reveal significant differences in the chlorophyll content between treatments. The variations in reflectance in the visible region (400-700 nm) were generally not significant with respect to tissue age (OP, IP), ozone fumigation and growth conditions (WBL, WL). Only in plants grown under WBL there was an increase in green reflectance in ozone-treated leaves observed, which indicates a decrease in the chlorophyll content in leaves. Thus, measurements of the optical properties of leaves provide more precise information about changes in the content of chlorophyll than spectrophotometry. Leaves under WBL showed significant differences in reflection within the range 800-1100 nm, which depended on the physiological age of the tissue. For physiologically older tissues, values of reflection were lower than those for physiologically younger tissues. However, under WL conditions, a decrease in reflection within the 800-1100 nm rangę depended only on ozone fumigation. Response of broccoli leaves to spectral composition of irradiation and ozone stress was much higher for irradiation transmission than for reflection. Compared to the control, ozone fumigation of broccoli leaves grown in WBL increased irradiation transmission in OP and IP leaf fragments, both in the 500-700 nm and 750-1100 nm ranges. For plants growing in WL no influence of ozone fumigation on the transmission of irradiation within the visible range was observed. However, fumigation with ozone resulted in a significant decrease in the transmission within the infrared range (750 to 1100 nm). The results indicate the major role of irradiation spectral composition in plant response to ozone stress. The modifications may affect Chl content, leaf temperature and, what is more important, phytochrome-controlled morphogenetic processes.
The aim of the study was to assess the effect of the introduction of curing brine, massaging and pasteurisation on the dynamics of changes in collagen content and its thermohydrolysis rate in two bovine muscles with different topographic positions in the carcass: the semimembranosus muscle (m. semimembranosus) and the lumbar part of the longissimus dorsi muscle (m. longissimus lumborum). Brine contained, among other things, NaCl, phosphates and collagen protein. In the study the pH value of muscles, contents of crude protein and dry matter were determined together with the amount of collagen and its susceptibility to thermohydrolysis; moreover, shear force value was determined using the WBSF test. On the basis of the conducted tests it was found that the semimembranosus muscle contained more collagen than the lumbar part of the longissimus dorsi muscle. The introduction of brine, massaging and pasteurisation resulted in changes not only in its amount, but also in its susceptibility to thermohydrolysis. Curing resulted in a decreased rate of collagen thermohydrolysis in both analysed muscles in comparison to raw muscles: from several times in m. semimembranosus to around fifteen times in m. longissimus lumborum in terms of crude protein and over twenty times in relation to the total collagen content. The rate of collagen thermohydrolysis in both muscles varied: it was higher in m. semimembranosus than in m. longissimus lumborum. The most profound effect on changes in tenderness (test WBSF) was found for massaging, which in the semimembranosus muscle resulted in its decrease, while in the lumbar part of the longissimus dorsi muscle it caused an increase in comparison to the value obtained after the introduction of brine. It was found that after pasteurisation the dorsal muscle was more tender in comparison to the semimembranosus muscle in spite of the fact that it was characterised by a higher share of collagen in the total amount of protein.
We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA–). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA– leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA- leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.
Badania przeprowadzono na 2 grupach indorów typu Big-6 podczas 16-tygodniowego odchowu. Czynnikiem doświadczalnym był dodatek miedzi w formie chelatu z lizyną w ilości 10 mg Cu-dm-3H2O. Celem badań było określenie wpływu miedzi jako dodatku do wody pitnej na masę ciała oraz kumulację składników mineralnych w wybranych tkankach indyków. Wykazano, że dodatek chelatu spowodował zwiększenie końcowej masy ciała indorów, natomiast nie wpłynął w znaczący sposób na masę analizowanych tkanek. Stwierdzono, że zastosowanie Cu wpłynęło na wzrost zawartości Cu, Zn i Fe we wszystkich badanych tkankach. Największą retencję Cu wykazano w przypadku wątroby, natomiast najmniejsze ilości tego pierwiastka kumulowały się w mięśniach piersiowych ptaków. Długotrwała aplikacja miedzi nie spowodowała nadmiernej kumulacji tego składnika w nerkach i mięśniach indyków, co nie stanowiło zagrożenia dla zdrowia konsumentów, gdyż jej zawartość nie przekraczała dopuszczalnych norm (5 mg-kg-1; wg PN-A-86524:1994).
Although some small amounts of mercury in surface water come from natural environment, much higher quantities originate from anthropogenic sources, including industry and agriculture. Mercury readily accumulates in bottom sediments, from which it can return to water, which can be a serious cause of mercury pollution even after other mercury sources are removed from a river’s drainage basin. Concentration of mercury was examined in river waters flowing from basins characterised by different land use and population density. Samples of water were collected from two right tributaries of the Narew River, called the Pisa and the Biebrza, and at six sites in the middle section of the main river. The areas drained by these three rivers lie within the ecosystem known as the Green Lungs of Poland. It was found that the average mercury concentration in the waters of the Narew and its tributaries ranged between 0.3 and 0.9 pg dm-3, being only slightly higher than the concentrations typical of unpolluted territories. The highest mercury content occurred in spring and in the waters from agricultural-forested basins with large towns. Changes in the mercury outflow were associated with accumulation of household pollutants downstream, atmospheric conditions as well as the content of organic matter and suspended solids in the river waters. Low concentration of mineral suspension and organic substance carried by the Pisa River significantly depressed the mercury content in the water of this river. In the Biebrza River, the concentration of both organic and mineral substance was directly proportional to the content of mercury.
Changes in content of cyanogenic glycosides and in cyanogenesis potential within the bird cherry leaves during occurrence of the bird cherry-oat aphid, Rhopalosiphum padi L. and while its spring host-plants alternation have been studied. The highest content of the cyanogenic glycosides and the highest cyanogenesis potential was found in the youngest leaves of the primary host during occurrence of the first fundatrices. When the aphid population started to build up, a decrease in content of the cyanogenic glycoside and in the cyanogenesis potential was observed. Finally, when the winged migrants began to fly off from the primary host onto cereals, pretty low amount of the plant xenobiotics was recorded within the bird cherry leaves. Possible role of the cyanogenesis in host alternation of the bird cherry-oat aphid is discussed.
Sok z mieszaniny jabłek zawierającej 80% odmiany ‘Szampion’ (wysoka zawartość związków fenolowych i wysoka aktywność anty oksydacyjna) i 20% odmiany ‘Lobo’ (wysoka aktywność oksydazy polifenolowej) charakteryzował się w czasie przechowywania wyższą stabilnością fizyczną i biochemiczną w porównaniu z sokami jednorodnymi, otrzymywanymi z tych samych odmian jabłek. Sugeruje się, że przyczyną tych różnic jest, indukowany aktywnością oksydazy polifenolowej na etapie otrzymywania soku mieszanego, spadek zawartości kwasu chlorogenowego i epikatechiny. W czasie rocznego przechowywania soku mieszanego, w przeciwieństwie do soków jednorodnych przechowywanych przez 5 miesięcy, jego aktywność przeciwutleniająca pozostawała na tym samym poziomie, natomiast zmniejszenie zawartości związków fenolowych charakterystycznych dla jabłek było zauważalnie mniejsze i dotyczyło głównie kwasu chlorogenowego.
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