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1

Effect of extracts of St. John's wort (Hypericum perforatum L.) and lemon balm (Melissa officinalis L.) herbs on local GvH reaction and production of anti-SRBC antibodies

100%
Skopinska-Rozewska E., Rzadkowska-Bodalska H., Olechnowicz-Stepien W., Zukowska M., Malkowska-Zwierz W., Radomska D., Sobczynska A.
Bulletin of the Polish Academy of Sciences. Biological Sciences
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1991
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tom 39
|
nr 3
2

Prevalence of Chlamydia suis in population of swine in Poland and comparison of complement fixation test and PCR used in the diagnosis of chlamydiosis

100%
Szymanska-Czerwinska M., Niemczuk K., Wojcik A.
Bulletin of the Veterinary Institute in Puławy
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2011
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tom 55
|
nr 3
The 61,020 samples of swine serum and vaginal swabs were collected in 2007-2009. The sera were examined by complement fixation test (CFT). Positive results were found in 277 pigs. The percentage of positive serological results in subsequent years was: 0.4%, 0.47%, and 1.65%, respectively. The vaginal swabs (n=277) selected from pigs diagnosed as positive in the CFT were tested by PCR. Occurrence of Chlamydia suis was confirmed in PCR in 200 cases. Statistical analysis (χ² test, Person and Cramer correlation coefficient) demonstrated that both methods were coincident in the diagnosis of C. suis infection in swine.
3

Evaluation of agreement of ELISA and complement fixation test in the diagnostics of Q fever in cattle

100%
Jozwik A., Jakubowski T., Kaba J., Jurkowski W., Witkowski L., Nowicki M., Frymus T.
Medycyna Weterynaryjna
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2007
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tom 63
|
nr 06
655-657
Q fever is a worldwide zoonosis that is manifested as a reproductive failure in animals and by polymorphic, nonspecific symptoms in humans. The infection may be acquired through the respiratory or alimentary route or an arthropod bite. The diagnosis of Q fever relies mainly upon serology: indirect immunofluorescence assays (IFA), complement fixation (CF) tests or enzyme-linked immunosorbent assays (ELISA) are used to detect antibodies against Coxiella burnetii in sera of infected animals. The aim of our study was to evaluate the agreement of the commercially available ELISA kit and CF test using statistical methods (Kappa value). We used serum samples that were collected from 122 dairy cows from one herd in central Poland. The general health status of the herd was good, and the animals were clinically normal. Our results showed a low agreement (Kappa value = 0.376) between the commercially available ELISA and CF test.
4

Application of ELISA microplate reader in complement fixation test used for dourine diagnosis. A preliminary study

100%
Cencek T., Karamon J., Ziomko I., Sroka J.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
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nr 3
The aim of the present work was to estimate the possibility of improving the objectiveness of complement fixation test (CFT) in the diagnosis of dourine by using an ELISA microplate reader. The investigations were carried out on control positive and negative sera and 16 sera obtained for a reference examination. Six modifications of CFT performing and reading methods were compared (tube, microplate method, and microplate method with double volume of reagents in combination with visual and spectrophotometrical readings). The linear regression functions were calculated for the estimation of the percentage of haemolysis in CFT reaction on the basis of supernatant absorbance for the methods in which spectrophotometer or ELISA reader were used. High level of correlation of the results obtained by different methods was achieved. All coefficients of the correlation were higher than 0.9 and were statistically significant in each case. The investigations showed that it is possible to use the ELISA microplate reader for dourine diagnostics by CFT.
5

Agreement between ELISA and complement fixation test used for diagnosing of paratuberculosis in goats

80%
Kaba J., Gerlach G. F., Nowicki M., Rypula K.
Polish Journal of Veterinary Sciences
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2008
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tom 11
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nr 3
209-212
An ELISA with a iipoarabinomannan as an antigen, developed for diagnosis of bovine paratuber- culosis, has been adapted for use in goats, and compared with complement fixation test. Kappa value of 0.62 indicated good agreement between CFT and the adapted ELISA and proved that the investigated ELISA may be helpful in diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats. The ELISA has been used to screen a randomly selected representative sample of Polish breeding goat population (21.78% of herds, 21.33% of goats). It has been demonstrated that only 2.42% of animals coming from 15.79% of herds were seropositive. Within-herd seroprevalence varied from 1.69% to 38.10%. Most of the infected animals (67.07%) were 3-4 years old. No seropositive cases were found in group up to 1 year old animals.
6

Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay

80%
Weiner M., Iwaniak W., Zlotnicka J., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2010
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tom 54
|
nr 4
The aim of this study was the application of fluorescence polarisation assay (FPA) for the examination of bovine sera and comparison of the results of the assay with the results of Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), and ELISA. Six hundred thirty-five sera from cattle, including 300 sera from healthy animals, 32 sera from animals regarded as serologically positive for brucellosis and culled, and 303 sera originated from confirmatory investigations were used. All sera originating from healthy animals, negative in ELISA, RBT, SAT, and CFT were also negative in FPA. Among 303 sera from confirmatory investigations, 269 were positive in both RBT and SAT, 21 were positive in SAT and remaining 13 were RBT-positive only. Only two sera, one positive in both tests (RBT, SAT) and one SAT-positive, were also positive in FPA. Among 32 sera originated from animals regarded as serologically positive, which reacted in RBT, SAT, and CFT, 14 gave positive results in ELISA, whereas 18 were negative. Among these ELISA-positive sera, 13 were also positive in FPA. All samples positive in SAT, RBT, and CFT, and negative in ELISA, were also negative in FPA.
7

Evaluation of the ELISA in diagnosis of bovine brucellosis. Part I - Examination of sera

80%
Szulowski K.
Polish Journal of Veterinary Sciences
|
1998
|
tom 01
|
nr 2
The investigation aimed at preparing an ELISA kit for examination of bovine sera for brucellosis and evaluating the diagnostic properties of the test. Lipopolysaccharide extract (LPS) was used as the antigen for microplate coating, antibodies against IgG of bovine sera with horseradish peroxidase were used as the conjugate and ABTS with H202 as the substrate. A weak-positive serum prepared from the second International Standard of anti-Brucella abortus Serum (ISaBaS II) and a negative serum obtained by mixing sera from Brucella-free cows were used as controls. Evaluation of the diagnostic usefulness of the ELISA was performed by comparison of the results obtained with those in the rose bengal test (RBT) and complement fixation test (CFT). The examinations involved 212 bovine sera positive in the RBT, 60 sera positive in the CFT, 5 standard sera, and 1005 sera negative in the RBT. A high correlation was found between the ELISA and CFT results (55 sera out 60 sera) and no correlation between the ELISA and RBT results. Only 60 out of 212 sera positive in RBT gave positive results in the ELISA. All standard sera were positive in the ELISA and only 1 serum from those negative in the RBT was doubtful in the ELISA. The ELISA proved to be a suitable method for diagnosis of brucellosis in cattle.
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