Wyniki wyszukiwania

1

Species-specific sensitivity of coagulase-negative staphylococci to single antibiotics and their combinations

100%

Szymanska G., Szemraj M., Szewczyk E. M.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 2

The activity of β-lactam antibiotics (oxacillin, cloxacillin, cephalotin), vancomycin, gentamicin and rifampicin applied in vitro individually and in combination against 37 nosocomial methicillin-resistant strains of coagulase-negative staphylococci (CNS) was assessed to demonstrate the heterogeneity of this group of bacteria and estimate the chance of the efficacy of such therapy. The strains belonged to four species: Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus cohnii, Staphylococcus hominis. They originated from a hospital environment and from the skin of medical staff of the intensive care unit of a paediatric ward at a university hospital. All strains were methicillin-resistant, according to CLSI standards, but individual strains differed in MICOX values. Susceptibility to other tested antibiotics was also characteristic for the species. The increased susceptibility to antibiotics in combinations, tested by calculating the fractional inhibitory concentration (FIC) index, concerned 26 out of 37 investigated strains and it was a feature of a particular species. Combinations of vancomycin and cephalotin against S. epidermidis and oxacillin with vancomycin were significant, as well as cephalotin and rifampicin in growth inhibition of multiresistant S. haemolyticus strains.

2

Molecular typing of Staphylococcus aureus isolated from cows with mastitis in the east of Poland on the basis of polymorphism of genes coding protein A and coagulase

80%

Jakubczak A., Szweda P., Lukaszewska K., Kur J.

Polish Journal of Veterinary Sciences

|

2007

|

tom 10

|

nr 4

The aim of this study was to test the diversity of a population of 82 strains of S. aureus isolated from cows with mastitis in the east of Poland. The isolates were typed by analysis of the number of repeats of 24 bp sequences in the X region of protein A (spa) gene and restriction fragment length polymorphism (RFLP) of the coagulase (coa) gene. Twelve different spa types were distinguished. Amplification of region X gave, in 79 cases, one stripe. In a scope of 100-364 bp 10 different products (genotypes) of amplification reaction were defined. For one strain two stripes were obtained and two strains did not contain the spa gene. The most prevalent strains had 10, 11 and 12 repeats of 24 bp sequences, which represented respectively 18%, 30% and 13% of all strains tested. The presence of any strain containing 4 or 9 sequence was not observed. In the case of analysis of the polymorphism of the coagulase gene, 13 different genotypes were identified. The most frequently appearing genotype is genotype C, in which case an amplification product is digested into three DNA fragments: 410, 320 and 160 bp. To this genotype belong 43 strains, which constitute 52% of the examined population. A significant improvement in discriminatory power was observed when results from both genes were analyzed simultaneously. In an analyzed group of 82 strains, 24 genotypes were isolated.

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Wpływ aureomycyny i terramycyny na wytwarzanie toksyn gronkowcowych

61%

Burzynska H.

Roczniki Państwowego Zakładu Higieny

|

1958

|

tom 09

|

nr 2

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Polish Journal of Veterinary Sciences

1 Roczniki Państwowego Zakładu Higieny

1 Burzynska H.

1 Jakubczak A.

1 Kur J.

1 Lukaszewska K.

1 Szemraj M.

1 Szewczyk E. M.

1 Szweda P.

1 Szymanska G.

1 2011

1 2007

1 1958

Species-specific sensitivity of coagulase-negative staphylococci to single antibiotics and their combinations

Molecular typing of Staphylococcus aureus isolated from cows with mastitis in the east of Poland on the basis of polymorphism of genes coding protein A and coagulase

Wpływ aureomycyny i terramycyny na wytwarzanie toksyn gronkowcowych