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1

Recombinant strains of Escherichia coli for L-aspartic acid biosynthesis

100%
Gadomska G., Plucienniczak A., Chmiel A.
Polish Journal of Microbiology
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2007
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tom 56
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nr 2
The aspartase overproducing mutant B-715 was used as a donor of the aspartase gene for further construction of the aspartase-hyperproducing strains by molecular cloning. In preliminary experiments activity of transformants and their efficiency in L-aspartic acid biosynthesis were compared. The conditions for recombinant strain multiplication, biomass activation and L-aspartic acid biosynthesis were optimized. The optimum temperature for cells multiplication, their activation and for product biosynthesis was 37°C. Two-stage process of the multiplication of bacteria (first in LB medium, and then in FF medium) eliminates the appearing of the inclusion bodies of aspartase in the cells. The shaking during cell activation improved cells productivity. The change of pH in the course of the biosynthesis process was insignificant but did not influence the process.
2
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Optimisation of conditions for capillary zone electrophoresis [CZE] separation of trichloroacetate extracts of salted fish meat

84%
Felisiak K.
Polish Journal of Food and Nutrition Sciences
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2007
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tom 57
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nr 2
177-184
Effects of CE parameters and modifying substances on the quality of separation of trichloroacetic (TCA) extracts of salted Baltic herring meat were studied. The optimal separation parameters were as follows: capillary length 36 cm; voltage 20 kV; injection pressure 2 psi*s; capillary temperature 35°C, detection wavelength 200 nm. Buffer enrichment with 20% acetonitrile and 40% methanol resulted in a slight improvement of the separation quality, while 20 mmol/L triethylamine (TEA) produced some improvement, but was a source of an additional peak in field A. Salt concentration
3

High density solid biofuel from oil palm biomass

67%
Chin K. T., H'ng P. S., Maminski M.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
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2012
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tom 77
High density solid biofuel from oil palm biomass. Large quantities of oil palm biomass available can significantly become the important source for renewable energy in Malaysia in the form of pellet or briquette. Nonetheless, lignocellulosic biomass should be treated to increase their energy efficiency prior to the combustion. Therefore, the aim of this study was to investigate the effects of torrefaction treatment on the weight loss and energy properties of oil palm biomass (oil palm trunk and empty fruit bunch). The lignocellulosic biomass was torrefied at three different temperatures (200 to 300 °C) within 15 to 45 min. Response surface methodology (RSM) was used for optimization of torrefaction conditions, to determine the maximum energy properties and minimum weight loss. Results showed that heating values of biomass was affected by treatment severity (cumulated effect of temperature and time). While the reaction temperature had a strong impact on the energy density of torrefied biomass, the effect of treatment time was considerably lesser under the torrefaction conditions used in this study. It was demonstrated that each biomass type had its own unique set of operating conditions to achieve the same product quality. The optimized torrefaction conditions were verified empirically and applicability of the model was confirmed. The results of this study could be used as a guide for the production of high density solid biofuel from oil palm biomass. Conclusively, high density solid biofuel from oil palm biomass can be produced using torrection method.
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