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1

Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets

100%
Koc I., Akdemir H., Onay A., Ciftci Y. O.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 09
Genetic stability of plants during in vitro propagation and conservation is one of the important aspects of plant biotechnology. In the present study, micropropagated P. lentiscus L. shoot cultures, which are cultivated for the mastic resin, have been cold stored up to 12 months at 4°C in the dark for different durations (2, 4, 6, 8, 10 and 12 months) and genetic alterations in cold storage conditions were evaluated. Growth parameters such as proliferation rate, shoot numbers per explant, shoot lengths and shoot forming capacity were also calculated. Since the highest proliferation rate (100 %) was obtained in 6 month-stored shoot cultures without any severe influence of cold stress on proliferation ability, amplified fragment length polymorphism (AFLP) and inter-retrotransposon amplified polymorphism (IRAP) marker systems were used to determine genetic stability in the plantlets after this storage period. Totally, 702 scorable bands were produced by 10 AFLP primer pairs. Genetic similarity value of the non-stored (control) plant and coldstored clones ranged from 0.66 to 0.84 with a mean of 0.74. In the case of IRAP, 159 bands were produced by 8 IRAP primers. Genetic similarity value of the non-stored plant and cold-stored clones varied from 0.65 to 0.83 and the average genetic similarity value was determined as 0.72. The genetic similarity indices revealed that genetic variability was similar in both techniques. Our results showed that tissue culture and especially cold storage of P. lentiscus L. may result transposons activation, thus could cause genetic instability.
2

Transcriptomic analysis of cold response in tomato fruits identifies dehydrin as a marker of cold stress

86%
Weiss J., Egea-Cortines M.
Journal of Applied Genetics
|
2009
|
tom 50
|
nr 4
311-319
Tomato is sensitive to cold during vegetative growth, fruit set, development, and ripening. We have characterized the effect of cold stress (6°C for up to 48 h) on the transcriptome of Micro-Tom tomato fruits during ripening by subtractive PCR. The cold stress caused modifications in gene expression of housekeeping genes. From a total of 38 genes up-regulated by cold, only one clone - a dehydrin homologue - was related to previously identified cold-stress genes. Phylogenetic analysis showed its clustering with other cold-induced dehydrins, and increased distances from dehydrins activated by abscisic acid. Quantitative expression analysis of tomato dehydrin showed it was activated by cold treatment in leaves and fruits. As dehydrin is a member of the Sl-CBF1 regulon from tomato, we analyzed the cold-responsive transcription factor Sl-CBF1 in mature leaves and ripening fruits stored at 6°C. Leaves of Micro-Tom showed high basal levels of the transcription factor Sl-CBF1, compared to fruits. Cold treatment caused increased levels of Sl-CBF1 expression in leaves but not in fruits of Micro-Tom and Demisem (a commercial cultivar). Tomato dehydrin can be used as a transcriptional marker of cold stress in leaves and ripening fruits. However, our results indicate that the cold response activation of dehydrin gene in tomato fruits is the consequence of an alternative pathway, different from the Sl-CBF1 regulon.
3

Effect of low temperature stress on plants: physiological and molecular aspects

86%
Kravets V.
Biological Bulletin of Poznań. Supplement
|
1997
|
tom 34
4

The accumulation of raffinose in vegetative tissues of legume seedlings in response to cold and desiccation stress

72%
Lahuta L. B., Goszczynska J., Gorecki R. J.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
5
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Changes in the expression of three cold-regulated genes in "Elsanta" and "Selvik" strawberry (Fragaria × ananassa) plants exposed to freezing

72%
Badek B., Napiorkowska B., Masny A., Korbin M.
Journal of Horticultural Research
|
2014
|
tom 22
|
nr 2
6
Content available remote

Influence of forced treadmill and voluntary wheel running on the sensitivity of gastric mucosa to ulcerogenic stimuli in male rats

72%
Yarushkina N. I., Komkova O. P., Filaretova L. P.
Journal of Physiology and Pharmacology
|
2020
|
tom 71
|
nr 6
7

Molecular cloning, characterization and expression analysis of PtrHOS1, a novel gene of cold responses from trifoliate orange [Poncirus trifoliata (L.) Raf.]

72%
Liu D.-C., He L.-G., Wang H.-L., Xu M., Sun Z.-H.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 2
High expression of osmotically responsive genes 1 (HOS1) encodes an ubiquitin E3 ligase that promotes the degradation of transcription factor Inducer of CBF Expression 1 (ICE1). Inactivation of ICE1 reduces CBF-induced activation of many cold-responsive genes, and thus, HOS1 act as a negative regulator of cold-responsive genes. In this paper, a novel HOS1 gene, designated PtrHOS1 (Genebank accession number FJ844367), was cloned by RT-PCR and RACE-PCR from trifoliate orange [Poncirus trifoliata (L.) Raf.]. The full length of PtrHOS1 is 3,434 bp with an open reading frame of 2,922 bp, encoding a protein of 974 amino acids with a molecular weight of 110.2 kDa and a theoretical isoelectric point of 5.55. Sequence alignment showed that PtrHOS1 protein had a conserved RING finger domain in its N-terminal region and shared high identity with other plant species HOS1-like proteins. Semi-quantitative RT-PCR analysis revealed that PtrHOS1 could be constitutively expressed at high levels in leaves, stems and roots. Interestingly, the PtrHOS1 expression had a declined period in leaves, stems and roots after cold and ABA treatments, which suggested that the PtrHOS1 expression was down regulated both by cold and ABA. Moreover, the decline was first occurred in leaves (30 min), followed with stems (2 h) and roots (4 h) after cold treatments. These results probably suggest that the leaves of trifoliate orange first sense the cold stress, followed with stems and roots. Oppositely, after ABA treatments, the significant decline of PtrHOS1 expression was first occurred in roots (15 min), followed with stems and leaves (30 min). Our results provide useful information for further studies about cold acclimation mechanism in citrus.
8
Content available remote

Effects of two types of restraint stress on spontaneous behavior of Sprague-Dawley and Lewis rats

72%
Klenerova V., Sida P., Krejci I., Hlinak Z., Hynie S.
Journal of Physiology and Pharmacology
|
2007
|
tom 58
|
nr 1
The purpose of this study was to evaluate the action of two types of stressors in Sprague-Dawley (S-D) and Lewis (LEW) rats differing in their hypothalamic-pituitary-adrenal axis activity on locomotion and rearing in an open space. Exposure to restraint immobilization alone (IMO) or this immobilization combined with cold water (22 °C) immersion (IMO+C) lasted for 1 h and started 2 or 5 h before the test. To evaluate the acute and persisting effects of both stressors, four trials were performed in one-week intervals; rats were exposed to the stressors in trial 1 and 3. While in LEW rats both acute stressors produced reduction of locomotion and rearing in all stressed groups, S-D rats responded with a decrease of both parameters only after IMO+C presented 2 h before testing. Neither strain displayed a changed performance one week after the first stress exposure. One week after the second stress exposure rats of both strains exhibited a tendency to an increase of both parameters reaching the significance in several experimental groups. The findings indicate: a) the IMO+C produced stronger behavioral alteration than IMO alone; b) the behavioral responses to stressors were more pronounced in LEW compared to S-D strain; c) change from the reduction of activity to its increase may be interpreted as bi-directional manifestation of long-term effects of immobilization stress.
9

Influence of cold stress on ultrastructure and protein synthesis in seeds of Acer tataricum L.

72%
Babenko L., Martyn G., Musatenko L.
Biological Bulletin of Poznań. Supplement
|
1997
|
tom 34
10

Effect of supplemental vitamin C and cold stress on performance of broiler chickens

72%
Sosnowka-Czajka E., Skomorucha I., Herbut E.
Annals of Animal Science. Supplement
|
2003
|
nr 2
11

Evidence of climatic adaptation in spontaneous torpor among Pouched mice Saccostomus campestris from southern Africa

72%
Ellison G. T. H.
Acta Theriologica
|
1993
|
tom 38
|
nr 1
Spontaneous torpor was examined in 36 Pouched mice Soccosiomi/s campestris Peters, 1846 from 6 localities in southern Africa. Fifteen .individuals displayed characteristic bouts of torpor in which oxygen consumption (VO2) fell below 50% of the resting levels of non-torpid pouched mice for more than 2 hr during the early morning. These bouts ended in an abrupt increase in VO2 which oversfhot resting levels during arousal to normothermia. Minimum oxygen consumption (V02min) was not correlated with the energy saved during torpor, but there was a significant positive correlation between V02min and the duration of torpor (DO and also between Dt and energy saved. In contrast, there was a negative correlation between the cost of arousal and energy saved which suggests that the cost of regaining homeothermy can limit the energetic efficiency of short bouts of torpor. Pouched mice from all 6 localities had the capacity to enter torpor and there were no significant geographical differences in the incidence of torpor. However, there were substantial differences in the expression of torpor, and Pouched mice from localities experiencing more variable temperatures underwent significantly longer bouts of torpor which suggests that selection for torpor had been greater in more energetically unpredictable environments.
12

Effects of growth regulators and cold stress on growth and proline content of bean seedlings

72%
Demir Y.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2002
|
tom 50
|
nr 1
13

Response of antioxidative enzymes to long term cold and light stress of spruce seedlings

58%
Kaminska-Rozek E., Pukacka P. M.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effects of different intensities of cold stress on certain physiological phenomena related to skeletal health in a hypogonadal rat model

58%
Islam M. N., Chanda S., Mitra C.
Journal of Physiology and Pharmacology
|
2000
|
tom 51
|
nr 4,2
15

Correlation between chloroplast motility and elastic properties of tobacco mesophyll protoplasts

58%
Augustynowicz J., Lekka M., Burda K., Gabrys H.
Acta Physiologiae Plantarum
|
2001
|
tom 23
|
nr 3
16

The effect of cold temperature on tonic immobility [TI] and heterophil to lymphocyte ratio [H:L] in male broilers

58%
Ozkan S., Yalcyn S., Guler C., Kozanoglu H., Cabuk M., Basmacyoglu H.
Animal Science Papers and Reports. Supplement
|
2005
|
tom 23
|
nr 1
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Molecular study on cold- and drought resistance in barley

58%
Cattivelli L., Crosatti C., Grossi M., Faccioli P., Stanca A. M.
Genetica Polonica
|
1994
|
tom 35B
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