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1

The cold shock response in microorganisms

100%

Wolska K. I.

Acta Biochimica Polonica

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1994

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tom 41

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nr 4

2

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Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees Celsius

63%

Dziekonska A., Strzezek J.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Metabolic activity of boar spermatozoa, liquid stored for three days at 5℃, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5℃ may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

3

Artificial gynogenesis in common bream (Abramis brama (L.)) induced by cold-temperature shock

63%

Kucharczyk D., Luczynski M. J., Szczerbowski A., Woznicki P., Luczynski M., Targonska-Dietrich K., Kujawa R. J., Mamcarz A.

Archives of Polish Fisheries

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2004

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tom 12

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nr 2

Bream, Abramis brama (L.), eggs fertilized with genetically inactivated sperm (UV irradiation dose of 1920 J m-2) were exposed to thermal cold shock to produce meiotic gynogenotes. The shock was applied at one-minute intervals from 1 to 10 min after egg insemination. The temperature of the shock was 2.0 ± 0.1°C, and its duration was 45 min. The water temperature prior to the shock was 20.0°C. Eggs fertilized with genetically inactivated sperm (putative haploids) exhibited retarded and abnormal development. The yield of gynogenesis was relatively low, except for the group to which the shock was applied 1 min after fertilization (about 30% in comparison with the controls). Ninety fish from the control and gynogenetic groups were reared for ten months. The survival of the gynogenetic bream was twofold lower than that of the controls. The gynogenotes were highly variable in size and exhibited some morphological abnormalities. The sex ratios in the control groups were close to 1:1, whereas all the gynogenotes were female.

4

Low temperature induction of genes of cold shock proteins in Brassica oleracea and some other representatives of Cruciferae family

63%

Gimalov F. R., Baimiev A. K., Chemeris A. V., Vakhitov V. A.

Biological Bulletin of Poznań. Supplement

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1997

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tom 34

5

Comparison of effectiveness of thermal heat and cold shocks applied in the gynogenetic reproduction of common carp [Cyprinus carpio L.]

63%

Bialowas H.

Acta Hydrobiologica

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1995

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tom 37

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nr 2

The effects of heat (40 °C, 1.5 min) and cold (2-4 °C, 60 min) shocks applied within 2nd, 8th, 15, and 30th minute after fertilization on the results of gynogenetic reproduction of common carp were compared. The sperm was inactivated using ultraviolet radiation. The temperature of fertilization and incubation of eggs was 22 °C. Greater numbers of larvae were obtained in the case of the heat shocks (0.3-3.4% of the incubated eggs) than of the cold ones (0-0.4%).

6

Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen

63%

Nur Z., Segirkaya H., Dogan I., Soylu M. K., Ak K., Ileri I. K.

Medycyna Weterynaryjna

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2005

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tom 61

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nr 09

7

Effects of different temperature treatments applied to deep stored bull semen and post-thaw cold shocked spermatozoa

51%

Nur Z., Ileri I. K., Ak K.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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