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Predators are supposed to exert strong selection pressures on their prey, especially when phenotypic traits such as secondary sexual characters promote mating success at the expense of costs in terms of natural selection. Signaling theory predicts that individuals of superior phenotypic quality will enjoy an advantage in term of mating success, but also in term of natural selection, if such individuals are in prime condition both before and after development of exaggerated secondary sexual characters. We tested this prediction in the Barn Swallow Hirundo rustica being preyed upon by the Eurasian Sparrowhawk Accipiter nisus, using extensive samples of feathers from prey and non-prey. We measured tail length and coloration of outermost tail feathers in the black area of the proximal and distal part of tail feathers, but also the white spot of the tail feathers. Prey had significantly less dark distal, but not proximal parts of their tails, while there was no difference in coloration of the white spot between prey and non-prey. Prey had significantly paler tail feathers than non-prey, especially among long-tailed individuals. These results suggest that Barn Swallows with long tails that fail to deposit large amounts of melanin in their tail feathers run an elevated risk of predation.
Characterisation of salmon (Salmo salar) aged 0+, 1+, 2+, stocked in Pomeranian rivers, involved 25 plastic and 13 meristic characters, the opercular bone arrangement, and coloration. Coloration of body and fins, appearing at the age of 0+ at the fork length of 6-10 cm belonged to the earliest species-specific traits. Typical values of certain species-specific characters, such as tail base width, caudal fin incision (as per cent of l. caudalis), and the length of the upper jaw (as per cent of l. capitis) were recorded as early as in the fish aged 0+. Other characters, such as the opercular bone arrangement, were observed in those individuals aged 1+ and only in some fish aged 0+. The typical salmon anal fin shape occurred in some of the salmon aged 1+ and 2+ only. The study showed a high variability and significant differences in meristic and plastic characters between individuals grown in various rivers.
Colour is traditionally one of the important appearance features of all fruit for consumers in deciding to buy them. Colour is therefore important in the postharvest supply chain. But where does that colour of fruit come from? Clearly the period of growing and the circumstances during growth are important for developing this im­portant feature. During several seasons (2007-2009), the skin colour of individual apples of dif­ferent cultivars ('Braeburn', 'Fuji', 'Gala', 'Golden Delicious') were measured using a Minolta CR-400 chromameter during the last 40-60 days before (commercial) har­vest. By including the biological variation between individual apples in the analyses and applying non linear indexed regression analysis based on process oriented mod­els, explained parts were obtained for the a*-value, all exceeding 90%. The estimated rate constants for the colouration process were remarkably similar for all cultivars (except 'Fuji') and growing conditions. That would indicate that the process of colouration is really reflecting the degradation of chlorophyll and not the production of red or yellow coloured blush (anthocyanins). The expected effect of growing conditions (fertilization and crop level, hail net or not, sunny side or shady side of the tree) did change the mechanism nor the kinetic parameter values but could all be attributed to the minimal obtainable skin colour (asymptotic values of the logis­tic model). This type of information from the production period may constitute an important link to postharvest supply chain management.
Polyphenol oxidases (PPOs) reveal a range of forms and occur in all plants and crops. PPOs are comprised of three enzymes (catecholase, laccase, cresolase) with very different activities and specificities. Cresolase has a dualistic form (cresolase is only in plants and tyrosinase is only in animals and microorganisms). Very often in the literature the generic word "PPO" is used inappropriately as one enzyme. This should be avoided in future studies, as clear systematics and correct nomenclature of PPOs are needed for proper research. PPOs have different substrate specificities and typical inhibitors, and they catalyze hydroxylation and oxidation processes in plants. Pigment formation in cells and cellular systems is affected by active PPOs. Catecholases, laccases and cresolases are encoded by nuclear genes of plants. Various PPO DNA sequences have been found, and PPOs occur in multiple gene families. The protective potential of PPOs in plants and enhanced herbivory resistance is debated, and the final evidence has not yet appeared. The activity of PPOs in germination is recognized, but its mechanism is still not clear. Seed testa coloration in Arabidopsis thaliana is effected by laccase and not by catecholase. The TT10 gene encoding laccase in the Arabidopsis seed testa has been isolated. Arabidopsis genome analysis led to the identification of 16 other putative laccases and their genes, named AtLAC1 to AtLAC17 according to their position in the genome. Challenging areas of research for the future are seed testa PPOs and their mobilization in endosperm and micropylar regions, and PPOs as a part of the plant defense system and immunity.
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