Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The protective ability of the Mediterranean plant extracts against the oxidative DNA damage. The role of the radical oxygen species and the polyphenol content

100%

Kapiszewska M., Soltys E., Visioli F., Cierniak A., Zajac G.

Journal of Physiology and Pharmacology. Supplement

|

2005

|

tom 56

|

nr 1

183-197

The polyphenol plant extracts content seems to be responsible for the scavenging activity of the reactive oxygen species (ROS), resulting in protection against DNA damage induced by the oxidative stress. This assumption was verified analyzing the effect of six Mediterranean plant extracts (Crepis vesicaria L, Origanum heracleoticum, Scandix australis L, Amaranthus sp., Scolymus hispanicus L, Thymus piperella L) on the oxidative DNA damage induced in lymphocytes by H2O2 in relation to the polyphenolic content and the lymphocyte scavenging ability of ROS. The comet assay was used to evaluate oxidative DNA damage and the polyphenol content was analyzed by the Folin-Ciocalteu method. The fluorescence resulting from oxidation of ROS-sensitive dye, dihydrorofluorescein (DHF), was utilized as indicator of the ROS level. Pretreatment with all plant extracts produced the dose-dependent increase in the DNA protection up to the 0.2 µg/ml polyphenol content and the decrease above that dose. Only the Thymus piperella, similarly to quercetin, showed a strong positive correlation between the DNA protection and the polyphenol content, but negative correlation with ROS formation. In conclusion, the DNA protective ability of plant extracts seems to be related to the low polyphenol concentration and only to certain extent depends on the polyphenol ROS scavenging activity.

2

Elektroforeza pojedynczych komorek [comet assay] - uzyteczna technika badania uszkodzen DNA

100%

Jaloszynski P., Kujawski M., Szyfter K.

Postępy Biologii Komórki

|

1996

|

tom 23

|

nr 3

339-354

3

Is concentration and motility of male gametes related to DNA damage measured by comet assay?

100%

Dobrzynska M. M., Tyrkiel E. J., Derezinska E., Ludwicki J. K.

Annals of Agricultural and Environmental Medicine

|

2010

|

tom 17

|

nr 1

73-77

4

Structural damage to nuclear DNA in mammalian spermatozoa: its evaluation techniques and relationship with male infertility

100%

Fraser L.

Polish Journal of Veterinary Sciences

|

2004

|

tom 07

|

nr 4

Diagnosis of the fertilizing ability of a semen sample is important for consistently high reproductive efficiency. Disturbances in the organization of the genomic material in sperm nuclei can have a serious impact on the growth of the offspring, therefore a stable nuclear matrix is crucial for participation in embryonic development. Routine semen analysis investigates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin structure or damaged DNA in spermatozoa is implicated as a possible cause of increased infertility in males. Therefore, it is crucial to develop and use accurate and diagnostic tests, which may provide better prognostic capabilities than the standard sperm assessments. This article reviews and discusses some of the current techniques employed for evaluating chromatin structure or DNA damage in spermatozoa. These different techniques include the comet assay, sperm chromatin structure assay (SCSA), acridine orange test (AOT), tritium-labelled 3H-actinomycin D (3H-AMD) incorporation assay, terminal TdT-mediated dUTP-nick-end labelling (TUNEL) assay, in-situ nick translation (ISNT) assay, DNA breakage detection-fluorescence in-situ hybridizations (DBD-FISH) assay and sperm chromatin dispersion (SCD) test. The aforementioned assays, which are considered independent measure of sperm quality, may help to detect subtle defects in the chromatin structure or DNA integrity, and thereby assist in semen quality assessment. The relationship between DNA damage and male infertility is also addressed.

5

Differential resistance of human embryonic stem cells and somatic cell types to hydrogen peroxide-induced genotoxicity may be dependent on innate basal intracellular ROS levels

84%

Vinoth K. J., Manikandan J., Sethu S., Balakrishnan L., Heng A., Lu K., Poonepalli A., Hande M. P., Cao T.

Folia Histochemica et Cytobiologica

|

2015

|

tom 53

|

nr 2

6

Methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies

84%

Hoelzl C., Bichler J., Ferk F., Simic T., Nersesyan A., Elbling L., Ehrlich V., Chakraborty A., Knasmuller S.

Journal of Physiology and Pharmacology. Supplement

|

2005

|

tom 56

|

nr 2

49-64

It is well documented that reactive oxygen species (ROS) are involved in the aetiology of age related diseases. Over the last decades, strong efforts have been made to identify antioxidants in human foods and numerous promising compounds have been detected which are used for the production of supplements and functional foods. The present paper describes the advantages and limitations of methods which are currently used for the identification of antioxidants. Numerous in vitro methods are available which are easy to perform and largely used in screening trials. However, the results of such tests are only partly relevant for humans as certain active compounds (e.g. those with large molecular configuration) are only poorly absorbed in the gastrointestinal tract and/or may undergo metabolic degradation. Therefore experimental models are required which provide information if protective effects take place in humans under realistic conditions. Over the last years, several methods have been developed which are increasingly used in human intervention trials. The most widely used techniques are chemical determinations of oxidised guanosine in peripheral blood cells or urine and single cell gel electrophoresis (comet) assays with lymphocytes which are based on the measurement of DNA migration in an electric field. By using of DNA-restriction enzymes (formamidopyrimidine DNA glycosylase and endonuclease III) it is possible to monitor the endogenous formation of oxidised purines and pyrimidines; recently also protocols have been developed which enable to monitor alterations in the repair of oxidised DNA. Alternatively, also the frequency of micronucleated cells can be monitored with the cytokinesis block method in peripheral human blood cells before and after intervention with putative antioxidants. To obtain information on alterations of the sensitivity towards oxidative damage, the cells can be treated ex vivo with ROS (H2O2 exposure, radiation). The evaluation of currently available human studies shows that in approximately half of them protective effects of dietary factors towards oxidative DNA-damage were observed. Earlier studies focused predominantly on the effects of vitamins (A, C, E) and carotenoids, more recently also the effects of fruit juices (from grapes, kiwi) and beverages (soy milk, tea, coffee), vegetables (tomato products, berries, Brussels sprouts) and other components of the human diet (coenzyme Q10, polyunsaturated fatty acids) were investigated. On the basis of the results of these studies it was possible to identify dietary compounds which are highly active (e.g. gallic acid). At present, strong efforts are made to elucidate whether the different parameters of oxidative DNA-damage correlates with life span, cancer and other age related diseases. The new techniques are highly useful tools which provide valuable information if dietary components cause antioxidant effects in humans and can be used to identify individual protective compounds and also to develop nutritional strategies to reduce the adverse health effects of ROS.

7

The estrogenic status and the COMT genotype of female blood donors influence the protective ability of the Mediterranean plant extracts against the hydrogen peroxide-induced DNA damage in lymphocytes

84%

Kapiszewska M., Zajac G., Kalemba M., Soltys E.

Journal of Physiology and Pharmacology. Supplement

|

2005

|

tom 56

|

nr 1

199-217

The extent of oxidative DNA damage in lymphocytes can be used as a biomarker of the level of oxidative stress in the body. The comet assay has been widely used to measure such damage. The aim of our study was to evaluate: i) the extent of the oxidative DNA damage in lymphocytes isolated from blood of female donors taken in early and late follicular phases [low (LE) and high (HE) concentration of 17bestradiol, respectively], ii) the susceptibility of these lymphocytes to hydrogen peroxide exposure, and iii) the protective ability of five plant extracts against the hydrogen peroxide-induced DNA damage. The effect of the catechol-Omethytransferase genotype (wild COMT H/H and mutated homozygote COMT L/L) of female donors was also analyzed. The amount of endogenous DNA damage was higher in HE lymphocytes as compared with LE ones, independently of the genotype. When lymphocytes were stratified by COMT genotype, the level of DNA damage was higher in L/L donors. The protective effect of pretreatment with plant extracts (1 and 10 µg/ml for 1 h) against the H2O2 (25 µM, 5 min. at 4°C)-induced oxidative DNA damage was observed only in H/H HE lymphocytes. In contrary, the plant extract pre-incubation enhanced the DNA damage in L/L HE lymphocytes. The plant extracts alone did not induce the DNA damage. The results showed that concentration of the circulating 17b-estradiol influenced the extent of endogenous oxidative DNA damage while the beneficial or hazardous effects of the plant extracts might depend on the COMT genotype and the estrogen level.

8

Spontaneous DNA damage in lymphocytes of fox species

84%

Grzesiakowska A., Szeleszczuk O., Kuchta-Gladysz M., Otwinowska-Mindur A.

Folia Biologica

|

2019

|

tom 67

|

nr 1

9

Neuroprotective effects of onion and garlic root extracts against Alzheimer’s disease in rats: antimicrobial, histopathological, and molecular studies

84%

Hegazy E. M., Sabry A., Khalil W. K. B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2022

|

tom 103

|

nr 2

10

DNA damage in mouse lymphocytes exposed to curcumin and copper

84%

Urbina-Cano P., Bobadilla-Morales L., Ramirez-Herrera M. A., Corona-Rivera J. R., Mendoza-Magana M. L., Troyo-Sanroman R., Corona-Rivera A.

Journal of Applied Genetics

|

2006

|

tom 47

|

nr 4

Dietary polyphenolics, such as curcumin, have shown antioxidant and anti-inflammatory effects. Some antioxidants cause DNA strand breaks in excess of transition metal ions, such as copper. The aim of this study was to evaluate the in vitro effect of curcumin in the presence of increasing concentrations of copper to induce DNA damage in murine leukocytes by the comet assay. Balb-C mouse lymphocytes were exposed to 50 µM curcumin and various concentrations of copper (10 µM, 100 µM and 200 µM). Cellular DNA damage was detected by means of the alkaline comet assay. Our results show that 50 µM curcumin in the presence of 100-200 µM copper induced DNA damage in murine lymphocytes. Curcumin did not inhibit the oxidative DNA damage caused by 50 µM H₂O₂ in mouse lymphocytes. Moreover, 50 µM curcumin alone was capable of inducing DNA strand breaks under the tested conditions. The increased DNA damage by 50 µM curcumin was observed in the presence of various concentrations of copper, as detected by the alkaline comet assay.

11

Single cell gel electrophoresis [comet assay] as a tool for environmental biomonitoring. An example of pesticides

84%

Blasiak J., Trzeciak A.

Polish Journal of Environmental Studies

|

1998

|

tom 07

|

nr 4

Methods that permit the sensitive detection of DNA damage and repair are of special significance in the field of environmental research due to the long latent period between exposure to environmental agent(s) and genetic effect(s) becoming apparent. Malathion is a commonly used organophosphorus insecticide reported to be genotoxic both in vivo and in vitro, but the reports are conflicting. In order to elucidate the genotoxic potency of a compound present in commercial preparations of malathion, the DNA damaging effect of the insecticide and its isomer isomalathion was investigated using alkaline single cell gel electrophoresis (comet assay). Freshly isolated human peripheral blood lymphocytes were incubated with 75 and 200 µM of the chemicals for 1 h. The concentrations from the range we used, is relevant to that in blood following various nonlethal human exposure to pesticides. Malathion did not cause any significant changes in the comet length of the lymphocytes throughout the range of concentrations tested. Isomalathion introduced damage to DNA in a dose-dependent manner. Treated cells were able to recover within the 60 min incubation in insecticide-free medium at 37°C. The reported genotoxicity of malathion might, therefore, be a consequence of its thermal and/or photochemical conversion to isomalathion and the presence of isomalathion as well as its oxidation products and other unspecified impurities in commercial formulations of malathion. In this regard, the results of our study indicate that malathion used as commercial product, i. e. containing isomalathion, can be considered as a genotoxic substance in vitro. This means that it can produce DNA disturbances in vivo. Therefore, malathion can be regarded as a potential mutagen/carcinogen and requires further investigation.

12

Application of the comet assay in studies of programmed cell death [PCD] in plants

84%

Lesniewska J., Simeonova E., Sikora A., Mostowska A., Charzynska M.

Acta Societatis Botanicorum Poloniae

|

2000

|

tom 69

|

nr 2

13

Responsiveness of Lycopersicon pimpinellifolium to acute UV-C exposure: histo-cytochemistry of the injury and DNA damage

84%

Iriti M., Guarnieri S., Faoro F.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 2

The in vivo and in vitro effects of UV-C (254 nm) exposure (0.039 watt · m-2 · s for 2 h) of currant tomato (Lycopersicon pimpinellifolium), indigenous to Peru and Ecuador, were assayed. H2O2 deposits, dead cells and DNA damage were localized, 12/24 h after irradiation, mainly in periveinal parenchyma of the 1st and 2nd order veins of the leaves, and before the appearance of visible symptoms, which occurred 48 h after irradiation. Cell death index was of 43.5 ± 12% in exposed leaf tissues, 24 h after treatment. In currant tomato protoplasts, the percentage of viable cells dropped 1 h after UV-C irradiation from 97.42 ± 2.1% to 43.38 ± 4.2%. Afterwards, the protoplast viability progressively decreased to 40.16 ± 7.25% at 2 h, to 38.31 ± 6.9% at 4 h, and to 36.46 ± 1.84% at 6 h after the exposure. The genotoxic impact of UV-C radiation on protoplasts was assessed with single cell gel electrophoresis (SCGE, or comet assay). UV-C treatment greatly enhanced DNA migration, with 75.37 ± 3.7% of DNA in the tail versus 7.88 ± 5.5% in the case of untreated nuclei. Oxidative stress by H2O2, used as a positive control, induced a similar damage on non-irradiated protoplasts, with 71.59 ± 5.5% of DNA in the tail, whereas oxidative stress imposed on UV-C irradiated protoplasts slightly increased the DNA damage (85.13 ± 4.1%). According to these results, SCGE of protoplasts could be an alternative to nuclei extraction directly from leaf tissues.

14

Level of DNA damage in lead-exposed workers

67%

Olewinska E., Kasperczyk A., Kapka L., Kozlowska A., Pawlas N., Dobrakowski M., Birkner E., Kasperczyk S.

Annals of Agricultural and Environmental Medicine

|

2010

|

tom 17

|

nr 2

231-236

15

Apoptosis in preimplantation bovine embryos and methods used for its detection

67%

Opiela J.

Annals of Animal Science

|

2009

|

tom 09

|

nr 1

3-16

16

Lymphocyte DNA damage in rats challenged with a single bout of strenuous exercise

67%

Wierzba T. H., Olek R. A., Fedeli D., Falcioni G.

Journal of Physiology and Pharmacology. Supplement

|

2006

|

tom 57

|

nr 10

115-131

Exercise induces extensive generation of reactive oxygen species, which are responsible for tissue damage: enzymes inactivation, lipid peroxidation and single strand breaks in DNA. Defense system against free radicals is consisting of enzymes such as superoxide dismutase, catalase, glutathione peroxidase, and numerous non-enzymatic antioxidants. The study was performed to evaluate the effect of a single bout of submaximal running exercise, on the lymphocyte DNA strand breaks and also to test how supplementation with tempol – a membrane-permeable SOD-mimetic (0.2 mmol/kg/day) influences the eventually evoked damage. Male, Wistar rats were challenged with graded 50 min. running on treadmill at intensity up to 75-85% of predicted O2 max. The DNA strand breaks in individual lymphocytes were determined by using a gel electrophoretic technique – “comet” assay. We found substantial lymphocyte DNA damage 60 min. after the exercise. Tempol failed to prevent from oxidative damage in rats challenged with exercise. Moreover tempol by itself induced higher DNA damage than the exercise bout.

17

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results

67%

Milic M., Rozgaj R., Kasuba V., Orescanin V., Balija M., Jukic I.

Acta Biochimica Polonica

|

2010

|

tom 57

|

nr 3

The aim of this study was to find correlations between folate and vitamin B12 on baseline damage in white blood cells and their association with smoking, alcohol consumption and ageing. Thirty-six healthy vitamin non-deficient male subjects were selected in a randomized study. Comet assay (SCGE) and micronucleus (MN) assay were used as biomarkers of DNA damage. The amount of DNA damage was correlated with vitamin B12 and folic acid concentration. Positive, but non-significant correlation (canonical R = 0.61; χ2 = 28.97; P = 0.253) was found between micronucleus (MN) frequency or comet assay parameters (SCGE) and five covariates (age, smoking, alcohol consumption, vitamin B12 and folate blood serum concentration). The highest MN frequency was observed in the group with the lowest vitamin B12 concentration (F = 3.59; P = 0.024). The SCGE assay failed to show significant correlation with vitamin B12 or folic acid concentration. Concentration of vitamin B12 was significantly correlated with incidence of micronuclei. Our results present background data that could be valuable for future genotoxicological monitoring.

18

Evaluation of HI-6 oxime: potential use in protection of human acetylcholinesterase inhibited by antineoplastic drug irinotecan and its cyto-genotoxicity in vitro

67%

Radic B., Vrdoljak A. L., Zeljezic D., Fuchs N., Berend S., Kopjar N.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 3

The function of acetylcholinesterase (AChE) is the rapid hydrolysis of the neurotransmitter acetylcholine (ACh), which is involved in the numerous cholinergic pathways in both the central and the peripheral nervous system. Therefore, AChE measurement is of high value for therapy management, especially during the course of intoxication with different chemicals or drugs that inhibit the enzyme. Pyridinium or bispyridinium aldoximes (oximes) are able to recover the activity of the inhibited enzyme. Since their adverse effects are not well elucidated, in this study the efficiency of HI-6 oxime in protection and/or reactivation of human erythrocyte AChE inhibited by the antineoplastic drug irinotecan as well as its cyto/genotoxicity in vitro were investigated. HI-6 was effective in protection of AChE and increased its activity up to 30%; the residual activity after irinotecan inhibition was 7%. Also, it reactivated the enzyme previously inhibited by 50% irinotecan (4.6 µg/ml) applied at ¼ of the IC50 value. The tested concentrations of HI-6 exhibited acceptable genotoxicity towards white blood cells, as estimated by the alkaline comet assay, DNA diffusion assay and cytogenetic endpoints (structural chromosome aberrations and cytokinesis-block micronucleus assay). The results obtained warrant the further investigation of HI-6 in vivo, as well as its development for possible application in chemotherapy.

19

The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women

67%

Kapiszewska M., Kalemba M., Grzesiak A., Kocemba K.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 2

The aim of this study was to evaluate whether the differences in plasma 17β-estradiol concentration in early and late follicular phases of the menstrual cycle can affect the level of endogenous DNA damage in lymphocytes assessed by comet assay, and whether the extent of this damage in the follicular phase is associated with the genotype of catechol-O-methyltransferase (COMT). The level of DNA damage was positively correlated with 17β-estradiol concentration only in the late follicular phase. Subjects with the COMT L/L homozygous mutated variant revealed more DNA damage as compared to individuals with the COMT wild-type and heterozygous (H/L+HH) genotype.

20

Beta-carotene enhances the recovery of lymphocytes from oxidative DNA damage

67%

Fillion L., Collins A., Southon S.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 1

Epidemiological studies have revealed a strong correlation between high intake of fruit and vegetables and low incidence of certain cancers. Micronutrients present in these foods are thought to decrease free radical attack on DNA and hence protect against mutations that cause cancer, but the fine details of the causal mechanism have still to be elucidated. Whether dietary factors can modulate DNA repair - a crucial element in the avoidance of carcinogenesis - is an intriguing question that has not yet been satisfactorily answered. In order to investigate the effects of β-carotene on oxidative damage and its repair, volunteers were given a single 45 mg dose and lymphocytes taken before and after the supplement were treated in vitro with H2O2. DNA strand breaks and oxidised pyrimidines were measured at intervals, to monitor the removal of oxidative DNA damage. We found inter-individual variations in response. In cases where the baseline plasma β-carotene concentration was high, or where supplementation increased the plasma concentration, recovery from oxidative damage (i.e. removal of both oxidised pyrimidines and strand breaks) was relatively rapid. However, what seems to be an enhancement of repair might in fact represent an amelioration of the continuing oxidative stress encountered by the lymphocytes under in vitro culture conditions. We found that culture in a 5% oxygen atmosphere enhanced recovery of lymphocytes from H2O2 damage.

Ograniczanie wyników

The protective ability of the Mediterranean plant extracts against the oxidative DNA damage. The role of the radical oxygen species and the polyphenol content

Elektroforeza pojedynczych komorek [comet assay] - uzyteczna technika badania uszkodzen DNA

Is concentration and motility of male gametes related to DNA damage measured by comet assay?

Structural damage to nuclear DNA in mammalian spermatozoa: its evaluation techniques and relationship with male infertility

Differential resistance of human embryonic stem cells and somatic cell types to hydrogen peroxide-induced genotoxicity may be dependent on innate basal intracellular ROS levels

Methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies

The estrogenic status and the COMT genotype of female blood donors influence the protective ability of the Mediterranean plant extracts against the hydrogen peroxide-induced DNA damage in lymphocytes

Spontaneous DNA damage in lymphocytes of fox species

Neuroprotective effects of onion and garlic root extracts against Alzheimer’s disease in rats: antimicrobial, histopathological, and molecular studies

DNA damage in mouse lymphocytes exposed to curcumin and copper

Single cell gel electrophoresis [comet assay] as a tool for environmental biomonitoring. An example of pesticides

Application of the comet assay in studies of programmed cell death [PCD] in plants

Responsiveness of Lycopersicon pimpinellifolium to acute UV-C exposure: histo-cytochemistry of the injury and DNA damage

Level of DNA damage in lead-exposed workers

Apoptosis in preimplantation bovine embryos and methods used for its detection

Lymphocyte DNA damage in rats challenged with a single bout of strenuous exercise

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results

Evaluation of HI-6 oxime: potential use in protection of human acetylcholinesterase inhibited by antineoplastic drug irinotecan and its cyto-genotoxicity in vitro

The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women

Beta-carotene enhances the recovery of lymphocytes from oxidative DNA damage