Wyniki wyszukiwania

1

Polymorphism in coding and non-coding regions of the MyoD gene family and meat quality in pigs

100%

Kapelanski W., Grajewska S., Kuryl J., Bocian M., Wyszynska-Koko J., Urbanski P.

Folia Biologica

|

2005

|

tom 53

|

nr Suppl.

2

Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain

84%

Loniewska-Lwowska A., Chelstowska S., Zagorski-Ostoja W., Palucha A.

Acta Biochimica Polonica

|

2009

|

tom 56

|

nr 4

619-625

Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukaryotic cells. Here, we demonstrate, that in wheat germ extract (WGE) truncation of the sgRNA1 5' UTR increases translation efficiency, as it has previously been observed in rabbit reticulocyte lysate (RRL), whereas removal of the 3' UTR does not affect translation. We also describe two regulatory elements located within the coding sequence of the coat protein (CP) gene and its read-through domain (RTD) and are responsible for regulation of in vitro translation of the PLRV sgRNA1. The first element is composed of the purine sequence AAAGGAAA located between the AUG codons of the CP and 17K genes. Deletion of this domain or its substitution by pyrimidines reduced by half the translation of both genes, whereas deletion of the RTD resulted in a 3.6-fold reduction in translation efficiency. This is the first report of translation regulatory elements of plant viruses located within a coding region.

3

Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits

84%

Huang Y.-Z., Zhang E.-P., Chen H., Wang J., Li Z.-J., Huai Y.-T., Ma L., Lan X.-Y., Ren G., Lei C.-Z., Fang X.-T., Wang J.-Q.

Journal of Applied Genetics

|

2010

|

tom 51

|

nr 2

199-202

The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and Polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P <0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.

4

Structure of the coding region and mRNA variants of the apyrase gene from pea [Pisum sativum]

84%

Shibata K., Abe S., Davies E.

Acta Physiologiae Plantarum

|

2001

|

tom 23

|

nr 1

5

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Prediction of indels and SNP’s in coding regions of glutathione peroxidases - an important enzyme in redox homeostasis of plants

67%

Ganguli S., Datta A.

International Letters of Natural Sciences

|

2014

|

tom 02

Plant glutathione peroxidases are an important class of enzymes which play key roles in the stress adaptability of plants both in context of biotic and abiotic stress pathways. They have been over the years much studied in animals since the catalytic residues are comprised of selenocysteine a variant amino acid which is ribosomally encoded with the help of an RNA structural element known as SECIS. Various workers over the years have shown that plant glutathione peroxidases play active roles in ROS sequestration, lipid hydroperoxidation as well as regulate glutathione levels. However, each plant has various patterns of glutathione peroxidase expression and action and in some plants certain isoforms have not been detected at all. This work focuses on the prediction and identification of single nucleotide polymorphisms (SNPs) and INDELs in the coding regions of plant glutathione peroxidases, with the help of a Bayesian based algorithm subsequently validated. A large number of informative sites were detected 279 of which had variant frequency of ≥ 50 %. This data should be beneficial for future studies involving genetic manipulation and population based breeding experiments.

6

Genetic analysis of Polish isolate of swine vesicular disease virus [SVDV POL-73] by RT-PCR and nucleotide sequencing of the VP1-2A coding region

67%

Niedbalski W.

Bulletin of the Veterinary Institute in Puławy

|

1998

|

tom 42

|

nr 1

A nucleotide sequence analysis by RT-PCR and cycle sequencing of the Polish isolate of swine vesicular disease virus (SVDV) was performed. The RT-PCR products pattern and VP1/2A coding region sequence were compared to the other available European isolates of SVDV and the human Coxackie virus isolate CAV-16. The results of the RT-PCR show that it is possible to detect different isolates of the SVDV however Coxackie virus-specific PCR products were obtained only by the use of one from three selected primer pairs. A sensitivity of the used RT-PCR was equivalent to that of virus isolation in cell culture and even higher than the ELISA method. The considerable nucleotide similarity between the Polish isolate UKG27/72 and the Japan 76 SVDV isolates (99% and 98%, respectively) as well as the CAV-16 (96%) was found. SVDV Poland 73 was genetically less related (about 88%) to the Dutch and Italian isolates from the recent outbreaks of disease in 1992. Presented results confirmed the quasispecies character of these picornaviruses.

Ograniczanie wyników

Polymorphism in coding and non-coding regions of the MyoD gene family and meat quality in pigs

Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain

Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits

Structure of the coding region and mRNA variants of the apyrase gene from pea [Pisum sativum]

Prediction of indels and SNP’s in coding regions of glutathione peroxidases - an important enzyme in redox homeostasis of plants

Genetic analysis of Polish isolate of swine vesicular disease virus [SVDV POL-73] by RT-PCR and nucleotide sequencing of the VP1-2A coding region