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The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

100%

Basson M. D., Zeng B., Wang S.

Journal of Physiology and Pharmacology

2017

tom 68

nr 3

Comparison of phenolic compounds, antioxidant and cytotoxic activity of extracts prepared from Japanese quince (Chaenomeles japonica L.) leaves

84%

Chojnacka K., Sosnowska D., Polka D., Owczarek K., Gorlach-Lira K., De Verasa B. O., Lewandowska U.

Journal of Physiology and Pharmacology

2020

tom 71

nr 2

Molecular mechanism of protection against chemically and gamma-radiation induced apoptosis in human colon cancer cells

67%

Pierzchalski P., Krawiec A., Gawelko J., Pawlik W. W., Konturek S. J., Gonciarz M.

Journal of Physiology and Pharmacology. Supplement

2008

tom 59

nr 2

The involvement of peroxisome proliferator-activated receptors (PPARs) in the cancer cell elimination through apoptosis is a generally accepted fact. However, some reports indicate that the activation of PPAR is directly responsible for carcinogenesis. Caco-2 cells, a human adenocarcinoma cells, were used as a model of colon cancer. Cell cultures (5x106 cell per dish) were pretreated for 24h with PPARagonists ciglitazone (CI, 1x10-6M) and retinoic acid (RA, 1x10-6M) and part of the cultures were subsequently subjected to -radiation (photons) with therapeutic dose of 2,5 Gy. Total cellular RNA and proteins (cytoplasmic and nuclear) were isolated 24h after cultures irradiation or 48h after stimulation in the non irradiated part of experiment to preserve the equal growth time for all samples. -Irradiation of the cells abolished nuclear translocation of PPARunder its agonists treatment and preserved PPARin the cytoplasmic pool. But it did not affect the HSP 70 expression in response to ciglitazone and retinoic acid. Moreover, combined -irradiation and CI/RA treatment of the cells changed the equilibrium between Bax and Bcl-2 mRNA to anti apoptotic state with increased expression of Bcl-2 and almost abolished expression of Bax. In conclusion, this paper provides an evidence for the anti-apoptotic action of PPARagonists used along with the -radiation. Moreover, it shows that the up-regulated HSP70, in response to PPARagonists in -irradiated cultures promotes cell survival.

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Journal of Physiology and Pharmacology. Supplement

1 Basson M. D.

1 Chojnacka K.

1 De Verasa B. O.

1 Gawelko J.

1 Gonciarz M.

1 Gorlach-Lira K.

1 Konturek S. J.

1 Krawiec A.

1 Lewandowska U.

1 Owczarek K.

1 Pawlik W. W.

1 Pierzchalski P.

1 Polka D.

1 Sosnowska D.

1 Wang S.

1 Zeng B.

1 2020

1 2017

1 2008

Wyniki wyszukiwania

The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

Comparison of phenolic compounds, antioxidant and cytotoxic activity of extracts prepared from Japanese quince (Chaenomeles japonica L.) leaves

Molecular mechanism of protection against chemically and gamma-radiation induced apoptosis in human colon cancer cells