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Usefulness of neutral red uptake method for investigation of the bovine leukocyte viability and lymphocyte proliferation

100%

Fijalkowski K., Nawrotek P., Czernomysy-Furowicz D.

Advances in Agricultural Sciences

2011

tom 14

nr 1-2

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Cytotoxic activity of Paris quadrifolia extract and isolated saponin fractions against human tumor cell lines

100%

Stefanowicz-Hajduk J., Kawiak A., Gajdus J., Ochocka J. R., Paszkiewicz M., Stepnowski P., Lojkowska E.

Acta Biologica Cracoviensia. Series Botanica

2011

tom 53

nr 2

Steroidal saponins isolated from many plant species belonging to Monocotyledones display potent cytotoxic activity towards many human tumor cells. We examined the cytotoxic effects of crude Paris quadrifolia extract for the first time, testing isolated saponin-rich fractions against four different human cell lines using the [(3-(4,5- dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxic activity was tested against human promyelocytic leukemia (HL-60) cells, human cervical adenocarcinoma (HeLa) cells and human breast cancer (MDA-MB-468) cells. Human skin fibroblasts were used as non-neoplastic control cells. Our results show significant activity of the weakly water-soluble solid residue and butanolic fraction against HL-60 and HeLa cells. The solid residue exerted cytotoxicity against all tested cell lines.

Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm

80%

Masojc P., Lapinski M., Larsson-Raznikiewicz M.

Journal of Applied Genetics

1995

tom 36

nr 3

α-Amylase isozymes from rye endosperm were analysed by means of isoelectric focusing, polyacrylamide gel electrophoresis, immunoelectrophoresis and colorimetric assay. Α-AMY 1 (high pI) group was separated into 13 IEF bands, whereas in group α-AMY2 (low pI) 2 intensive and 6-8 faint bands were found. Two linked (2±1 cM) polymorphic loci and a single locus with two alleles encoding for α-AMY 1 and α-AMY 2 groups, respectively, were identified after genetic analysis of the IEF patterns. All α-amylase isozymes developed on PAGE, were shown to belong to α-AMY 1 group. It was demonstrated that a single PAGE isozyme corresponds to 2-4 separate IEF bands and that most of the IEF bands can be attributed to more than one PAGE isozyme. The activity of α-amylases from PAGE zone I was 2.3 times higher than the activity of zone II isozymes. A strong correlation between the activity and protein amount of particular α-AMY 1 isozymes (r=0.94) was found.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Advances in Agricultural Sciences

1 Journal of Applied Genetics

1 Czernomysy-Furowicz D.

1 Fijalkowski K.

1 Gajdus J.

1 Kawiak A.

1 Lapinski M.

1 Larsson-Raznikiewicz M.

1 Lojkowska E.

1 Masojc P.

1 Nawrotek P.

1 Ochocka J. R.

1 Paszkiewicz M.

1 Stefanowicz-Hajduk J.

1 Stepnowski P.

2 2011

1 1995

Wyniki wyszukiwania

Usefulness of neutral red uptake method for investigation of the bovine leukocyte viability and lymphocyte proliferation

Cytotoxic activity of Paris quadrifolia extract and isolated saponin fractions against human tumor cell lines

Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm